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Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.

Thomas L Williams·Margarida M L M Vareiro·A Toby A Jenkins
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Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

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Fatty Acids, UnsaturatedFluorescent DyesLipid BilayersPhospholipases APhospholipidsSensitivity and SpecificitySpectrometry, FluorescenceSurface Plasmon ResonanceSurface PropertiesTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Thomas L Williams, Margarida M L M Vareiro, A Toby A Jenkins. 2006-07-18. Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.. https://doi.org/10.1021/la060853q

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