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T Ueda

Publications and source records attributed to T Ueda.

At least 1,477 records · Page 82Linked to original sources
PubMed1981

Attachment of the synapse-specific phosphoprotein protein I to the synaptic membrane: a possible role of the collagenase-sensitive region of protein I.

The purified synapse-specific phosphoprotein Protein I was previously shown to be degraded by a bacterial collagenase, through a series of intermediates, to a collagenase-resistant fragment of molecular weight about 48,000 containing a phosphorylated serine residue. In this study, a purified synaptic membrane fraction containing Protein I was treated with Cl. histolyticum collagenase; membrane-bound and membrane-free proteins were then phosphorylated using [gamma-32P]ATP and analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography. It was observed that Protein I bound to the synaptic membrane was susceptible to the collagenase and degraded to fragments of molecular weights about 68,000, 62,000, and 48,000; the 68,000 fragment remained bound to the membrane whereas the 62,000 and 48,000 fragments were dissociated from the membrane. These observations suggest that the peptide moiety of mol. wt. 6000, present in the 68,000 fragment but absent from the 62,000 fragment, may play a crucial role in anchoring Protein I to the synaptic membrane.

Animals↗
PubMed1981

Echocardiographic assessment of Qp/Qs in children with atrial septal defect or partial anomalous pulmonary venous connection.

In 24 children with atrial septal defect of the secundum type and/or partial anomalous pulmonary venous connection, the pulmonary to systemic blood flow ratio (Qp/Qs) was estimated by echocardiography. Using M-mode echocardiography, right ventricular diameter (RVD), left ventricular diameter (LVD), total cardiac diameter (TCD) and aortic root (AOR) were measured, and the following 5 indices were obtained: RVD/LVD, RVD/TCD, RVD/AOR, RVD/BSA (body surface area) and TCD/BSA. The single linear correlation coefficients between these 5 indices and Qp/Qs, obtained by the Fick method during cardiac catheterization, were 0.88, 0.80, 0.74, 0.75 and 0.55, respectively. Thus, RVD/LVD ratio was the most sensitive echocardiographic index to assess Qp/Qs in these children. Clinically, however, the multiple linear regression equation derived from 3 indices (RVD/BSA, RVD/LVD and TCD/BSA) might be more useful (r = 0.89). Although the abnormal motion of the interventricular septum was analyzed, the quantification of this motion as an index of Qp/Qs was difficult.

Child↗
PubMed1981

Idiopathic acute myocarditis with complete atrioventricular block in a baby. Clinicopathological study of the atrioventricular conduction system.

A 17-month-old Japanese girl with an idiopathic acute myocarditis had symptoms of vomiting, slight fever, and liver enlargement, but no edema. Clinical diagnosis of acute myocarditis was not made until she had Stokes-Adams syndrome and electrocardiogram revealed complete atrioventricular block on the day of death. At autopsy, idiopathic acute myocarditis was detected diffusely in the right and left ventricles. Vomiting and liver enlargement were due to congestive heart failure. Serial sections of the atrioventricular conduction system revealed diffuse and severe acute inflammatory changes in the right bundle and the left bundle branches, especially in the terminal portions. Acute inflammation was focally noted in the atrioventricular node and the His bundle. The complete atrioventricular block probably followed the severe acute inflammation of the bundle branches. Our case suggest that idiopathic acute myocarditis may be underdiagnosed in babies, as there is no way to determine whether there is dyspnea and palpitation on exertion, and idiopathic fibrosis of conduction system with or without conduction disturbances in children and adults may be sequelae of healed myocarditis in babies.

Acute Disease↗
PubMed1981

Mode of degradation of tRNAs with ozone.

Ozone-treatment of tRNA resulted in the degradation of guanine residues located on the loop regions, such as the anticodon and D-loop region. In addition, it became evident that the guanine residues in the consecutive sequences, such as G-G-G-G and G-G-m1G in tRNAPro, were the most susceptive to the ozone-treatment. The internucleotidic linkage of the treated tRNA was not cleaved but several fragments were obtained by a gel electrophoretic separation after heating at 60% in 1M aniline-acetate buffer (pH 4.5). Major fragments derived from tRNAPro were the 3'-half and the 5'-half molecules produced by the cleavage at the anticodon region.

Anticodon↗
PubMed1981

Effects of room temperature on reproduction, body and organ weights, food and water intake, and hematology in rats.

Two generations of rats were raised at animal room temperature ranging from 12 to 32 degrees C at steps of 2 degrees C. The body weight of rats born in 22 degrees C environment and exposed to each temperature did not significantly differ within the range of 16-28 degrees C. The delivery rate, litter size, and weaning rate decreased at 30 degrees C and 32 degrees C, and only the weaning rate had a tendency to decrease at 12 degrees C. No significant difference was demonstrated within the 14-28 degrees C range for any reproduction parameter observed. The body weight of sucklings did not differ at birth within the 12-32 degrees C range; and at 3 weeks of age, there were no differences within the range of 18-28 degrees C. The body weight gain in both sexes after weaning was generally small when the temperature was below 18 degrees C or above 30 degrees C. There were no significant differences in food intake within the 20-26 degrees C range, in water intake within the 12-26 degrees C range, in hematological and serum biochemical values within the 20-26 degrees C range, and in organ weights within the 18-28 degrees C range. Therefore, the range of 20-26 degrees C (68-78 degrees F) was the optimum temperature range in rat rooms.

Animals↗
PubMed1981

The effect of longitudinal tension on the amplitude of the contraction rhythm of plasmodial strands of Physarum polycephalum.

Plasmodial strands of Physarum polycephalum exhibit a characteristic contraction rhythm. The amplitude of the isotonic tension increased linearly with the cube root of relative stress. The period of the isometric tension increased linearly with the amplitude of the oscillation, but stayed constant above a critical value. The above empirical formulae were found applicable for all temperatures employed, 9 to 28 degrees C, but different temperature coefficients were observed below and above 15 to 16 degrees C.

Cytoplasm↗
PubMed1980

Conversion of positive to negative chemotaxis by Ca2+ ionophore treatment in plasmodium of Physarum polycephalum.

The plasmodium of Physarum polycephalum was treated with Ca2+ ionophores A23187 or X-537A, and the effects on the chemotactic and contractile activities were studied by applying a double-chamber method and an optical method. (1) At low Ca2+ concentration, attractants (glucose, L-alanine) KH2PO4) remained as attractants and induced a relaxation in the plasmodial strand. Increase in the external Ca2+ level caused a contraction of plasmodium with the same stimuli, and hence converted the attractants to repellents. (2) This phenomenon was specific to Ca2+; Mg2+, Sr2+ or Ba2+ showed no appreciable effects. (3) Repellents (sucrose, KCl, Quinine) induced a contraction, which was not altered by the Ca2+ ionophore treatment. (4) Efflux of Ca2+, which was measured by chemiluminescence of aequorin, increased on glucose reception in the presence of ionophore.

Anti-Bacterial Agents↗
PubMed1980

Comparative studies on absorption patterns of the drug after intrarectal administration of 5-FU emulsion and 5-FU solution.

Intrarectal administration of 5-fluorouracil (5-FU) emulsion was compared experimentally with that of 5-FU solution as to the degree of absorption and tissue distribution. For the 5-FU levels in the rectal wall, the levels in the emulsion group remained at high levels during the 6 hour period after administration while the levels in the solution group decreased rapidly. For the 5-FU levels in the lymph nodes, those in the emulsion group were significantly higher (more than four times) and remained at relatively high levels for a longer period than those in the solution group. In the thoracic lymph, 5-FU levels in the emulsion group remained more than two times higher throughout the experimental period. The results suggest tha preoperative intrarectal administration of 5-FU emulsion may be effective as an adjunct to surgery for rectal cancer with lymph node metastasis, because of the long duration of high concentration resulting in the rectal lymphatics and rectal wall.

Animals↗
PubMed1980

Membrane biophysics of chemoreception and taxis in the plasmodium of Physarum polycephalum.

The threshold phenomena observed in chemoreception and taxis of the plasmodium of Physarum polycephalum were analyzed on the basis of physical chemistry. Various physicochemical concepts and rules, e.g. the Schulze-Hardy rule, the lyotropic number and the hydrophobic interactions, were shown to be applicable reasonably well to the physiological functions in Physarum. It was stressed that the structural change of the surface membrane induced by reception of chemical stimuli plays a decisive role in recognition and sensitivity to the external stimuli as well as the appearance of tactic movement in the amoeboid motility of Physarum.

Journal Article↗