PubMed Health⌕ Search

Biomedical subjects

T Hirose

Publications and source records attributed to T Hirose.

At least 829 records · Page 46Linked to original sources
PubMed1980

[Immunofluorescent analysis of intracellular localization of estrogen and progesterone in normal human endometrial cells (author's transl)].

Intracellular localization and the heat-dependent redistribution of estrogen and progesterone in human endometrial cells have been investigated by a fluorescent steroid-antibody technique. The dispersed endometrial cells were incubated with 5 X 10(-8) M estradiol-17 beta and progesterone in TC medium 199 containing 10% calf serum for 1--3 hr at 4 degrees C or 37 degrees C. An indirect immunofluorescent technique using FITC-labeled anti-rabbit IgG and steroid antibodies raised from rabbits immunizing with estradiol-6-oxime-BSA and progesterone-3-oxime-BSA was applied to the smear specimens. In normal endometrial cells in both proliferative and secretory stages, specific fluorescences to estradiol and progesterone were generally observed in the cytoplasm after incubation with the steroids at 4 degrees C for 1 hr. When these cells were incubated with the steroids at 37 degrees C for 1 hr, cytoplasmic and predominant nuclear fluorescences were detected, whereas the 3 hr-incubation at 37 degrees C resulted in disappearance of cytoplasmic fluorescence, remaining nuclear fluorescence alone. These fluorescences were remarkedly eliminated when endometrial cells were incubated with diethylstilbestrol and R-5020 prior to the incubation with estradiol and progesterone, respectively. These results indicate that the fluorescent steroid-antibody technique used in this study enables us to visualize subcellular localization of estradiol and progesterone possibly bound to receptors in each endometrial cell.

Endometrium↗
PubMed1980

[Cellular heterogeneity of steroid reactivity in human endometrial carcinoma: an approach by an immunofluorescent steroid-antibody technique (author's transl)].

The dispersed cancer cells obtained from biopsy or surgical specimens of 12 patients with endometrial carcinoma were incubated with 5 x 10(-8) M estradiol-17 beta or progesterone in TC medium 199 containing 10% calf serum at 37 degrees C for 1 hr. The immunofluorescent steroid-antibody technique using FITC-labeled anti-rabbit IgG and steroid antibodies raised from rabbits immunized with estradiol-6-oxime-BSA and progesterone-3-oxime-BSA was applied to the smear specimens and freeze-dry sections that had been incubated with these steroids. In 6 cases, there was a mixed cell-population with or without nuclear fluorescence to estradiol and progesterone. The cellular heterogeneity with respect to nuclear fluorescence was confirmed by the immunofluorescent technique applied to the freeze-dry sections. Two cases showed weak cytoplasmic fluorescence to estradiol and progesterone, but nuclear fluorescence was negative. In 3 cases, there was no immunoreactivity to these steroids. One case exhibited the similar distributional patterns of cytoplasmic and nuclear fluorescence to those of normal endometrial cells. These results indicated that in endometrial cancer, there were some impairments of subcellular steroid dynamics in comparison with those of normal cells and that cellular heterogeneity of the steroid reactivity might exist in some cases.

Adult↗
PubMed1980

Retinal functions in snowflake degeneration.

Retinal functions were studied in 7 patients with snowflake degeneration and correlated with fundus appearance. Despite the presence of vitreous turbidity, scotomatic glare sensitivity was slightly elevated in only one case. Retinal functions were affected more extensively in the late stages of the disease; however, abnormal retinal functions were also detected in some patients who showed only slight fundus changes on ophthalmoscopic examination. Kinetic perimetry showed peripheral defects which were more pronounced in the upper field. Flicker perimetry revealed abnormalities that kinetic perimetry could not detect. Dark-adaptation tests showed elevated rod thresholds except during the early stage of the disease. In all patients, the scotopic b-wave of the electroretinogram elicited by dim white light was low in amplitude. The photopic b-wave and the photopic flicker responses showed decreased amplitudes in some patients. The scotopic b-wave, normally recorded as a second positive peak when deep red light is used, almost disappeared, leaving only a small, single positive peak, photopic in nature, in the late stages. However, no patient had a nonrecordable electroretinogram. The electro-oculographic light peak/dark trough ratio was abnormal in only a few patients. These results indicate that in snowflake degeneration, functional abnormalities of the retina are milder than those in other progressive primary retinal degenerations typically represented by retinitis pigmentosa.

Adolescent↗
PubMed1979

Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage phi chi 174 have been synthesized by the phosphotriester method. The oligomers, 11, 14, and 17 bases long, are complementary to the region of the DNA which accounts for the am-3 point mutation. When hybridized to am-3 DNA, the oligonucleotides form duplexes with a single base pair mismatch. The thermal stability of the duplexes formed between wt and am-3 DNAs has been measured. The am-3 DNA:oligomer duplexes dissociate at a temperature about 10 degrees C lower than the corresponding wt DNA:oligomer duplexes. This dramatic decrease in thermal stability due to a single mismatch makes it possible to eliminate the formation of the mismatched duplexes by the appropriate choice of hybridization temperature. These results are discussed with respect to the use of oligonucleotides as probes for the isolation of specific cloned DNA sequences.

Bacteriophage phi X 174↗
PubMed1979

Thorium dioxide granuloma of the neck with resultant fatal hemorrhage.

A 46-year-old man had a granuloma in the neck that was caused by extravasation of thorium dioxide (Thorotrast) by an angiographic procedure performed about 30 years previously. His chief complaints were dysphagia and dyspnea with mild hoarseness. Parital resection of the tumor was performed, but his symptoms were not ameliorated. The immediate postoperative course was unfavorable. The patient died four months after the operation from massive hemorrhages from the right common carotid artery.

Carotid Arteries↗
PubMed1979

Electrodiagnosis of compression of individual nerve roots of the cauda equina.

Segmental electrodiagnosis of compression of individual nerve roots of the cauda equina has been attempted in 45 patients, including cases of disc hernia, spondylsis and spondylolisthesis. The needle electrode was inserted into the nerve root either just lateral to the intervetebral foramen or through the posterior sacral foramen. The recordings made on stimulating a single nerve root were somatosensory evoked potential, the cauda equina action potential and the M and H waves. Mixed spinal nerve root action potentials were also recorded by stimulating the sciatic, peroneal and tibial nerves. It was shown that a diagnosis could be made from the somatosensory evoked potential, the H wave and root pain reproduction, and also the diagnosis of a subclinical compression involvement. In other words, the neurophysiological state of dysfunction of individual roots in each aspect of compression can be expressed.

Action Potentials↗
PubMed1979

Foveal hypoplasia and bilateral 360-degree peripheral retinal rosettes.

A 24-year-old woman had bilateral pendular nystagmus, foveal hypoplasia, 360-degree peripheral retinal rosettes, and hypoplastic ora serrata of nongenetic origin. Kinetic perimetry revealed a generalized constriction of the field. Flicker perimetry revealed general depression of the critical flicker frequencies in the entire field. A slight increase of the threshold for light sensitivity after dark adaptation was noted from 10 to 30 degrees. Haidinger brush phenomenon was abnormal. Color vision was normal. The electroretinogram revealed mild abnormalities in the photopic flicker response. The visual-evoked response, recorded by local macular stimulation, was abnormal. The relationship of foveal hypoplasia to peripheral retinal rosettes in this case may have been caused by an intercurrent infection during the late stages of embryogenesis.

Adult↗
PubMed1979

Hereditary progressive arthro-ophthalmopathy of Stickler.

The ocular histopathologic findings in three patients with the Stickler syndrome from two families included the following: total retinal detachment with marked folding, disorganization of the retina, and a preretinal membrane. The progression of the fundus lesions was followed up in two patients during the course of 30 and 24 years. Many cases variously reported as Wagner's disease, familial retinal detachment, hyaloideoretinopathy with cleft palate, and the Pierre Robin syndrome probably were the Stickler syndrome.

Adolescent↗
PubMed1979

Expression in Escherichia coli of chemically synthesized genes for human insulin.

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. Complete purification of the A chain and partial purification of the B chain were achieved. These products were mixed, reduced, and reoxidized. The presence of insulin was detected by radioimmunoassay.

Amino Acids↗
PubMed1979

Secretion of aldosterone in response to histamine in hypophysectomized-nephrectomized dogs.

In hypophysectomized-nephrectomized dogs after intravenous injection of histamine, a marked increase was observed in the rate of secretion of aldosterone, although it was smaller than that in intact dogs. Hypophysectomy plus bilateral nephrectomy greatly impaired the secretion of corticosterone and cortisol in the dog in response to histamine. However, a small yet significant increase in corticosterone and cortisol secretion was observed in hypophysectomized-nephrectomized dogs after intravenous injection of histamine. Additional experiments showed that plasma concentrations of potassium and sodium in hypophysectomized-nephrectomized dogs remained unchanged after intravenous injection of histamine. These results suggest that histamine stimulates aldosterone secretion in the dog partly by a direct effect on the adrenal cortical cells, whereas the effect of histamine on corticosterone and cortisol secretion is mediated mainly, but not totally, by pituitary release of ACTH.

Adrenocorticotropic Hormone↗
PubMed1979

Effects of prostaglandin E2 and dibutyryl cyclic AMP on the histamine-induced production of cortisol and corticosterone in isolated canine adrenal cells.

The steroidogenic effect of histamine in isolated adrenocortical cells of the dog was investigated in the presence of prostaglandin E2 (PGE2) and/or dibutyryl cyclic AMP (dbcAMP) in the medium. The effect of histamine, in combination with PGE2 was less than their total individual effects in the production of cortisol, but not ofcorticosterone. With dbcAMP the effect was just equal to them. However, the combination of histamine, PGE2 and dbcAMP showed an increase twice that of their total individual effects in the production of both steroids. These results indicate that, in the dog, histamine, PGE2 and dbcAMP may act synergistically in the adrenocortical cells.

Adrenal Glands↗
PubMed1979

Pigmentary paravenous chorioretinal degeneration: fundus appearance and retinal functions.

A case of pigmentary paravenous chorioretinal degeneration initially diagnosed as retinitis pigmentosa is presented. The focal macular visual evoked response (VER) was normal. Both rod and cone components in the electroretinogram showed reduced amplitude with normal peak time. The psychophysical threshold in the dark adaptation was nearly normal in some parts of the retina and extremely elevated in other parts of the retina. Fundus appearance and retinal function studies suggest that the impaired area in the retina and choroid is localized in this disease.

Adult↗