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T Ninomiya

Publications and source records attributed to T Ninomiya.

At least 127 records · Page 7Linked to original sources
PubMed1988

[Attempted marrow rescue with cryopreserved circulating stem cells following high-dose chemotherapy].

Circulating hematopoietic stem cells were collected by three consecutive leukaphereses during post-chemotherapy expansion of the stem cell pool in a 3-year-old boy with advanced and therapy-resistant neuroblastoma. The cells were fractionated by discontinuous Percoll gradient centrifugation, frozen in a programmed freezer and then stored in liquid nitrogen. Following high-dose chemotherapy, the cells were thawed rapidly and re-infused into the patient. Early evidence of marrow recovery was first noted at day 13 and the times required to achieve a granulocyte count of greater than 0.5 x 10(9)/L and a platelet count of greater than 50 x 10(9)/L were 21 days and 30 days, respectively. This new marrow-rescue operation may have potential in cancer therapy as an alternative to bone marrow transplantation and further clinical investigation is warranted.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1988

[Autologous peripheral stem cell transplantation].

A number of recent reports have now shown that hematopoietic stem cells are present in the circulation, and these cells have the potential to restore complete hematopoiesis following marrow aplasia. Peripheral stem cells have been collected by continuous flow leukapheresis during periods of hematopoietic regeneration after 2 to 3 weeks of intensive chemotherapy. Following marrow ablative chemotherapy or chemoradiotherapy, the autologous peripheral stem cells are thawed and infused intravenously. When a sufficient stem cell dose was given, rapid neutrophil and platelet recovery has been confirmed, as compared with bone marrow transplantation. Acute leukemia, malignant lymphoma and solid tumors may benefit from this approach, and long-lasting remission or cure may result in a significant number of patients.

Acute Disease↗
PubMed1988

[Cryopreservation of circulating stem cells in large quantities].

We have collected peripheral blood mononuclear cells in large quantities by leukapheresis from children with malignant disorders. The cells were fractionated on discontinuous gradients of Percoll for enrichment of hematopoietic stem cells and reduction of sample volume. They were subsequently frozen in a programmed freezer and stored in liquid nitrogen. This procedure allows a reduced DMSO volume to be infused into patients and increases storage space. The recovery rates of progenitors evaluated by freeze-thaw analysis in small aliquots were 89% for granulocyte-macrophage colony-forming units (CFU-GM) and 106% for granulocyte, erythroid, macrophage, megakaryocyte colony-forming units (CFU-GEMM). Hence, we conclude that circulating stem cells can be collected and cryopreserved in large quantities without any loss of capacity. Further studies to evaluate the relevance of performing rescue surgery with these stem cells following marrow-ablative chemotherapy are underway.

Freezing↗
PubMed1987

Morphological changes of dorsal root ganglion cells in the process-forming period.

Rat DRG cells at early stages of development were observed to clarify the forming process from bipolar to pseudounipolar cells. Dissociated DRG cells in vivo and in vitro were observed by light and scanning electron microscopy. The percentage of cell types in the 13th prenatal to the 1-day postnatal DRG in vivo were investigated. It is noteworthy that about 5% of ganglion cells were still bipolar in the 1-day postnatal rat. When dissociated DRG cells were seen in long-term culture, completely unipolarized DRG cells were revealed by silver-impregnation. But when fibroblast growth was suppressed in long-term culture, no pseudounipolar cells were observed. Dorsal root ganglia on day 14 of gestation were organ cultured. The incidence of pseudounipolar cell appearance was estimated time-sequentially by silver-impregnations (Table 2). Unipolar cells increased to about 80% at the 22nd day of culture. Cultured DRG cells were examined transmission electron microscopy and the morphological change from bipolar cells to pseudounipolar cells was investigated. No evidence was obtained which showed that the processes of bipolar cells fuse to make a stem process. In conclusion, observations of developing DRG cells by light and electron microscopy revealed that the stem process of the pseudounipolar DRG cells is derived from the elongated cytoplasm of the cell body and is not derived from the fusion of the opposite two processes.

Animals↗
PubMed1987

Synergistic inhibitory effects of dipyridamole and vincristine on the growth of human leukaemia and lymphoma cell lines.

The effects of combinations of dipyridamole, an effective blocker of the salvage pathway of DNA synthesis, and 8 types of anti-cancer drugs on the growth of human T, B and myeloid leukaemia/lymphoma cell lines in vitro were examined. In combinations, dipyridamole and vincristine (VCR), and dipyridamole and vindesine had synergistic inhibitory effects. Dipyridamole reduced the efflux of VCR from cells and enhanced their VCR accumulation in a dose-dependent manner at concentrations of up to 10 microM in the lymphoid cell lines, MOLT-3 and BL-TH, and of up to at least 20 microM in the myeloid cell line, ML-1. Dipyridamole also enhanced the accumulation of VCR in PHA-stimulated and un-stimulated lymphocytes of normal donors, but efflux of VCR was more rapid from normal lymphocytes than from cultured cell lines. It is proposed that combination therapy with dipyridamole plus VCR should be effective in the treatment of leukaemia and lymphoma.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

Various expressions of modified myoepithelial cells in salivary pleomorphic adenoma. Immunohistochemical studies.

Variant expressions of modified myoepithelial cells in salivary pleomorphic adenomas are described as determined by immunohistochemical techniques which visualized the distributions of S-100 protein, intermediate-sized filament proteins (keratin, vimentin, and desmin), and contractile proteins (myosin and actin), as well as lysozyme and lactoferrin. Immunohistochemical staining patterns of S-100 protein were basically used to classify modified myoepithelial cells, along with histologic criteria. Histochemical modifications of myoepithelial cells in pleomorphic adenoma of salivary glands could be divided into a) reactive, b) transformed, and c) neoplastic myoepithelial cells. Reactive myoepithelial cells were stromal-like cells which displayed an intense S-100 protein reaction. Transformed myoepithelial cells were negative or slightly positive for S-100 protein; they were located in the outer zone of tubular or duct-like structures and were spindle-shaped. The inner round cells of tubular and ductal structures, which could be ductal origin, gave intense keratin staining, as well as marked reactions for lysozyme and lactoferrin. Neoplastic myoepithelial cells were plasmatoid or fibrous types of cells and contained abundant S-100 protein and vimentin. These cells were termed "myoepithelioma" as in classical diagnosis.

Actins↗