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Biomedical subjects

T Harder

Publications and source records attributed to T Harder.

At least 73 records · Page 4Linked to original sources
PubMed1992

Cloning and characterization of the human gene encoding p11: structural similarity to other members of the S-100 gene family.

The human gene (CLP11) encoding p11, the cellular ligand of the tyrosine kinase substrate, annexin II (AnxII), has been isolated from a human genomic library. Restriction mapping and sequencing reveals that CLP11 covers a stretch of approx. 11 kb in the human genome. The structure of CLP11 resembles that of the other genes encoding S-100 proteins which have been characterized so far: the transcribed region is divided by two introns, one in the 5'-nontranslated portion and the second in the protein-coding region. Interestingly, in CLP11, as well as all other S-100 genes, the second intron separates the codons for two corresponding amino acids, which reside in the sequence connecting the two helix-loop-helix (EF-hand) motifs. The 5'-nontranscribed region, which most likely represents the CLP11 promoter, is characterized by a high G+C content and probably is part of a CpG-island. Several putative binding sites for transcription factors can be identified in the 5'-nontranscribed region of CLP11. Among them, the beta DRE element, which was first described in the beta-globin promoter, is most notable, since it is also present in the promoter of ANXII. It could be responsible for the simultaneous induction of CLP11 and ANXII expression during certain cell differentiation processes, e.g., the nerve growth factor-induced differentiation of the pheochromocytoma cell line, PC12.

Amino Acid Sequence↗
PubMed1992

Round table on morbilliviruses in marine mammals.

Since 1988 morbilliviruses have been increasingly recognized and held responsible for mass mortality amongst harbour seals (Phoca vitulina) and other seal species. Virus isolations and characterization proved that morbilliviruses from seals in Northwest Europe were genetically distinct from other known members of this group including canine distemper virus (CDV), rinderpest virus, peste des petits ruminants virus and measles virus. An epidemic in Baikal seals in 1987 was apparently caused by a morbillivirus closely related to CDV so that two morbilliviruses have now been identified in two geographically distant seal populations, with only the group of isolates from Northwest Europe forming a new member of the genus morbillivirus: phocid distemper virus (PDV). Because of distemper-like disease, the Baikal seal morbillivirus was tentatively named PDV-2 in spite of its possible identity with CDV. The appearance of morbilliviruses in the Mediterranean Sea causing high mortality amongst dolphins should further increase the research activities on protection strategies for endangered species of marine mammals.

Animals↗
PubMed1991

[Inferior vena cava compression syndrome in cystic kidney. Duplex ultrasonic diagnosis].

Four months after renal transplantation for polycystic renal degeneration a 38-year-old man developed breathing-related pain in the left upper lung and a sinus tachycardia (130/min). Lung perfusion scintigraphy demonstrated pulmonary emboli from an acute venous thrombosis of the left lower leg. Polycythaemia and impairment of clot-inhibiting factors were excluded. Ultrasound examination of the abdomen revealed stenosis of the inferior vena cava (IVC) distal to the hepatic veins, dorsal to the liver and ventral of a huge right polycystic kidney which had been left in situ at the time of the renal transplantation. Duplex sonography demonstrated a band-like flow profile in the region of the stenosis. Blood flow was clearly increased (0.62 m/s) and not affected by either heart rate or breathing movements. The findings were confirmed by angiography. The right kidney, weighing 5 kg, was removed at surgery. The IVC stenosis was postoperatively found to be relieved and duplex sonography gave normal findings.

Adult↗
PubMed1991

[Non-selective digital subtraction angiography of aortocoronary bypasses].

Intra-arterial DSA was performed on 225 patients with a total of 552 coronary bypasses (515 aorto-coronary venous bypasses and 37 internal mammary artery bypasses). Four hundred and ninety-five bypasses were examined in the four weeks following surgery; of these, 428 (85.9%) were patent. Demonstration of the distal anastomosis was obtained in 40.4% of bypasses of the right anterior interventricular artery and in 36.1% of the right coronary artery, at least in their proximal parts. Bypasses of smaller branches showed filling in 12.8 to 19.2%. Because of the unsatisfactory demonstration of distal vessels by non-selective intra-arterial DSA, this method is suitable only for showing the patency of a bypass in the postoperative phase, but should not be used for investigating cardiac signs and symptoms following a bypass examination.

Adult↗
PubMed1991

Detection of phocid distemper virus RNA in seal tissues using slot hybridization and the polymerase chain reaction amplification assay: genetic evidence that the virus is distinct from canine distemper virus.

Slot hybridization and the polymerase chain reaction (PCR) after reverse transcription (RT) were used to detect RNA extracted from tissues of seals after naturally occurring disease and experimental infection with phocid distemper virus (PDV). A phosphoprotein (P) gene-specific cDNA served as a probe for both slot hybridization and the identification of PCR-generated fragments by Southern blotting. As primers for the PCR assay PDV P gene-derived oligonucleotides were used. Hybridization, PCR and partial nucleic acid sequence analysis clearly demonstrated that PDV is a distinct virus (most closely related to canine distemper virus) within the morbillivirus group. PCR, when combined with Southern blot hybridization, was clearly superior to slot hybridization and more sensitive than cell culture isolation and immunofluorescence assays for the detection of virus in tissues. Considerable amounts of viral RNA could be demonstrated in the lungs and spleens. In experimentally infected animals a large quantity of virus-specific RNA was additionally found in colon samples. Using RT-PCR in combination with Southern blotting. PDV could be demonstrated in buffy coat cells using a simple and fast cell lysis procedure.

Animals↗
PubMed1991

[Color-coded Doppler sonography of chronic venous insufficiency].

20 patients (i.e. 40 lower extremities) with chronic venous insufficiency were examined by means of colour-coded Doppler sonography (CCDS). This method enables the simultaneous visualisation of the anatomy and haemodynamics of the examined vessels. All cases of varicosis of the stem of the vena saphena magna (VSM) (n = 21) and of the vena saphena parva (VSP) (n = 13) were correctly diagnosed by CCDS. However, an incomplete varicosis of the stem was identified in only 3 of 5 cases. Insufficiency of the V. perforantes was identified by CCDS with a high rate of accuracy in diagnosing the insufficiency of the cross of the VSM and VSP. Although assessment of the V. perforantes and diagnosis of incomplete stem varicoses or varicoses of lateral branches is possible, as a rule, it does take up much time and requires a great deal of experience on the part of the investigator.

Adult↗
PubMed1991

[Diagnostic and therapeutic control of varicoceles using angiodynography].

We report on the results of angiodynography of 21 patients with suspected varicoceles. In 14 cases a varicocele could be diagnosed. Additionally, 19 patients underwent conventional Doppler sonography (16 with positive findings), 7 thereof also a testicular phlebography (5 positive findings). Thus, in conventional Doppler sonography there were 2 false positive findings. With angiodynography it was possible to detect an existing varicocele in all 14 (of the 21) cases even if only a small retrograde flow existed. In 11 of the cases this could be graded with the help of the flow velocity. In case of negative findings in angiodynography, further invasive techniques are unnecessary. However, before embolisation of a varicocele and to show preoperatively vessel variations or collateral vessels, testicular phlebography is still indicated.

Adult↗
PubMed1990

[In vivo measurement of tissue relaxation times using localized 31P and 1H MR spectroscopy].

Methods of in vivo determination of 31P longitudinal relaxation times within image-determined volume selected MRI spectroscopy have been investigated. T1 values for phosphor compounds in skeletal muscle and in brain have been measured. The 1H relaxation times of T1 and T2 of diffuse parenchymal liver disease, using inversion recovery and spin echo sequences were compared with quantitative MR tomography. Clinically, spectroscopic techniques are particularly useful where there is superimposition of water and lipid signals during MR imaging.

Brain↗
PubMed1990

[MR tomography and computer tomography of alcohol-induced brain tissue changes].

Results of MRI in five alcoholics suffering from acute neurologic disorders such as ataxia, ophthalmoplegia and confusion are presented. The detection of focal cerebral lesions of high signal intensity in T2-weighted Spin-Echo- and Gradient-Echo-images in periventricular white matter, thalamus and pons combined with patients history and clinical presentation led to the diagnosis of Wernicke-Encephalopathia and Pontine Myelinolysis. MRI was far more sensitive compared with CT.

Adult↗
PubMed1990

[Qualitative and quantitative NMR tomographic findings in focal nodular hyperplasia of the liver].

The qualitative and quantitative MRI findings in 16 patients with focal nodular hyperplasia (FNH) of the liver are described; nine of these were confirmed histologically. A central scar is typical of FNH and provides a reliable diagnosis. This finding was seen in half the cases. If the scar is not demonstrable, the following features suggest the diagnosis: smooth margins, homogeneous signal distribution, increased signal intensity in T2-weighted spin-echo images and reduced signal intensity in inversion-recovery sequences. The T1 and T2 relaxation times in FNH are increased by about 30% compared with normal liver tissue.

Adult↗
PubMed1990

[Qualitative control over quantitative MR tomography: in vitro and in vivo checks on relaxation time measurements].

In-vivo and in-vitro checks on measurements of relaxation times in MRI have been carried out as part of quality control. For the in-vitro experiments we used 13 reagents consisting of varying concentrations of copper sulphate manganese sulphate, gadolinium solutions and various oils as well as water. For in-vivo measurements we used liver parenchyma and fat in three subjects. Eight different sequences were performed; intra- and interindividual variations and comparison of three similar MRI units showed average variations of 2-3% for T1 and 3-4% variations for T2 relaxation times. This margin of error makes quantitative MRI acceptable for clinical use.

Adolescent↗
PubMed1990

Morbillivirus infections of seals during the 1988 epidemic in the Bay of Heligoland: III. Transmission studies of cell culture-propagated phocine distemper virus in harbour seals (Phoca vitulina) and a grey seal (Halichoerus grypus): clinical, virological and serological results.

Eight harbour seals (Phoca vitulina), two of them seronegative, six seropositive against PDV and a seronegative grey seal (Halichoerus grypus) were exposed to a low doses of a cell culture-propagated phocine distemper virus isolate (PDV 2558/Han 88). An intranasal route of inoculation was chosen. Clinical signs, resembling those of 1988's seal disease and seroconversion were observed in both seronegative harbour seals. One of them succumbed to the infection. The virus was not transmitted to another susceptible harbour seal which served as in-contact animal. Virus could be recovered from leucocytes of the diseased seals. Viremia was also present in a seropositive harbour seal that developed mild clinical signs; other seropositive seals were protected from clinical disease. The grey seal showed seroconversion upon inoculation, but did not develop any signs of disease. The humoral immune response of the seals plainly discriminated between homologous (PDV) and heterologous (canine distemper virus, CDV) virus as shown by virus neutralization tests and an antibody-binding assay (PLA).

Animals↗