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T L Rothwell

Publications and source records attributed to T L Rothwell.

At least 73 records · Page 4Linked to original sources
PubMed1977

Failure to demonstrate involvement of prostaglandins in the immune expulsion of Trichostrongylus colubriformis from the intestine of guinea pigs.

The intraduodenal injection of synthetic prostaglandins did not lead to the expulsion of Trichostrongylus colubriformis from the intestine of guinea pigs. Treatment of immune guinea pigs with aspirin and indomethacin failed to inhibit the expulsion of a challenge infection with this nematode. These results suggest that in this infection, unlike Nippostrongylus brasiliensis infection in the rat, prostaglandins do not play an important role in the immune expulsion of the parasite from the intestine.

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PubMed1976

Basophil leucocytes in cutaneous hypersensitivity reactions in nematode-infected guinea-pigs.

Cutaneous hypersensitivity reactions were elicited in guinea-pigs infected with the parasitic nematode Trichostrongylus colubriformis by injecting them with a crude extract of T. colubriformis fourth-stage larvae. The reaction was characterized by early oedema and pronounced cellular infiltration, initially with neutrophils but later with mononuclear cells, basophils and variable numbers of eosinophils. Because basophils have been implicated as effector cells in the protective immune response of guinea-pigs to this nematode, the capacity to elicit a basophil-rich cellular infiltrate in infected animals might be a useful assay for T. colubriformis protective antigen(s).

Animals↗
PubMed1975

Studies of the responses of basophil and eosinophil leucocytes and mast cells to the nematode Trichostrongylus colubriformis. I. Observations during the expulsion of first and second infections by guinea-pigs.

During expulsion by guniea-pigs of both first and second infections with the intestinal nematode parasite Trichostrongylus colubriformis, increased numbers of basophils and eosinophils were present in the bone marrow and blood. In addition, increased numbers of basophils, mast cells and eosinophils, many possessing reduced numbers of cytoplasmic granules, accumulated at the sites of infection. Blood eosinophil counts, however, did not show significant changes during the early part of a first infection.

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PubMed1975

Studies of the responses of basophil and eosinophil leucocytes and mast cells to the nematode Trichostrongylus colubriformis. II. Changes in cell numbers following infection of thymectomised and adoptively or passively immunised guinea-pigs.

The role of the immune response in the generation of the basophilia and eosinophilia found during expulsion of the intestinal nematode parasite, Trichostrongylus colubriformis, by guinea-pigs was investigated by studying cell numbers in animals whose immune responsiveness had been modified by thymectomy and adoptive or passive immunisation. Basophilia, but not eosinophilia, was depressed in thymectomised guinea-pigs. Bone marrow basophil numbers were significantly increased in T. colubriformis-infected guinea-pigs following the infection of mesenteric lymph-node cells from both normal and T. colubriformis-immune syngeneic donors. Bone marrow basophil counts were also increased following the injection of immune lymph-node cells into uninfected recipients. Small intestine eosinophil numbers in adoptively immunised guinea-pigs showed a pronounced increase following infection with T. colubriformis. A smaller increase followed infection of passively immunised guinea-pigs. These results, and other work with this system, suggest that basophilia and eosinophilia during T. colubriformis infection, although associated with the immune response, might not be fully explained as direct consequences of the interaction of parasitic antigens and sensitised lymphocytes or antibodies.

Animals↗
PubMed1975

Studies of the responses of basophil and eosinophil leucocytes and mast cells to the nematode Trichostrongylus colubriformis. Ultrastructural changes in basophils and eosinophils at the site of infection.

Basophil and eosinophil leucocytes infiltrate the small intestinal lamina propria of guinea-pigs infected with the nematode Trichostrongylus colubriformis. By comparing the morphology of both cell types at the site of infection with bone marrow and buffy coat cells, it was found that, after entering the lamina propria, basophils developed an electron lucent halo beneath the granule-limiting membrane while the characteristic orderly periodicity of the granules changed to a fibrillar or amorphous appearance. The granules also tended to coalesce but remained within the cell. Approximately half the eosinophils at the site of infection developed deficiencies in the amorphous outer matrix of their granules but showed no obvious change in the central electron-dense cores.

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