Thomas L Williams | PubMed Health

PubMed2006

Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

Fatty Acids, Unsaturated↗

PubMed2005

Delivering distance training to rural health care professionals.

This article reports on a project that delivers distance training to rural health care professionals. Traveling to provide training on information-seeking skills to rural health professionals is time consuming and costly. In addition to face-to-face training, the University of South Alabama Biomedical Library's SAMNet project seeks to deliver multimedia training to rural health care professionals. The project uses information technology to package training courses combining PowerPoint slides and video instructions. This article describes the rationale, training module design and development, and the information technology and software used in the project. Multimedia packaged distance training courses provide a practical alternative to on-site training for rural health care professionals. It enables librarians to provide training without traveling long distance, thus saving time and money. Additionally, rural health care professionals may access the modules at a time convenient to them and proceed at a pace suitable to their learning style.

Academic Medical Centers↗