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T K Borisova

Publications and source records attributed to T K Borisova.

7 recordsLinked to original sources
PubMed1999

Role of different lymphocyte subpopulations in the formation of non-specific immunoglobulins induced by antigen injection.

The formation of antibody and non-specific immunoglobulin under the influence of T-dependent (TD) and type 2 T-independent (TI-2) antigens in mice of two congenic strains CBA (Lyb5-, Lyb5+) and CBA/N (Lyb5-) was studied. TD antigens induced in mice of both strains not only the appearance of antibody-forming cells (AFC), but also a great increase in the number of cells producing non-specific immunoglobulins (nIFC). TI-2 antigens induced the AFC and antigen-dependent nIFC formation in CBA mice only. It is concluded that during immune response to TI-2 antigens not only the AFC appearance but the increase in nIFC formation (polyclonal activation) is due mainly to the mature Lyb5+ B cells.

Animals↗
PubMed1990

[The dynamics of Treponema-specific blood immunoglobulins in early forms of syphilis].

The study has involved 73 patients with syphilis, 31 female and 42 male ones, aged 18-42. Four of these suffered from primary seronegative, 14 from primary seropositive, 21 from secondary fresh, 22 from secondary recurrent, and 12 from early latent seropositive syphilis. Peripheral blood sera were under study. Treponema-specific antibodies of the IgM and IgG classes were titered by enzyme immunoassay. The detected changes in Treponema-specific immunoglobulinemia are in good correlation with clinical staged pattern of syphilis and antiinfectious immunity status.

Adolescent↗
PubMed1987

Ir gene influence on DNP-specific B-memory-cell formation.

It is well known that Ir genes control the antibody production. To investigate whether they also influence B-memory-cell generation, CBA mice (nonresponders) were primed with dinitrophenyl-poly-(L-tyr,L-glu)-poly-(D,L-ala)-poly-(L-lys)-DNP-TGAL conjugate. At the same time animals were injected with ovalbumin (OVA) to activate OVA-specific T helpers. Two to four weeks later animals were challenged with DNP-OVA conjugate and the number of IgG-producing B cells was determined. The data presented indicate that carrier-specific MHC-restricted T helpers are not required for B-memory-cell generation. It is concluded that the defect of IgG response to DNP-TGAL in CBA mice is caused by a block in the maturation of memory cells to antibody-producing cells.

Animals↗
PubMedDate not supplied

[Human monoclonal antibodies to synthetic viral peptides].

Cells producing human antibodies to synthetic viral peptides MH-24 (corresponding to 302-325 residues of HIV-1 gp120) and p107 (main peptide of EBV antigen EBNA-1) were fused with human x mouse heteromyeloma B6B11. The efficacy of specific hybridization was 40%. Hybrid cells H1F2 (MN-specific) and HF4 (p107-specific) were cloned by the limiting dilutions method. Monoclonal antibodies (MAb) were tested in enzyme immunoassay on a panel of synthetic peptides. All resultant MAbs were polyspecific for tested synthetic peptides. The data permit a conclusion that previously detected polyspecificity of human cultural antibodies to virus peptides is due to the true polyspecificity of antigen-binding centers of MAb, but not to the presence of strange cells in the culture.

Animals↗
PubMedDate not supplied

[Role of cytokines in immune response to T-independent antigens, type 2].

The influence of human and mouse cytokines on the induction of immune response to model T-independent (TI) antigens of type 2--DNP-dextran and DNP-ficoll--in the culture of mouse spleen cells was studied. For the first time this study revealed that the action of definite T-cell factors not only induced the polyclonal stimulation of B lymphocytes, but also the increased synthesis of specific antibodies, as well as switched over antibody isotypes from IgM to IgG. This confirmed the suggestion that T cells took part (directly or indirectly) in the regulation of immune response to so-called TI antigens. These results widened our knowledge on the mechanisms of the development of humoral immune response to TI antigens of type 2.

Animals↗
PubMedDate not supplied

[Isolation of human B-cells, specific for viral peptides, using magnetic spheres].

A method for isolation of human B cells specific to viral peptides using magnetic beads has been developed. The method was used for the isolation from human tonsils of B cells specific to HIV-1 gp 120 MN-24 peptide (residues 302-322) and to Epstein-Barr virus 107 EBNA-1 peptide. The cells selected with beads were transformed by Epstein-Barr virus. The resultant cell cultures produce human immunoglobulin antibodies to HIV-1 MN-24 and to Epstein-Barr EBNA-1 p107 peptides.

Antigens, Viral↗