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Biomedical subjects

T Harder

Publications and source records attributed to T Harder.

At least 91 records · Page 5Linked to original sources
PubMed1990

Detection of phocine distemper virus using the polymerase chain reaction.

During the fatal seal epizootics in the North and Baltic Seas in summer 1988 a virus was isolated which was shown to be the causal agent. It was subsequently classified as morbillivirus by neutralization assays, reaction with monoclonal antibodies and nucleic acid hybridization studies. The virus (tentatively called Phocine Distemper Virus, PDV) is difficult to grow in culture making rapid diagnosis difficult. We have used the Polymerase Chain Reaction (PCR) as an alternative and fast method to detect the presence of virus-specific nucleic acid and we describe here the amplification of cell culture derived PDV RNA in a "one-tube" reaction using heterologous (Rinderpest Virus cDNA derived) F gene primers. The resulting 370 bp DNA fragment was shown to be morbillivirus derived by Southern blot hybridization using cloned RPV F gene as probe.

Animals↗
PubMed1990

[Angiography findings before and following transplantation of free myocutaneous flaps].

Prerequisite for successful split-skin flap transplants of myocutaneous flaps is the presence of adequate adjacent afferent and efferent vessels, thus making an arteriography of the receiving region mandatory during surgery preparation. In 20 or 29 patients in whom a split myocutaneous flap was transplanted to the lower leg, angiography revealed 22 pathological or other therapyrelevant findings. Besides the afferent vessels it is basically necessary to visualise the peripheral efferent pathways in order to prevent that vascular anastomosis to the split-skin flap obstructs the blood supply of the leg.

Adolescent↗
PubMed1990

[Computed tomography in malignant primary bone tumors].

The importance of computed tomography is examined in malignant primary bone tumours using a strongly defined examination group of 13 Patients (six Ewing's-sarcomas, five osteosarcomas, one chondrosarcoma and one spindle-shaped cell sarcoma). Computed tomography is judged superior compared to plain radiographs in recognition of bone marrow infiltration and presentation of parosteal tumour parts as well as in analysis of tissue components of tumours, CT is especially suitable for therapy planning and evaluating response to therapy. CT does not provide sufficient diagnostic information to determine dignity and exact diagnosis of bone tumours.

Adolescent↗
PubMed1989

[MR tomography, CT and angiography of vascular malformations of the central nervous system].

The findings on MR tomography in 31 vascular malformations of the central nervous system are described; 26 of these had angiography and 24 were examined by CT. There were 19 A-V angiomas, 7 cavernomas and 5 large aneurysms. MRT provided a correct diagnosis in 29 out of 31 cases, angiography succeeded in 19 out of 26 cases and CT in 20 of 24. MRT was superior to CT and angiography. Nevertheless, it cannot replace arteriography, since this is the only method for demonstrating the vascular supply of angiomas and small aneurysms can be demonstrated only by arteriography.

Angiography↗
PubMed1989

[CT portography in malignant space-occupying lesions of the liver].

CT portography was performed in 32 patients with liver tumours and in four patients with cirrhosis of the liver, but without evidence of tumour, after angiographic injection of contrast into the superior mesenteric or splenic arteries. Compared with CT using no contrast, or contrast by intravenous injection, CT portography has the following advantages: better tumour detection, better detection of tumour size, better demarcation and localisation of tumours, better demonstration of the portal system, more inhomogeneity of the parenchyma. Of 12 patients with a number of tumours shown on CT, CT portography demonstrated tumours in four cases, which could not be shown by any other imaging method. Problems concerning CT portography in patients with cirrhosis of the liver are discussed.

Adult↗
PubMed1989

[MR tomography following craniocerebral trauma: comparison with computed tomography, electroencephalography and neurologic status].

56 patients with head and brain trauma and in coma were studied prospectively by means of MRT, CT, EEG and neurological examination. All patients had initial CT and EEG admission. MRT showed that in our patients morphological return to normal was the exception. Patients with head and brain injuries should be examined by MRT during the course of their illness. The use of special sequences, such as gradient-echo sequences for the diagnosis of haemorrhagic contusions, is indicated. CT should be retained for evaluating bone injury and cerebral damage during the acute stage.

Adult↗
PubMed1989

[The value of x-ray signs in the follow-up of Crohn's disease].

The value of various radiological signs in the follow-up of Crohn's disease has been studied in 175 radiological examinations of the gastro-intestinal tract in 39 patients. Changes in the significant radiological signs in Crohn's disease, such as spicula, cobblestone pattern, stenoses and fistulas, provide information on the development of the disease and on the effect of treatment. The signs have a prognostic significance only insofar as their persistence excludes any improvement. Radiological follow-up is particularly useful for spotting complications and for the early recognition of recurrences.

Adolescent↗
PubMed1989

[Digital subtraction angiography of the hand].

The report deals with 66 digital subtraction angiograms of the hand performed on 61 patients; 60 were carried out by direct puncture of the brachial artery and six by trans-femoral catheterisation. In 39 patients a peripheral organic vascular abnormality was demonstrated (usually arterial sclerosis, thromboangiitis obliterans or collagenosis). Purely functional flow abnormalities were found in 17 patients. In 15 patients an organic abnormality was demonstrated, but its type could not be identified. Intra-arterial DSA is able to differentiate between functional abnormalities of blood flow and peripheral organic vascular abnormalities.

Adolescent↗
PubMed1989

[The heart ventricle wall in digital subtraction angiocardiography].

Digital subtraction angiography, using a time interval difference mode in relation to a suitable mask, provides a means of showing the myocardium in every phase of the cardiac cycle. The endo- and peri-cardial contours and the thickness of the myocardium in both ventricles can be demonstrated accurately. Artifacts are usually due to displacements of the heart or to tachycardia.

Angiocardiography↗
PubMed1989

[Relaxation time measurements in the differential magnetic resonance tomographic diagnosis of liver tumors].

T1-weighted IR sequences and T2-weighted SE sequences and combined SE/IR sequences were carried out in 62 patients with malignant and 50 patients with benign liver tumours and the T1 and T2 relaxation times were measured. IR sequences proved the most sensitive method for imaging focal liver lesions and providing good contrast between liver tissue and tumour. For lesions above 1.5 cm, quantitative MRI achieved an accuracy better than 95% in distinguishing between haemangiomas and malignant lesions. Routine quantitative measurements of relaxation times were sufficiently reproducible for clinical use.

Carcinoma, Hepatocellular↗
PubMed1989

[Follow-up of portocaval anastomoses using DSA].

In 37 patients with porto-caval shunts, the operation site was examined on 40 occasions by i.a. DSA (25 Warren shunts, four Linton shunts and eight Drapanas shunts). The problem of shunt persistence could be resolved on 39 occasions. Only once was it not possible to evaluate the shunt because of artifacts. Artifacts caused by breathing, peristalsis and low contrast in the portal-venous segment did not prove serious problems in evaluating shunt patency. Intra-arterial DSA provides a comprehensive demonstration of the portal venous vascular territory and permits evaluation of post-operative haemodynamics.

Adult↗
PubMed1988

[Long-term course of chronic polyarthritis under basic drug therapy].

A retrospective analysis was undertaken of 31 patients with classical or proven chronic rheumatoid arthritis (CRA) who had been on a regimen of basic medication (gold salts, D-penicillamine, chloroquine, azathioprine--alone or in combination). Disease activity was checked by clinical, biochemical, immunological and radiological criteria. The laboratory results could not be altered by the basal medication and there was no relationship with the clinical and radiological findings, except for immunological results. None the less, the clinical symptoms improved under basal medication, even though the functional index got worse. The degree of joint destruction at the beginning and end of the observation period was markedly higher for seropositive than sero-negative cases, without significant differences in the rate of progression of joint destruction. Thus, definite long-term remission was achieved regarding the inflammatory changes but not the progressing joint destruction.

Adult↗
PubMed1988

[Color Doppler sonography: non-invasive potential for morphologic and functional vascular diagnosis].

A linear array colour Doppler sonographic method was used experimentally on a vessel phantom in order to determine flow velocity, and the results were compared with DSA. Both the Doppler and the DSA method showed very good agreement with true velocities (r greater than 0.97). The Doppler method underestimated flow velocity. Early clinical results indicate that colour Doppler sonography is able, at the same time, to demonstrate morphology and function (direction flow and flow velocity) of significant vascular abnormalities by a non-invasive method.

Blood Flow Velocity↗