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Biomedical subjects

T M Murad

Publications and source records attributed to T M Murad.

At least 55 records · Page 3Linked to original sources
PubMed1978

Sclerosing cholangitis associated with ulcerative colitis. Light and electron microscopy studies.

The light and electron microscopy findings of liver biopsies from four patients with sclerosing cholangitis and ulcerative colitis are described. The main histologic feature was mesenchymal proliferation involving phagocytic, fibroblastic and immunocytic cells suggestive of an immunologic type of liver injury which may attack bile duct epithelial cells. Other histologic features, included the presence of myelin fibers in both hepatocytes and bile duct epithelial cells and an abundant amount of lysosome-like structures. The significance of these findings is discussed.

Adult↗
PubMed1977

Malignant and benign papillary lesions of the breast.

This presentation describes criteria that we found most helpful in classifying the various proliferative changes of the breast and in separating papillary carcinoma from other benign papillary lesions. The criteria we used are easily understood and extremely reproducible. The true incidence of benign proliferative changes in fibrocystic disease and the significance of these lesions in later development of cancer were determined by studying 200 randomly selected cases of fibrocystic disease seen at least 10 years earlier. Of the 200 cases of fibrocystic disease, 40 showed intraductal papillomatosis or terminal duct hyperplasia. None of the patients with benign proliferative lesions for whom we have complete follow-up data (up to 14 years) developed cancer. In order to determine the type of proliferating cells in papillary carcinoma and benign proliferative lesions, some of the recently encountered cases were studied by transmission and scanning electron microscopy and histochemistry. Our ultrastructural and histochemical study suggests that both epithelial and myoepithelial cells participate in papillary lesions of the breast.

Adult↗
PubMed1977

Evolution of reactive lymphadenopathy into lymphoma over a nine-year period. A clinicopathologic study.

Reported is a case of lymphoma which was preceded for nine years by an apparently reactive lymphadenopathy. Original slides of the multiple lymphoid tissue samples are reviewed, with emphasis on the gradually increasing numbers of mitoses, atypical histiocytes, and eosinophils, suggestive of malignant transformation. The scarcity of such cases in the literature prompts this report.

Adult↗
PubMed1977

Glucose-6-phosphate dehydrogenase isoenzymes in acinar epithelial cells separated from the mammary gland of the rat at intervals during the course of lactation.

Acinar epithelial cells were purifed from suspensions of cells from the lactating mammary glands of rats. As described previously, this purififaction was accomplished by sedimentation in an isokinetic gradient of Ficoll (polysucrose) in tissue culture medium, and the purity of the separated cells was established by electron microscopy and by histochemical markers. Isoenzymes of glucose-6-phosphate dehydrogenase were investigated at various intervals during lactation in separated populations of stromal and acinar cells. Acinar cells contained three- to eightfold more glucose-6-phosphate dehydrogenase activity than did stromal cells. The proportions of the respective isoenzymes varied during the course of lactation, and the observed changes were parallel in purified acinar cells and in the lactating mammary glands from which the cell suspensions were obtained. The availability of purified acinar cells in the investigation of interactions between hormones and cells from the mammary gland permits a greater degree of specificity than has been possible in the study of mammary cell suspensions which contain myoepithelial cells, duct cells, acinar cells, endothelial cells, fibroblasts, fat cells, mast cells, plasma cells, and blood cells.

Animals↗
PubMed1976

Separation of cells with histochemically demonstrable acid phosphatase activity from suspensions of cells from human prostatic carcinomas in an isokinetic gradient of Ficoll in tissue culture medium.

This report describes the separation of cells exhibiting histochemically demonstrable acid phosphatase from suspensions of cells obtained from human prostatic carcinomas by velocity sedimentation. In the unseparated suspensions of cells, 40.5 +/- 7.7% of nucleated cells contained histochemically evident acid phosphatase. After cells were separated by velocity sedimentation, 86.4 +/- 9.4% of the nucleated cells in the purest fractions exhibited histochemically demonstrable acid phosphatase activity. More than 95% of these cells excluded trypan blue. To our knowledge, this is the first report of a method for the separation of viable epithelial cells from human prostatic carcinomas.

Acid Phosphatase↗
PubMed1975

The pathologic behavior of primary vaginal carcinoma and its relationship to cervical cancer.

The surgical pathology files at the University of Alabama Medical Center for 1958 through 1973 contain records of 157 cases of vaginal carcinoma. Of the 141 patients on whom complete records are available, 37 had primary and 104 secondary vaginal carcinoma. Only 3 of the primary vaginal malignancies were adenocarcinoma; the rest were epidermoid. The microscopic appearance of these carcinomas was frequently lateral spreading or papillary, but in a few instances the growth pattern was submucosal. Prognosis appeared to be related to the stage of the disease. Vaginal carcinomas associated with cervical cancer clustered either within 1 year or 5 years after the therapeutic treatment. In this paper, the relationship between primary vaginal cancer and cervical cancer is discussed.

Adenocarcinoma↗
PubMed1975

Mithramycin in the treatment of systemic mastocytosis.

A patient had systemic mastocytosis of 16 years' duration, with urticaria pigmentosa, osteopetrosis with severe bone pain, mastocytosis of the bone marrow with myeloid metaplasia, gastrointestinal malabsorption, and Cushingold features associated with prolonged usage of corticosteroids. Treatment with a single dose of mithramycin resulted in an abrupt disappearance of the patient's generalized bone pain, but it had little effect on his histamine release-type symptomatology.

Adult↗
PubMed1974

Separation of acinar cells from lactating mammary gland using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

This report describes a procedure for the purification of acinar cells from the disaggregated lactating mammary gland of the rat. Following disaggregation of the 15 days postpartum mammary gland, two qualitatively different kinds of cells which exhibited alkaline phosphatase activity were identified. These two populations of cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium using a centrifugal force of 55g for 14 minutes. The larger of these two types of cells was found in a well-defined subpopulation of mammary cells, 97.4 +/- 0.8% of which lacked demonstrable adenosine triphosphatase activity by light microscopy. Ultrastructurally, these cells were identified as being almost exclusively acinar cells. The potential significance of this method is discussed.

Alkaline Phosphatase↗