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T Itoshima

Publications and source records attributed to T Itoshima.

At least 73 records · Page 4Linked to original sources
PubMed1982

Peritoneoscopic study on rat liver cell necrosis induced by carbon tetrachloride and allyl formate.

The surface changes of acute centrolobular and perilobular necrosis were studied in rat liver using peritoneoscopy. Centrolobular necrosis as induced by carbon tetrachloride and perilobular necrosis by allyl formate. Vessels observed on the control rat liver surface were identified as terminal hepatic veins by irrigation of the hepatic vein with a barium solution. Polygonal whitish markings were observed on the liver surface 48 hours after carbon tetrachloride administration. Terminal hepatic veins were regularly distributed throughout the central portions of the whitish areas. Reddish spots about 0.1 mm in diameter were scattered about the terminal hepatic veins. The reddish spots and whitish areas corresponded to the histological findings of central liver cell necrosis and infiltration by Kupffer and white blood cells. Six hours after allyl formate administration, round reddish spots about 0.3 mm in diameter were regularly distributed on the liver surface among the terminal hepatic veins. These spots corresponded to the histological findings of liver cell necrosis around the portal area. In summary, acute centrolobular and perilobular liver cell necrosis were newly observed as reddish spots by peritoneoscopy.

Animals↗
PubMed1982

Enhanced visualization of the portal vein system in superior mesenteric arterial portography using prostaglandin E1.

The portal vein system was clearly visualized in superior mesenteric arterial portography using prostaglandin E1. Angiographic examination was performed in 68 patients with various liver diseases during the 2 year period from 1980 to 1981. Twenty microgram of prostaglandin E1 was injected into the superior mesenteric artery 30 seconds before injection of 60 ml of contrast medium. The main portal vein was visualized in all of 68 cases. A high rate of success for visualization of the intrahepatic portal vein system by prostaglandin E1 was achieved. The first branches of the intrahepatic portal vein were visualized in 100% of the cases, the second branches in 82%, the third branches in 44%, and the fourth branches in 4% in the right portal vein system. In the left portal vein system, the first branches were visualized in 87%, the second branches in 41%, and the third branches in 3% of the cases. The intrahepatic portal vein system was more clearly visualized in females than in males (P less than 0.05). This procedure is simple, safe and useful for clear visualization of the portal vein system.

Alprostadil↗
PubMed1982

Percutaneous transhepatic portal catheterization-modification of Chiba method and portal vein pressure in liver diseases.

Percutaneous transhepatic portal catheterization was performed in 68 cases of liver diseases in the 2 year period from 1978 to 1980. The Chiba University method was modified. Portal vein catheterization was successful in 61 cases (90%). Selective splenic vein catheterization was successful in 55 of the 61 cases (90%) and selective superior mesenteric vein catheterization in 59 cases (97%). The liver was punctured an average of 4.6 times in order to successfully insert the catheter into the main portal vein, and the number of punctures was less than 10 in 57 of the 61 cases (93%). The portal vein pressure was 310+/-67 mm H2O in idiopathic portal hypertension (8 cases), 290+/-83 in liver cirrhosis (33 cases), 193+/-71 in chronic hepatitis (7 cases) and 166+/-50 in fatty liver (4 cases). Portal vein pressure rose from 205+/-75 to 380+/-55 mm H2O in 11 cases after forced Valsalva maneuver. No major complications were encountered.

Catheterization↗
PubMed1981

Kupffer cell hyperplasia in liver diseases. Demonstration by scanning electron microscopy of biopsy samples.

Kupffer cells were observed in liver biopsy tissues of 9 cases of liver diseases by scanning electron microscopy to prove Kupffer cell proliferation numerically. Kupffer cell count per 0.01 mm2 of cracked surface of liver lobule was 1.2 +/- 0.3 in the convalescent stage of a mild acute hepatitis case and 1.2 +/- 0.1 in a chronic persistent hepatitis case with slight inflammation. Whereas it was increased to 2.4-5.5 (P less than 0.01) in the convalescent stage of moderate to severe acute hepatitis cases, 1.8 +/- 0.1 (p less than 0.05) in a chronic active hepatitis case, 2.5 +/- 0.3 (p less than 0.001) in an alcoholic portal fibrosis case and 2.2 +/- 0.4 (p less than 0.001) in a liver cirrhosis case. Kupffer cell count per mm3 of liver lobule was estimated roughly 3,500 in the convalescent stage of a mild acute hepatitis case and in a mild chronic persistent hepatitis case and 7,000 to 16,000 in the convalescent stage of moderate to severe acute hepatitis cases.

Acute Disease↗
PubMed1981

Scanning electron microscopy of rat degenerated hepatocytes after carbon tetrachloride intoxication.

Intracellular structures of hepatocytes were studied in rats intoxicated with carbon tetrachloride (CCL4) by scanning electron microscopy (SEM). Liver tissues were treated successively by conductive staining, critical point drying and fracturing with a scalpel. Fractured hepatocytes were mainly observed in the specimens 12 hours after CCL4 administration, Hepatocytes near the portal tract showed a normal dense cytoplasm. Swollen foamy hepatocytes were remarkable in the intermediate zone. They had numerous vesicles, 0.6-2.5 micrometer in diameter, which correspond to dilated cisternae of rough endoplasmic reticula (RER) observed by transmission electron microscopy. The vesicles were separated by a thin rim of the cytoplasm and communicated with each other through a round window. Besides vesicles, the foamy hepatocytes had some vacuoles 3-10 micrometer in diameter, which were extremely dilated RER. The vacuoles had many windows and communicated with the vesicles. Hepatocytes with dense cytoplasm and many vacuoles were distributed widely in the lobule. They were intermingled with the vesiculated hepatocytes inthe intermediate zone. Their vacuoles were 1.5-3.0 micrometer in diameter and some vacuoles communicated with each other. The vacuole had a well-developed wall with 0.1 micrometer diamter pores. The pores were sometimes arranged in line. Hepatocytes near the central vein showed necrotic changes. Most membraneous structures were disrupted except for fat droplets and the nucleus. The nucleoplasm was looser and nuclear pores disappeared. In summary, SEM demonstrated three-dimensionally organelle changes of hepatocytes induced by CCL4 administration.

Animals↗
PubMed1980

Scanning electron microscopy of the rat bile canalicular-ductular junction.

Scanning electron microscopy revealed two types of bile canalicular-ductular junctions in rats. 1. Several bile canaliculi converged to enter the terminal bile ductule by direct continuity, forming an ampulla just before the junction. The ampulla was about 2.5x6 micrometers, whereas the canalicular diameter was about 1.5 micrometer just before the ampulla and the ductular diameter was 1.2--4.0 micrometers. The ampulla was mainly walled by several hepatocytes and partly by a few ductular cells. The hepatocytes abutted on the bile ductular cells. 2. Bile canaliculi connected directly to the bile ductule as a side branch without forming the ampullary dilation. In this case, a part of the bile ductular canal was formed by the hepatocytes at the junction of the bile canaliculus with the bile ductule. This study confirmed the light microscopy observation of Lettule and McIndoe.

Animals↗
PubMed1979

Scanning electron microscopy of the parimarginal cavernous sinus plexus of the human spleen.

The perifolicular region of the human spleen was studied under the scanning electron microscope. The marginal zone 10 to 50 micron in width was located around the white pulp, and was composed of concentrically arranged reticulum cell networks and intervening lymphocytes, red blood cells and macrophages. The cavernous sinuses 10-150 micron in width were situated between the marginal zone and the red pulp, and were lined by thin, flat endothelia. The perimarginal cavernous sinuses communicated with one another through narrow canals and formed a plexus. Transmural passages of lymphocytes were noted on the side of the white pulp. The perimarginal cavernous sinus plexus was also in communication with the interspaces between reticulum cells in the marginal zone. The human perimarginal cavernous sinus plexus appears to correspond to the marginal sinus in rat and rabbit spleens.

Aged↗
PubMed1978

Cleaning of scanning electron microscope specimens by puncture perfusion.

Puncture perfusion method was described for elimination of blood and tissue fluid in the scanning electron microscope specimens. It was also demonstrated that the puncture perfusion is of excellent use in cleaning the small samples including the human biopsy samples, which are hardly washed by the vascular cannulation.

Cytological Techniques↗
PubMed1977

Scanning electron microscopy of the bile ductule.

Scanning electron microscopy of a liver biopsy specimen from a patient 6 months after the onset of acute hepatitis revealed a normal appearing bile ductule. The bile ductule was 13 micron in outer diameter and 1.5-2.7 micron in inner diameter. The ductular lumen was surrounded by two ductular cells and ampullary dilated at the canalicular side. In the lumen, intracytoplasmic diverticles were observed with an orifice diameter of 0.9 micron. About 15 microvilli 0.4-0.6 micron in length and 0.1 micron in thickness were observed on 1 micron2 of luminal surface. The number of microvilli was calculated to be approximately 1,500 per ductular cell. A single cilium 0.15 micron in diameter at the base and 0.10 micron at the trunk, and 7-15 micron in length was found on each ductular cell. The cilium arose from a recess at the canalicular side of the ductular cell, and the free-end was on the bile-duct side. The cilia seemed to play an important role in bile flow to the duct. Sometimes between the ductular cells at the edge of the basal portion a cell was intercalated with thin processes. Such thin processes were not observed on ductular cells.

Adult↗
PubMed1977

Modified tannin-osmium conductive staining method for non-coated scanning electron microscope specimens. Its application to microdissection scanning electron microscopy of the spleen.

Supplement of guanidine hydrochloride to the original tannic acid solution of MURAKAMI (1973) eliminated, without debilitating the conductivity effect, the vexatious treatment with different amino-acids and sucrose in the previous method (MURAKAMI, 1974). The procedures and advantages of this simplified modification are described using human spleen samples; attempt was made to elucidate the structure of arterial terminals by dissecting the specimens under the scanning electron microscope.

Dissection↗
PubMed1976

A scanning electron microscopy of subcellular structures of the human hepatic cell.

Human liver specimens obtained by needle biopsy were extensively osmicated by the revised tannin-osmium method of MURAKAMI (1974) and cracked in air after critical point drying, to be observed in the scanning electron microscope. The fracture tended to occur along the membranous elements and exposed beautifully fractured faces of the hepatic cell. The nucleus of the hepatic cell was spherical. The nuclear pores were demonstrated as small pits 0.1 mu in diameter and their total occurrence in one nucleus was estimated to be 2,100. In the spongy framework resembling the endoplasmic reticulum, oval-shaped mitochondria and multivesicular tufts of the Golgi complex were observed. The nucleolus of the hepatic cell nucleus was noticed as a highlighted mass in the cloudy nucleoplasm.

Cell Nucleus↗
PubMed1976

Peritoneoscopic diagnosis of liver surface--using a new code number system--.

A new peritoneoscopic classification of liver surface using a three digit code was developed. The first digit represents the lobular change from normal structure to nodules. The second digit represents the size of the nodule and the internodular distance. The third digit represents the pathological findings, such as inflammation, degeneration, necrosis or regeneration. Furthermore, peritoneoscopic findings of necrosis of lobular size around the portal vein and patchy markings of the liver were presented. These findings were the most prominent in the course of hepatitis progression to cirrhosis.

Adult↗