PubMed Health⌕ Search

Biomedical subjects

T Shintani

Publications and source records attributed to T Shintani.

At least 73 records · Page 4Linked to original sources
PubMed1991

The effect of intracerebroventricular administration of somatostatin on prolactin and TSH release in rats.

We investigated the effect of intracerebroventricular (icv) administration of somatostatin (SRIF) on prolactin (PRL) and thyroid-stimulating hormone (TSH) release in freely moving rats chronically cannulated with an atrial catheter. The plasma PRL levels were significantly elevated following the icv administration of 0.5 microgram SRIF. No further increase in PRL following the icv administration of SRIF were found in the rats in the course of repeated intravenous of injection of 5.0 mg/kg sulpiride, a specific D2 receptor antagonist. On the other hand, the injection of 5.0 micrograms SRIF resulted in no significant change in the plasma TSH levels. These results suggest that the effect of SRIF on PRL release was exerted through brain D2 receptors.

Animals↗
PubMed1990

[Effects of uvulopalatopharyngoplasty in adult patients with obstructive sleep apnea syndrome].

We performed uvulopalatopharyngoplasty (UPPP) in 51 adult patients with obstructive sleep apnea syndrome (OSAS). After UPPP, there were statistically significant improvements in apnea-hypopnea index (AHI), the lowest value of oxygen saturation during sleep and total time of apnic episodes. 28 patients (54.9%) were good responders who represented more than 50% improvements in their AHI. Poor responders tended to be more severe and older than good responders. After cephalometric analysis, poor responders were revealed to show significantly poor mandibular prognatism and also lower positioned hyoid bone than good responders.

Adult↗
PubMed1990

[Dilator naris activity in relation to obstructive sleep apnea].

We evaluated the activity of dilator nasalis+ EMG (Electro-Myo-Graphy) and submental EMG during sleep in three patients with obstructive sleep apnea syndrome. Both EMG activities involved periodic changes which proved to be correlated with each other (r = 0.85). Apnea and hypopnea episodes always occurred at the nadir of these periodic changes. The activity of dilator nasalis+ EMG, as well as submental EMG, at the nadir of these periodic changes was significantly lower during REM (Rapid Eye Movement) sleep than non-REM sleep and also significantly lower during severe airway obstruction accompanied by paradoxical movements of thorax and abdomen. The correlation of this EMG activity with SaO2 or the duration of obstructive apnea was more remarkable in submental EMG than dilator nasalis+ EMG in the observed patients.

Adult↗
PubMed1989

Differentiation of intestinal epithelial cell line (IEC-18) by an acid extract of rat small intestine.

A factor which may induce differentiation of intestinal epithelial cell lines in vitro was found in an acid extract of adult rat small intestine. The addition of a partially purified acetic acid extract of rat small intestine to IEC-18 cell culture dishes increased sucrase activity within 48 h. Thymidine incorporation markedly decreased within 24 h. Significant development of microvilli-like structures was observed on the acid extract-treated IEC-18 cells, compared with controls. This activity of rat acid extract was heat-stable and the apparent molecular weight of the factor was 400-800. These findings suggested that the factor may be related to the epithelial differentiation of rat small intestinal crypt cells.

Animals↗
PubMed1987

Metabolism of prostaglandin D2 in isolated rat lung: the stereospecific formation of 9 alpha,11 beta-prostaglandin F2 from prostaglandin D2.

The metabolic transformation of exogenous prostaglandin D2 was investigated in isolated perfused rat lung. Dose-dependent formation (2-150 ng) of 9 alpha,11 beta-prostaglandin F2, corresponding to about 0.1% of the perfused dose of prostaglandin D2, was observed by specific radioimmunoassay both in the perfusate and in lung tissue after a 5-min perfusion. To investigate the reason for this low conversion ratio, we analyzed the metabolites of tritium-labeled 9 alpha,11 beta-prostaglandin F2 and prostaglandin D2 by boric acid-impregnated TLC and HPLC. By 5 min after the start of perfusion, 9 alpha,11 beta-prostaglandin F2 disappeared completely from the perfusate and the major product formed remained unchanged during the remainder of the 30-min perfusion. The major product was separated by TLC and identified as 13,14-dihydro-15-keto-9 alpha,11 beta-prostaglandin F2 by GC/MS. In contrast, pulmonary breakdown of prostaglandin D2 was slow and two major metabolites in the perfusate increased with time, each representing 56% and 11% of the total radioactivity at the end of the perfusion. The major product (56%) was identified as 13,14-dihydro-15-ketoprostaglandin D2 and the minor one (11%) was tentatively identified as 13,14-dihydro-15-keto-9 alpha,11 beta-prostaglandin F2 based on the results from radioimmunoassays, TLC, HPLC, and the time course of pulmonary breakdown. These results demonstrate that the metabolism of prostaglandin D2 in rat lung involves at least two pathways, one by 15-hydroxyprostaglandin dehydrogenase and the other by 11-ketoreductase, and that the 9 alpha,11 beta-prostaglandin F2 formed is rapidly metabolized to 13,14-dihydro-15-keto-9 alpha,11 beta-prostaglandin F2.

Animals↗
PubMed1983

Primary carcinoid tumor of the larynx and review of the literature.

Primary carcinoid tumor of the larynx is very rare. This is the fifth case reported in the literature and the first autopsy case of laryngeal carcinoid. The early manifestation of the present case was multiple metastasis to the skin. At autopsy there was a laryngeal tumor associated with widespread visceral and cutaneous metastases. The tumor proved to be carcinoid tumor by histological, histochemical and electron microscopic findings.

Aged↗
PubMed1980

[Experimental studies on prevention of adhesion of tissue surrounding dura mater and morphological changes in the spinal cord following laminectomy (author's transl)].

The experimental animals were divided into six groups for the following purposes. First, dogs in Group 1 were subjected to laminectomy only to determine the development mechanism of scar tissue surrounding the dura mater and nerve root and to ascertain its effect upon the spinal cord. Next, dogs in Groups 2 to 6 were also subjected to laminectomy, but at the same time various types of interposing membranes were inserted experimentally to prevent adhesion of scar tissue and dura mater and nerve root. In Group 2, Gelfilm was inserted; Group 3, a fibrin membrane; Group 4, Silastic Sheet; Group 5, auto fascia and Group 6, auto fatty tissue. The results are based on findings obtained from 67 mature dogs. The tissue defect created after laminectomy became filled with scar tissue derived from the excised ends of the arch and the dorsal muscles, also representing the cause for adhesion with the dorsal dura mater. Fibrotic tissue extending from the dorsal aspect of the dura mater to the nerve root via the lateral wall joined at the dorsal aspect of the nerve root with the fibrotic tissue extending directly towards the nerve root from the excised ends of the arch. Adhesion between scar tissue and the dura mater of the lateral wall and the nerve root was observed 4 months after surgery. The use of interposed membranes after laminectomy suppresses fibrotic proliferation between the excised ends of the arch and nerve root and also inhibited fibrotic growth along the dorsal aspect to the lateral wall of the dura mater, and the results showed that at 4 months after surgery, inhibition in formation of scar tissue was noted in the dorsal aspect of the nerve root. A slight degree of flattening of the spinal cord was found in the laminectomy only group, but no such flattening could be seen in the fatty tissue transplanted group.

Animals↗
PubMed1979

The release and metabolism of pancreatic hormones after major hepatectomy in the dog.

Major hepatectomy in the dog induced a 50% decrease in peripheral serum glucose, a 11-fold increase in portal plasma glucagon and a 36-fold increase in the portal glucagon/insulin ratio 3 hr after operation. Peripheral serum glucose levels were inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.50, p less than 0.01) and that of the portal glucagon/insulin ratio (r = -0.85, p less than 0.01) for 1-6 hr after operation. The ratio of peripheral to portal plasma glucagon was also inversely correlated to the logarithmic value of portal plasma glucagon (r = -0.59, p less than 0.01). In case of glucose infusion, plasma glucagon levels were not elevated after major hepatectomy. The data suggest that glucose deficiency after major hepatectomy in the dog may cause hyperglucagonemia with an enhanced glucagon requirement.

Animals↗
PubMed1978

Lymphocyte cytotoxicity to cultured rat liver cells in patients with chronic liver diseases.

Microcytotoxicity assay revealed that peripheral bloof lymphocytes from patients with chronic active hepatitis were cytotoxic against cultured rat liver cells established by Coon in 1968. Non E-rosette forming cells were cytotoxic in 26 of 28 patients (93%) with chronic active hepatitis, whereas E-rosette forming cells were cytotoxic in only 1 of them. Either an addition of 10 microgram/well of aggregated IgG to non E-rosette forming cell culture or a preincubation of non E-rosette forming cells with 100 microgram/ml of aggregated IgG significantly reduced the cytotoxicity from 62.9 +/- 12.8% to 32.8 +/- 11.6% or to 25.6 +/- 11.3% (p less than 0.001). An addition of antihuman IgG/Fc also reduced the cytotoxicity to 37.4 +/- 17.2%. Significant cytotoxicity of positively selected EA-rosette forming cells was observed in 4 of 10 patients with chronic active hepatitis and that of positively selected EAC-rosette forming cells was demonstrated in 3, whereas in any of these patients neither non EA-rosette forming cells nor non EAC-rosette forming cells were cytotoxic. Cultured liver cells used in this study were seen to possess insoluble liver specific antigen on their surface membranes, but not soluble liver specific lipoprotein of Meyer zum Büschenfelde, by using an indirect immunofluorescence technique. These results suggested that effector cells are Fc-receptor-bearing cells and that the mechanism of the reaction may be mediated in an antibody-dependent cell-mediated reaction directed against insoluble liver specific membrane antigen(s) rather than soluble one.

Animals↗