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Biomedical subjects

T Takatori

Publications and source records attributed to T Takatori.

At least 163 records · Page 9Linked to original sources
PubMed1976

Effects of dietary saturated and trans fatty acids on cholesteryl ester synthesis and hydrolysis in the testes of rats.

Studies were made of the enzymic synthesis and hydrolysis of cholesteryl esters in rat testes. Weanling rats were fed for 14 weeks diets containing 5% by wt of hydrogenated coconut oil (HCO), a concentrate of ethyl elaidate and linolelaidate (TRANS), devoid of essential fatty acids (EFA), or safflower oil (SAFF). Cholesterol esterifying activity was localized in the soluble fraction, and cholesteryl ester hydrolase activity was distributed in both particulate and soluble fractions obtained from tissue homogenates. The optimum pH was 6.0 for esterification and 6.9-7.0 for hydrolysis. Neither esterifying nor hydrolytic activity was affected by freezing and thawing, but both reactions were inhibited by heat or sonication. The animals of both the HCO and TRANS groups had developed an EFA deficiency before they were sacrificed. The EFA deficiency produced upon feeding the HCO diet had no apparent effect on the synthesis and hydrolysis of cholesteryl esters in rat testes. The TRANS diet influenced the development of the testes as judged by their size, and cholesterol esterifying and cholesteryl ester hydrolyzing activities were suppressed in the testes of the animals of this group. A major difference in the effects of the HCO and TRANS diets on the lipids of the tests was the relatively minor amount of eicosatrienoic acid (20:3) and the elevated level of docosapentaenoic acid (22:5) in the cholesteryl esters of the testicular lipids of the TRANS group.

Animals↗
PubMed1976

Effects of dietary saturated and trans fatty acids on tissue lipid composition and serum LCAT activity in the rat.

Groups of rats were fed from weaning with diets containing 5% by wt of hydrogenated coconut oil (HCO), safflower oil, or a concentrate of ethyl elaidate and linolelaidate (TRANS) as the sole source of dietary fat. Fatty acid composition of the lipid classes from serum, liver, heart, and kidney was determined, and the serum lecithin: cholesterol acyl transferase (LCAT) activities were assayed for each animal. Serum LCAT activity was increased by both the HCO and TRANS diets in the early stages of the development of an essential fatty acid (EFA) deficiency but was suppressed in the animals of the TRANS group as they became older. The HCO and TRANS groups exhibited changes in tissue lipid fatty acid composition, as well as reduced growth, characteristic of an EFA deficiency. Coversion of oleic acid to eicosatrienoic acid was impaired in the animals fed the TRANS diet, greatly increasing the octadecenoic acid content of the tissue lipids at the expense of eicosatrienoic acid. The TRANS diet also suppressed incorporation of eicosatrienoic acid into cholesteryl esters of tissue and serum, indicating that, when fed as the sole source of unsaturated fat, trans fatty acids influenced the metabolism of unsaturated fatty acids and cholesterol.

Acyltransferases↗
PubMed1976

[A statistical study of autopsy cases in the department of legal medicine, Hokkaido University School of Medicine, during the last 10 years (author's transl)].

The mediocolegal autopsies performed in the Department of Legal Medicine, Hokkaido University School of Medicine, during the 10-year period, 1965-1974 were 617 cases, 87 of them in newborns, 530 in infants and adults. Of cases autopsied as homicidal or suspicious deaths in infants and adults, 88.5% were recognized to be due to external causes, 9.1% to disease conditions and 2.5% undetermined. Of neonatal deaths, 10.3% were stillbirth, 78.2% live birth and 11.5% undetermined. Among the deaths due to external causes in infants and adults, 43.3% were homicidal, 7.2% suicidal, 32.6% accidental and 16.6% undetermined. Of external factors causing death, 42.9% were force, 36.8% asphyxiaton, 8.0% chemical substances and 8.0% heat. The causes of homicidal deaths inflicted with cutting or piercing instruments were circulatory disturbance, asphyxia and infection. In deaths inflicted with blunt instruments, they were mostly injuries of central nervous system and circulatory disturbance in the minority. All categories of causes of death were recognized in accidental deaths. All neonatal deaths were due to external causes. Their causes of death were mostly asphyxia and blunt force injuries of head.

Adolescent↗
PubMed1976

Separation and estimation of short-chain fatty acids and organic acids by thermodetective liquid chromatography.

Short-chain fatty acids and organic acids in aqueous solution were separated quantitatively by using a liquid chromatography. JLC-2A, which posseses a thermodetector with ion-exchange resin. A peak height of thermogram showing a thermal difference caused by adsorption and desorption of substance was proportional to the concentration. Each sample was detectable at least between approximately 10(-5) and 2 X 10(-3) moles. The "inter-day" coefficient of variation obtained from two peak heights of acetic and n-butyric acids was 1.9 and 2.3%, respectively.

Acetates↗
PubMed1976

[Studies on biosynthesis of short-chain fatty acids (author's transl)].

Studies were reported here on biosynthesis of short-chain fatty acids in lactating rabbit mammary glands. The maximal incorporation from [1-14C] acetate into total fatty acids were observed in microsomes and supernatant fractions of mammary glands but the synthetic rate either in the microsomes alone or in the supernatant alone was rather low. However approximately 80% of the maximal rate was restored in the supernatant for n-butyric acid synthesis. The incorporation from [1-14C] acetate into-n-butyric acid was markedly stimulated in the presence of NADH, compared to NADPH while total fatty acids synthesis was more dependent on NADPH. Long-chain fatty acids synthesis from [1-14C] acetate was decreased markedly by the addition of avidin although n-butyric acid formation was restored to 80%. Then by the addition of malonyl CoA or biotin the avidin system, middle- and long-chain fatty acids were recovered again. [1-14C] propionate was incorporated into even-numbered chain fatty acids as well as odd chain fatty acids. The synthesis of total fatty acids from [1-14C] propionate was more dependent on NADPH-generating system than either NADH or NADPH. [1-14C] bicarbonate was also incorporated slightly into fatty acids, such as decanoic or dodecanoic acids. In the reduction from either acetoacetyl CoA or crotonyl CoA, the tritium of NADP3H was stereospecifically incorporated into the beta-position of n-butyric acid. The reaction of acetoacetyl CoA reduction was much more dependent on NADH while crotonyl CoA was more reduced with NADPH. There was no difference between the dependencies on NADH and NADPH in the reduction of 2-hexenyl CoA.

Acetates↗
PubMed1976

Toxic effects of hydroperoxide injections on rat lung. A light microscopical and ultrastructural study.

Intravenous administration of methyl linoleate hydroperoxide (MLH) uniformly resulted in extensive damage to septal wall components of the rat lung; the severity of these changes paralleled the administered dosage. The target cell of injury appeared to be the capillary endothelial cell. Destructive changes within the plasma membrane suggested that the major toxic effect of MLH was directed against membranous structures. Ultrastructural analysis showed that these effects were more severe in the vitamin E-deficient animals. Secondary changes were characterized by interstitial and alveolar edema, and degenerative changes within alveolar pneumocytes. Reparative proliferation of type 2 pneumocytes first became evident in animals that survived two days. These observations provide an insight into the susceptibility and sequential damage to pulmonary tissue by hydroperoxides and serve as a guide for investigations of the toxic effects of other oxidants on lung.

Animals↗
PubMed1975

Studies of lipid class and fatty acid profiles of rat mammary tumors induced by 7, 12-dimethylbenz(a)anthracene.

The lipid class and fatty acid composition profiles of mammary glands of female rats fed a nutritionally adequate diet are compared to those of tumors induced in the mammary glands by intravenous injection of dimethylbenz(a)anthracene of animals fed the same diet. Ca. 95% of the lipids of the mammary glands of the control group of animals consisted of triglycerides; glycolipids and phospholipids were present in only minor amounts. In contrast, the lipids of the mammary tumors contained much lower amounts of neutral lipids and higher concentrations of phospholipids. The glycolipid fraction was a minor component of both tissues but differed greatly in composition. The composition of the phospholipid and neutral lipid fractions, particulary the latter, of the mammary tumors also differed from that of the mammary glands of the control animals. The neutral lipids of the tumor tissues contained elevated levels of free fatty acids and cholesterol and much lower concentrations of triglyceride compared to the mammary gland lipids. Differences also were observed in the fatty acid composition of tumor and mammary gland lipid. The greatest differences occurred in the concentrations of polyunsaturated fatty acids which were generally much higher in the tumor lipids.

Animals↗
PubMed1975

Studies on stereospecific reduction of acetoacetyl CoA and crotonyl CoA in lactating rabbit mammary glands.

Stereospecificity as well as the dependency on reduced pyridine nucleotides of the enzymatic reduction of acetoacetyl CoA and crotonyl CoA in lactating rabbit mammary glands are reported. In the reduction of both acetoacetyl CoA and crotonyl CoA as substrates the tritium from NADP3H was stereospecifically incorporated into the beta-position of n-butyric acid. The reaction of acetoacetyl CoA reduction was much more dependent on NADH while the reduction of crotonyl CoA was rather more dependent on NADPH. There was no difference between the dependencies on NADH and NADPH in the reduction of 2-hexenyl CoA.

Acetyl Coenzyme A↗
PubMed1975

Effect of ozone exposure on lecithin : cholesterol acyltransferase activity in rat serum.

Studies are reported here of relationships of ozone exposure and lecithin : cholesterol acyltransferase (LCAT) activity in rat serum. Exposure to low concentrations of ozone (1.1+/-0.27 ppm) for 24 hrs, significantly elevated LCAT activity, while the concentration of free cholesterol in rat serum was little changed during the exposure. In contrast, LCAT activity was decreased by exposure to high concentrations of ozone (2.0-2.5 ppm) for 24 hrs.

Acyltransferases↗
PubMedDate not supplied

The mechanism of adipocere formation 1. Identification and chemical properties of hydroxy fatty acids in adipocere.

The hydroxy fatty acid purified from the adipocere, m.p. 78.5 degrees -79.0 degrees C, was optically inactive and the adipocere contained about 3 to 20% hydroxy fatty acid of the total fatty acids. The melting point of the hydroxy fatty acid was nearly identical with that of the 10-D, L-hydroxyoctadecanoic acid synthesized organically, suggesting that the hydroxy fatty acid in the adipocere appears to be converted non-enzymatically. In the adipocere two hydroxy fatty acid components were detected by gas-liquid chromatography (GLC). The major and minor components were identified as 10-hydroxyoctadecanoic and 10-hydroxyhexadecanoic acids, respectively, by gas chromatography-mass spectrometry (GC-MS). These hydroxy fatty acids found in the adipocere appear to play an important role on the formation of adipocere.

Adipose Tissue↗
PubMedDate not supplied

The mechanism of adipocere formation. II. Separation and identification of oxo fatty acids in adipocere.

Two kinds of oxo fatty acids were found to be present in the adipocere. One of them was identified as 10-oxohexadecanoic acid and the other was as 10-oxo-octadecanoic acid. The chemical identity of these compounds was confirmed by thin-layer chromatography (TLC), gas-liquid chromatography (GLC) and gas chromatography-mass spectrometry (GC-MS). The adipocere contained about 1.3 to 1.6% oxo fatty acid of total fatty acids.

Chromatography, Gas↗