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Biomedical subjects

T Miyamae

Publications and source records attributed to T Miyamae.

At least 91 records · Page 5Linked to original sources
PubMed1990

Comparative evaluation on mouse nasal immunogenicity of arylmethane-, xanthene-, quinone-imine-, and acridine-dye-inactivated Sendai virus vaccines.

Twenty-seven kinds of organic dye-inactivated Sendai virus vaccines were prepared by treatment in dark at 23 C for 2 months or more, and selected with the high HA titers as a guide. Their nasal immunogenicities were examined in mice by contact infection and immunofluorescent method, and the relative merits of the dye-inactivants were determined. The strongest protection was elicited with acriflavine-, auramine O-, eosin Y-, neutral red-, night blue-, patent blue V-, thymol blue-, uranin-, and xylene cyanol FF-treated vaccines. Middling protective efficacy was induced by use of erio green B-, malachite green-, methyl green-, proflavine-, pyronin B-, and thionin-inactivated vaccines. Dye-inactivated vaccines that resulted in the weakest protection were Bindschedler's green-, bromothymol blue-, erythrosin B-, ethyl violet-, gallein-, light green SF yellowish-, methyl violet-, new methylene blue N-, phenol red-, rhodamine 6G-, spirit blue- and victoria blue B-treated ones. Serum HI titers developed by nasal vaccination were variable, and rose still more in most vaccinated groups postexposure. Elicitation of the most effective nasal immunogenicity in dye-inactivated vaccines appeared to depend on selective modification of capsid protein or ribose in viral core with dyes possessing definite functions, despite the different molecular structures.

Animals↗
PubMed1990

Dose-dependent transplacental infection of murine encephalomyelitis virus GDVII in gravid uterus.

Intrauterine infection of murine encephalomyelitis virus GDVII was investigated by the indirect immunofluorescence method. Sixty-day-old pregnant mice were inoculated with 5 gradated doses of the virus ranging from 0.001 to 1.5 MLD50 via i.v. route on the 10th day of gestation. The mice exsanguinated on postinfection day 8 showed high incidences of viral antigen in the uterine walls, placentas, and fetal subcutaneous tissues with doses of 0.1-1.5 MLD50, regardless of the presence of maternal symptoms. Incidence of viral antigen-positive cells in fetal brains was high with a dose of 0.1 MLD50, but not by the other doses. However, the brains of the stillborn and newly-born mice derived from females infected by a dose of 0.5 MLD50 brought about still higher detection rates of viral antigen, as well as in the postpartum uteri. In effect, transplacental transmission of the virus was clearly demonstrated, and appeared to be dose-dependent.

Animals↗
PubMed1990

Heat production as a quantitative parameter for cell differentiation and cell function.

Heat production was measured in relation to cell differentiation and phagocytie function using cells of human monocyte-histiocyte cell line U937. U937 cells differentiated monocytic phagocytes when cultured with lymphokine. Heat production increased as result of differentiation and phagocytosis. An important finding was that the heat increase in differentiated cells and during phagocytosis was directly proportional to the concentration of lymphokine. This strongly suggested that heat production is a quantitative parameter not only for cell differentiation but also for phagocytic function. The measurement of heat produced by mammalian cells can therefore be used to quantitate the differentiation and function of cells.

Body Temperature Regulation↗
PubMed1990

Heat production due to intracellular killing activity.

Using Saccharomyces ceravisiae, Candida albicans and Stapylococcus aureus, heat production during phagocytosis was measured in U937 cells which are capable of differentiating to monocytic phagocytes. No increase in heat production of non-differentiated U937 was observed since they were not phagocytic cells. However after differentiation to monocytic phagocytes by lymphokine, U937 cells produced a remarkable amount of heat during phagocytosis. Although Ehrlich ascites tumor cells sensitized with antibody were capable of engulfing S. aureus, no increase in heat nor in superoxide anion production during phagocytosis was detected. It was also found that no heat increase occurred in neutrophils from a patient with chronic granulomatous disease (CGD). It can thus be concluded that the heat production during phagocytosis is due to the intercellular killing process of phagocytic cells.

Animals↗
PubMed1988

Heat production as a quantitative parameter of phagocytosis.

Microcalorimetry was applied to measure phagocytosis by human peripheral blood neutrophils and monocytes. Heat production was 9.1 +/- 2.6 microW by 1 X 10(6) unstimulated neutrophils and increased to 28.4 +/- 3.2 microW in association with phagocytosis. The increase in heat production was directly proportional to the number of Saccharomyces cerevisiae particles phagocytosed as well as to the concentration of opsonizing serum. No heat increase was observed in the absence of phagocytosis. An increase in heat production by monocytes was also observed in association with phagocytosis, but it was much less obvious than that by neutrophils. Heat production can thus be used as a quantitative measure of phagocytosis.

Calorimetry↗
PubMed1987

Visualization of distal splenorenal shunt by isotopic splenovenography.

Radioisotopic angiography of the splenic vein was performed in six patients following a distal splenorenal shunt (Warren procedure). Under echographic guidance, the spleen was punctured with a 22-gauge needle, and Tc-99m pertechnetate was injected into the splenic pulp. In five patients whose esophageal varices had remained atrophic, the splenic vein, the left renal vein, the inferior vena cava, and the heart were clearly visualized within 8 seconds. In one patient, in whom recurrence of esophageal varices had been recognized, the splenic vein was not imaged. The injected material coursed mainly upwards through collaterals.

Aged↗
PubMed1986

Heat production as a cell cycle monitoring parameter.

A microcalorimetric method was applied to define the cell cycle by measuring heat production of mouse breast cancer cell line, FM3A. FM3A cells, were synchronised at the Go phase and produced 13.0 mu Watts (W) per 1 X 10(6) cells. Although the number of cells in fresh medium remained unchanged during the following 24 hour, a dramatic increase of heat production was observed and maximum heat (46.2 mu W) was produced by the cells at 24 hours when the cell cycle was presumably at the G2 phase. At 26 hours, although cell number increased, heat production decreased. Since the cells were not treated in any manner, this microcalorimetric method of measuring the cell cycle by monitoring heat production can be a very useful tool.

Calorimetry↗
PubMed1986

Protective effects of nasal immunization in mice with various kinds of inactivated Sendai virus vaccines.

The immunoprophylactic effects of nasal vaccination with 13 different kinds of inactivated Sendai virus vaccines were compared by contact exposure to infector mice. Efficacies of the vaccines were evaluated on the basis of the presence of virus-infected cells by immunofluorescent examination of the entire respiratory tract, including the nasal mucosa. A single or double inoculations of B-propiolactone (0.5%)-vaccine promoted the infection in the respiratory tract, particularly in the nasal mucosa, whereas three inoculations of B-propiolactone (0.2%)-vaccine provided considerable protection throughout the respiratory tract with only slight development of serum HI titer. Formalin (0.1%)-vaccine and UV irradiated-vaccine strongly protected the nasal mucosa from infection, but did not sufficiently safeguard the lower respiratory tract even with three vaccinations despite adequate development of serum antibody. Nearly complete protection of the entire respiratory tract was induced with six to eight inoculations of a vaccine treated excessively with both UV rays and 1% formalin, without significant development of serum antibody. Out of eight thermal vaccines, five (inactivated at 23 C, 30 C, 37 C and 7 C, and 30 C and 7 C) provided strong protection against infection when inoculated three times. The others inactivated at higher temperatures (37 C, 50 C, or 60 C) were not so protective. High serum HI titers developed, on the whole, with the drop in the temperature required for inactivating the virus. In eight immune mouse groups in which infection was strongly suppressed in the entire respiratory tract, most of the mice harbored less than 50 viral antigen-positive cells in their nasal mucosa in the postexposure period. The number of the cells was assumed to be a useful criterion for evaluation of vaccine efficacy.

Administration, Intranasal↗
PubMed1986

[Radionuclide angiography (scintiphotosplenovenography) in the evaluation of splenorenal shunt patency].

Radionuclide angiography of the splenic vein (Scintiphotosplenovenography: SSV) was done in 7 patients following a distal splenorenal shunt (Warren procedure). Spleen was punctured with a 22 gauge needle under ultrasonographic guidance. One half ml of 99m TcO4- solution, which contained 10 mci of nuclide energy, was injected into the splenic parenchyma through the needle. Injected material was followed by scintillation camera. In five cases, whose esophageal varices had been endoscopically atrophic, the splenic vein, the left renal vein, the inferior vena cava and the heart were clearly viewed in a continuity within 8 seconds from the start of injection. In one case, whose varices had recurred soon after the operation, only the collaterals were delineated. Most of them seemed running upwards alongside the esophagus. In the remaining patient, whose varices had been atrophic, the splenic vein was faintly delineated within 8 seconds. However, most of the 99m TcO4- stayed at first in the spleen. Thereafter it went out into the collaterals, the lower intercostal vein and the hemiazygos vein. This method is less invasive than the conventional angiography. It can reveal not only the entire imaging of shunt flow but also the collaterals, if they exist. It seemed useful for the evaluation of shunt patency following distal splenorenal shunt.

Female↗
PubMed1985

Systemic dissemination of ethanolamine oleate after injection sclerotherapy for esophageal varices.

Sclerotherapy for esophageal varices was performed on six patients for a total of 16 injections. The sclerosant contained 5% ethanolamine oleate and technetium Tc 99m sodium pertechnetate solution at the volume ratio of 9:1. At each injection session 5 to 20 mL of this solution was injected into one varix. The distribution of the injected material was observed by a scintillation camera. Systemic dissemination of the sclerosant through the portal vein was demonstrated in five procedures, in which the injections were correctly intravenous. In three dogs 4 mL of 5% ethanolamine oleate made from oleic acids labeled with iodine 131 was injected into the gastric coronary vein. Most of the substance injected was washed out within five minutes, and systemic dissemination became evident.

Adult↗
PubMed1985

Prevention of bacterial contamination of water in dental units.

A study of the bacterial contamination of water in dental units was conducted in five dental practitioners' clinics and one university dental hospital in Tokyo. Various bacteria in numbers of 10(2)-10(5)/ml were detected in the water delivered from all 42 of the dental units examined, though very few bacteria were detected in the tap water upstream of the units. Most of the organisms isolated from the water were Gram-negative bacilli, predominantly Alcaligenes, Achromobacter and Pseudomonas spp. A new decontamination apparatus that could be incorporated into the pipe system of dental units was devised and its performance was evaluated in one unit. It effectively prevented bacterial contamination of the water.

Bacteria↗