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Biomedical subjects

T Ueda

Publications and source records attributed to T Ueda.

At least 1,459 records · Page 81Linked to original sources
PubMed1982

[Administration of vindesine sulfate for the treatment of malignant hematological tumors].

Forty-six cases with hematological malignancies were treated with vindesine sulfate (VDS); a new semisynthetic vinca alkaloid. Six cases with ALL, 5 cases CML in blastic crisis, 3 Hodgkin's disease (HD) and 4 non-Hodgkin's lymphoma (NHL) were treated with VDS alone. Five out of 6 cases ALL, 2 out of 5 CML in blastic crisis were induced into partial remission with VDS alone. All of 3 HD, and 4 NHL were induced in complete remission (CR) or partial remission (PR). Out of 5 cases AML in CR who received VDS as the maintenance therapy in combination with cyclophosphamide, 6-MP and prednisone, one case relapsed during the treatment, but other four cases maintained CR for 4 to 24 months. One case of APL in relapse, which was treated with VDS and 6-MP, reinduced into CR after one month. Out of 16 cases with malignant lymphoma treated by combination chemotherapy including VDS, eleven cases entered in CR or PR. Out of four cases in which the disease became refractory to vincristine (VCR) or vinblastine (VLB) clinically, two achieved PR. VDS was administered intravenously with 3 mg/body/week. When undesirable effect such as leucocytopenia was observed, the dose was reduced to 2-2.5 mg/body/week or 3 mg/body/2 weeks or month. Neurotoxicity (i.e. Paresthesia 21.7%), alopecia (21.7%), leucocytopenia (19.6%), constipation (10.9%) and fever (6.5%) were main side effects of VDS. The neurotoxicity of VDS, however, seemed far less intensive than VCR.

Acute Disease↗
PubMed1982

[Vasculo-Behçet's disease with superior sagittal sinus thrombosis--case report (author's transl)].

The patient is a 30-year-old man who has suffered from recurrent attacks of tonsilitis, oral aphthae and scrotal ulcerations, erythema nodosum and thrombophlebitis. In April, 1980, he gradually developed headache and visual disturbance. On April 14, 1980, he was pointed out remarked bilateral choked disc by an ophthalmologist and then admitted to the Miyazaki Medical College Hospital. On admission to our service, he showed atypical symptoms of Behçet's disease, namely, oral aphthae and scrotal ulcerations, erythema nodosum and bilateral choked disc. Laboratory data demonstrated hyperimmunoglobulinemia, increased clotting factors and decreased fibrinolytic activity. Immunogenetically, HLA BW51 type was demonstrated. The angiograms showed complete obstructions of the superior sagittal sinus and the common trunk of the femoral artery. Histological examination of the skin lesion demonstrated atypical chronic inflammation and thrombophlebitis. A diagnosis of atypical Vasculo-Behçet's disease was made. The response to the steroid therapy was dramatic, though the fibrinolytic drugs, anticoagulants and vasodilators were not effective. Thrombophlebitis is a well recognized complication of Behçet's disease occurring in major vessels, however thrombosis of the dural sinus has rarely reported. This case may be the first one which had superior sagittal sinus thrombosis with Vasculo-Behçet's disease in literature. We discussed the mechanism of the thrombogenesis, the relationship to HLA, the coexistence of Neuro-Behçet's disease and the therapy of Vasculo-Behçet's disease.

Adult↗
PubMed1982

Synthesis of carbon-bridged pyrimidine cyclonucleosides.

6,5'-Cyclo-5'-deoxyuridine 2',3'-cyclic phosphate and its derivatives were prepared as the conformationally fixed substrates of pancreatic ribonuclease. 6,6'-Cyclouridine, 6,3'-methylene-cyclo-2', 3'-dideoxyuridine, and 6,2'-ethylene-cyclo-2'-dexoyuridine were prepared as the new types of C-cyclopyrimidine nucleosides fixed in the anti conformation. The relationship between the sign and magnitude of CD Cotton bands and glycosylic torsion angles is discussed.

Animals↗
PubMed1981

Affinity-purified anti-protein I antibody. Specific inhibitor of phosphorylation of protein I, a synaptic protein.

Protein I is a synaptic protein which serves as an endogenous substrate for cyclic AMP- and calcium-dependent protein kinases. Antibodies raised against purified Protein I have been isolated from rabbit antiserum by affinity chromatography on Protein I-conjugated agarose column. The purified antibodies were identified as immunoglobulin G by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Ouchterlony double immunodiffusion precipitation test. The purified antibodies not only inhibited the phosphorylation of purified Protein I by exogenous cyclic AMP-dependent protein kinase, but also inhibited specifically the phosphorylation of Protein I by endogenous cyclic AMP-dependent protein kinase in a synaptic vesicle fraction, synaptic junctional complex fraction, synaptic membrane fraction, and crude homogenate of cerebrum. The purified anti-Protein I antibodies also inhibited with a similar potency calcium-dependent phosphorylation of Protein I without affecting the phosphorylation of other proteins. The Fab(t) fragment of anti-Protein I immunoglobulin G, which was produced by tryptic digestion, retained the ability to inhibit specifically the phosphorylation of Protein I. This substrate-directed, specific inhibitor of the phosphorylation of Protein I may provide a unique probe for investigating the function of Protein I phosphorylation.

Animals↗
PubMed1981

Synthesis and molecular conformation of 2',3'-O-isopropylidene-5'-deoxy-6(R),5'-cyclo-5,6-dihydrouridine.

The title compound (hereafter abbreviated as 6(R),5'-cyclo-hUrd) is synthesized from 2',3'-O-isopropylidene-5'-deoxy-5'-iodouridine and its molecular structure has been determined by X-ray analysis. 6(R),5'-Cyclo-hUrd crystallizes in space group C2 with Z = 4, and unit-cell dimensions a = 11.220 (2), b = 6.393 (1), c = 18,963 (3) A and beta = 107.98 (1) degrees. The structure was solved by direct interpretation of the three-dimensional Patterson function and refined to a final R index of 0.063. In the crystal the glycosyl torsion angle chi CN is 60.7 degrees (anti conformation) and the dihydrouracil ring adopts a half-chair conformation. The puckering of the ribose ring is an unusual O(1')-exo (P = 267 degrees, tau m = 47 degrees). The coupling constants of the 1H-NMR spectrum measured in C2HCl3 solution indicate that the overall conformation of 6(R),5'-cyclo-hUrd found in crystal is also maintained in solution. The theoretical calculations of coupling constants by the finite perturbation theory (FPT) intermediate neglect of differential overlap, self-consistent field molecular orbital (INDO SCF-MO) method indicate that the deviation of the observed coupling constants of sugar ring protons from those predicted by applying modified Karplus-type formula to the X-ray structure could be due to the strains around the sugar ring carbon atoms attached by protons.

Chemical Phenomena↗
PubMed1981

Left ventricle volume characteristics in children with tricuspid atresia before and after surgery.

Left ventricular volume variables (left ventricular end-diastolic volume, ejection fraction, mass and systolic output) were quantified in (1) 13 patients with tricuspid atresia preoperatively (type Ia, 3 patients; type Ib, 9 patients; type IIb, 1 patient), (2) 4 patients after a shunt procedure (Blalock-Taussig, 3; Glenn, 1), and (3) 1 patient after corrective surgery. Cardiac catheterization and angiography were performed at age 1 month to 5.8 years (mean 1.4 years). All patients had decreased pulmonary blood flow. The results were compared with data in 15 patients with no significantly greater than normal in 12 of the 13 patients. Left ventricular mass was significantly greater than normal, but ejection fraction was significantly less than normal. There was a positive correlation between ejection fraction and systemic oxygen saturation. Left ventricular systolic index was normal in 9 of the 13 patients preoperatively. After a shunt procedure, left ventricular end-diastolic volume and systolic index increased significantly in all four patients, and ejection fraction improved greatly in three of the four. After corrective surgery, left ventricular end-diastolic volume and left ventricular systolic index returned to the normal range and left ventricular ejection fraction improved. These findings suggest that patients with tricuspid atresia have impaired left ventricular function.

Cardiac Volume↗
PubMed1981

Interaction of 1-(5-phospho-beta-D-arabinofuranosyl)-5-substituted-uracils with thymidylate synthetase: mechanism-based inhibition by 1-(5-phospho-beta-D-arabinosyl)-5-fluorouracil.

A number of 1-(5-phospho-beta-D-arabinosyl)-5-substituted-uracils (ara-UMP's) have been examined as inhibitors of dTMP synthetase. As reversible inhibitors, all were substantially less potent than their 2'-deoxyribosyl counterparts. In the presence of 5,10-methylenetetrahydrofolate (CH2-H4folate), ara-FUMP caused a first-order, time-dependent inactivation of the enzyme. At 0 degrees C, kinetic studies indicated a reversible Kd of 3.6 micro M for the ara-FUMP-CH2-H4folate complex, and k = 0.22 min-1 for the subsequent inactivation. Spectral studies of the complex and its behavior toward protein denaturants demonstrate that its structure and stoichiometry are directly analogous to those which have previously been described for FdUMP. The significance of this finding with regard to prodrugs of ara-FU and the potential of ara-FU as a chemotherapeutic agent are discussed.

Arabinonucleotides↗
PubMed1981

Distribution of protein I, a synapse-specific phosphoprotein, and adenylate cyclase in the rat spinal cord.

The longitudinal and transverse distributions of the synapse-specific phosphoprotein Protein I and adenylate cyclase in the rat spinal cord were studied. Protein I was found to be enriched in all cervical and midlumbar (L3-L5) segments, and sparse in midthoracic and sacral segments. Adenylate cyclase activity was high in all cervical and lumbosacral segments, and low in mid-thoracic segments. Cross sectionally, both Protein I and adenylate cyclase were more enriched in the dorsal half than in the ventral half in the various segments studied. The similar topographical distributions of Protein I and adenylate cyclase in the spinal cord support the idea that adenylate cyclase may be intimately associated with Protein I in the nervous system, and could thereby regulate the state of in vivo phosphorylation of Protein I through formation of cyclic AMP.

Adenylyl Cyclases↗