Biomedical subjects

T Takatori

Publications and source records attributed to T Takatori.

168 records · Page 10Linked to original sources

PubMedDate not supplied

Separation and identification of 9-chloro-10-methoxy (9-methoxy-10-chloro)hexadecanoic and octadecanoic acids in adipocere.

Two homologs of 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids were found in adipocere from the human neonate, and identified as 9-chloro-10-methoxy(9-methoxy-10-chloro)hexadecanoic acid and 9-chloro-10-methoxy(9-methoxy-10-chloro)-octadecanoic acid. The chemical structures of these compounds were confirmed by thin-layer chromatography, gas-liquid chromatography, gas chromatography--mass spectrometry, nuclear magnetic resonance spectrometry and elemental analysis. The adipocere contained approximately 7.2% 9-chloro-10-methoxy(9-methoxy-10-chloro) fatty acids in the total fatty acids.

Chromatography, Gas↗

PubMedDate not supplied

Isolation of intact plankton from drowning lung tissue by centrifugation ina colloidal silica gradient.

Several species of intact phyto- and zooplankton from the homogenate of rat "drowning lung" were separated by centrifugation in a colloidal silica gradient. The plankton, except diatoms, were isolated from a small amount of human "drowning lung" by this procedure. This new method was found to be more useful in detecting plankton in tissues than chemical digestion with strong acid.

Animals↗

PubMedDate not supplied

Isolation of spermatozoa in vaginal contents by centrifugation in a colloidal silica gradient.

Human spermatozoa were separated from vaginal contents with epithelial cells by density gradient centrifugation in colloidal silica coated with polyvinylpyrrolidone. In this procedure spermatozoa were collected at a density range of 1.130 - 1.142 g/ml by centrifugation at 35 000 g for 1 h.

Centrifugation, Density Gradient↗

PubMedDate not supplied

The determination of choline in human semen by the enzymic method.

Studies are reported on the specific enzymic microdetermination of free choline in human semen. The concentration of choline in normal semen was found to be 0.9 - 1.4 mg/ml. The minimum detectable concentration of choline using the method described was 1.5 micrograms. Normal levels of choline in vaginal fluid, saliva, serum and urine could not be detected by this procedure.

Alcohol Oxidoreductases↗

PubMedDate not supplied

Identification of 10-hydroxy-12-octadecenoic acid in adipocere.

Studies are reported on separation and identification of 10-hydroxy-12-octadecenoic acid in human adipocere. The chemical structure was determined by thin-layer chromatography (TLC), gas-liquid chromatography (GLC) and gas chromatography-mass spectrometry (GC-MS).

Chromatography, Gas↗

PubMedDate not supplied

Microbial production of hydroxy and oxo fatty acids by several microorganisms as a model of adipocere formation.

Some varieties of aerobic or anaerobic microorganisms from the human stool and adipocere were separated and identified. These separated microorganisms together with other authentic ones produced 10-hydroxystearic acid from oleic acid. Some bacteria could convert oleic acid to 10-oxostearic acid as well as 10-hydroxystearic acid. These findings indicate that the microbial enzyme(s) catalyzes the hydration of oleic acid and probably the oxidation of this hydrated product. Aerobic bacteria as well as anaerobic microorganisms were found to be involved in the formation of adipocere.

Bacillus subtilis↗