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Biomedical subjects

T Takatori

Publications and source records attributed to T Takatori.

At least 127 records · Page 7Linked to original sources
PubMed1985

Methane asphyxia. Coal mine accident investigation of distribution of gas.

Death from asphyxia due to substitution of air by methane gas may occur in coal mine by gas outburst. In such a case, it is required to determine methane gas contents from cadaveric blood and tissues for diagnosing cause of death and estimating conditions of the accident. The methane concentration in blood and tissue samples of 22 male victims by a gas outburst accident was measured by gas chromatography. The level of methane in the cardiac blood was in the range of 6.8-26.8 microliters/g. As a model of gas outburst in coal mine, rats were exposed experimentally to various concentrations of methane. Their course of death and methane distribution in the bodies were observed. From these findings, diagnostic criteria for asphyxia from substitution of air by methane are also discussed.

Accidents, Occupational↗
PubMed1984

A medicolegal study on enzymic fluorometry of choline in human semen.

Studies have been conducted on an enzymic fluorometric method based on an initial rate of reaction for the determination of choline. The reaction system consists of choline oxidase coupled to peroxidase and homovanillic acid. Concentrations of choline as low as 0.1 nmol could be detected by this procedure. The concentration of free choline in normal semen was 18.7 to 29.5 mumol/mL. Free choline in other body fluids was negligible. The choline concentrations in seminal stains maintained at room temperature were not changed during a 30-day period. Those concentrations in seminal fluids kept at room temperature were detected until at least the fifth day.

Alcohol Oxidoreductases↗
PubMed1980

Further production and characterization of antibodies reactive with pyrazolone derivatives.

A sensitive radioimmunoassay for pyrazolone derivatives has been developed. Anti-antipyrine antisera were produced in rabbits by repeated immunization with 4-succinamidoantipyrine coupled to bovine serum albumin. Less than 1 ng of antipyrine could be detected by this procedure. Various substituents on the carbon-4 position of the pyrazolone ring decreased the affinity for the antibody. The concentrations in ng of various pyrazolone derivatives required to inhibit [3H]antipyrine binding by 50% were: antipyrine, 6.8; aminopropylon, 8.5; sulpyrine, 35.5; isopropylantipyrine, 1320; and aminopyrine, 2820. The antibody showed no cross-reactivity with any other antipyretics such as pyrazolidine or aniline derivatives. The determination of antipyrine and sulpyrine concentrations in rat serum after i.p administration was also carried out.

Aminopyrine↗
PubMed1980

Histopathologic study of adverse effects of linoleic acid on rat lung.

Linoleic acid administered intravenously was highly toxic to the rat lung. The severity of the toxicity paralleled the dosage administered and was characterized by generalized, irreversible damage to alveolar wall structures. Although evidence of tissue damage was not apparent or minimal by light microscopy, ultrastructural studies disclosed extensive degeneration and necrosis of septal wall components with 15 minutes following injection of low dosages of linoleic acid. Degeneration of endothelial cells was most severe suggesting that these cells were the target of injury resulting in impaired vascular integrity, interstitial and alveolar edema, and degeneration of type 1 pneumocytes. Fragmentation and stripping of endothelium from capillary basement membranes was uniformly present and was in some instances associated with platelet aggregation indicative of early thrombosis. These studies suggest that this toxic effect on septal capillaries leading to increased permeability and alveolar edema may be a contributing factor to death occurring from free fatty acid toxicity in the human.

Animals↗
PubMed1980

Gas chromatographic determination of kerosene components from cadaveric hypopharyngeal contents.

For the purpose of suicide, a man sometimes pours kerosene or gasoline on himself and fires it. In this case in order to clarity the fact that a man killed himself by using kerosene or gasoline, the chemical characteristic of the fuel should be examined from some parts of the burned body. Recently a female burned body was performed autopsy to examine her cause of death and a means of her death as well as to aid in her identification. In this paper the gas chromatographic determination of some saturated hydrocarbons from her hypopharyngeal contents smelling of kerosene were attempted to estimate the means of her death from burns.

Cadaver↗
PubMed1979

Radioimmunoassay for pyrazolone derivatives.

The determination of pyrazolone derivatives by radioimmunoassay has been reported. Anti-antipyrine antisera were obtained by repeated immunization of rabbits with 4-azoantipyrine-conjugated bovine serum albumin. The level at least as low as 1.0 ng of antipyrine could be detected by this procedure. Various substituents on the carbon 4 position of the pyrazolone ring as well as the lack of methyl group on the nitrogen 2 position decreased the affinity for the antibody, and the antibody showed no cross-reaction with any pyrazolidine derivatives.

Ampyrone↗
PubMed1979

Determination of phenobarbital by radioimmunoassay.

A radioimmunoassay for phenobarbital has been studied. Antiphenobarbital antisera were obtained by repeated immunization of rabbits with p-succinamidophenobarbital conjugated to bovine serum albumin. Less than 0.2 pmol of phenobarbital could be measured by this procedure. The specificity of the antibodies was directed to substituents on the nitrogen atoms of the barbituric ring as well as to substituents at the carbon 5-position of the ring.

Antibody Formation↗