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Biomedical subjects

U Lippi

Publications and source records attributed to U Lippi.

At least 37 records · Page 2Linked to original sources
PubMed1986

Application of flow-through cytochemistry utilizing the Technicon AutoSlide.

A fully automated procedure is described for combined stains of reticulocytes and other blood cells on whole blood smears. For this study, the Technicon AutoSlide, a device designed to operate synchronously with Technicon D/90 or H6000 systems to produce whole blood smears suitable for microscopic examination at the rate of 90 slides/hour, was utilized. Whole blood was mixed for 5 min with a new methylene blue solution in four mixing coils, smeared, and stained with Wright's stain. Preparations were mounted with an acrylic monomer that preserved stained smears indefinitely. The fully automated procedure is rapid standardized, and particularly suitable for use in large hematology laboratories.

Autoanalysis↗
PubMed1985

Mean platelet volumes: facts or artifacts?

Blood samples collected after venipuncture and skin-puncture from a series of ten healthy volunteers were used to determine platelet counts, mean platelet volumes (MPVs), and platelet distribution width (PDW). Measurements were performed on untreated venous whole blood and on whole or diluted samples, with or without anticoagulants. In this study, platelet indices were significantly different in untreated venous whole blood in comparison with blood from skin puncture and whole blood with anticoagulant.

Blood Platelets↗
PubMed1984

Catalytic activity of neutrophil and eosinophil peroxidase. Cytochemical and biochemical study in total hereditary myeloperoxidase deficiency.

We studied myeloperoxidase and eosinophil peroxidase activity in healthy subjects and in four totally myeloperoxidase deficient subjects using cytochemical and biochemical methods. Cytochemical tests demonstrated a pH dependent, enzymatic differentiated affinity of myeloperoxidase and eosinophil peroxidase for the chromogens used. The eosinophil peroxidase/myeloperoxidase ratio varied from 1.5 to 4. Biochemical assays, using MBTH-AA or o-dianisidine methods, produced eosinophil peroxidase/myeloperoxidase ratios of 1.98 and 1.72.

Eosinophils↗
PubMed1983

Clinical chemical indexes and severity of coronary atherosclerosis.

The present study measured triglycerides (TG), total cholesterol (TC), HDL-cholesterol (HDL-C), LDL and LDL/HDL-C, TC/HDL-C and HDL-C/TC. 100 ratios in 261 men and women undergoing coronary arteriography. The results show that TG, TC, HDL-C and LDL levels are not related to the severity of coronary artery disease (CAD). The LDL/HDL-C, TC/HDL-C and HDL/TC.100 ratios are statistically related to the severity of CAD. The LDL/HDL-C ratio appeared to be the most sensitive index for reflecting CAD of mild severity with a predictive value of 83.3% at 76.6% specificity and at 77.7% sensitivity (cut-off: 3.20). Average values of LDL and HDL-C alone are not reliable indices of mild severity CAD.

Adult↗
PubMed1983

More on the increase of hemoglobin A1 in chronic renal failure: the role of acidosis.

In chronic renal failure both Hb A1 and Hb A1c levels have been reported to be elevated. In order to investigate the causes of such increase we measured Hb A1 (cation-exchange chromatography), blood urea nitrogen, arterial blood pH and plasma bicarbonate in 40 patients with chronic renal failure receiving conservative treatment, 45 uremic patients on intermittent hemodialysis and 60 subjects with normal renal function. In patients with chronic renal failure Hb A1 highly correlates with arterial blood pH and plasma bicarbonate, thus emphasizing the major role played by uremic acidosis in the increased Hb A1 fraction in chronic renal failure.

Acidosis, Renal Tubular↗
PubMed1983

Origin of glycosylated hemoglobin A1 in chronic renal failure.

In chronic renal failure both HbA1 and HbA1c levels have been reported to be elevated. In order to investigate the causes of such increase we measured HbA1 (cation-exchange chromatography), blood urea nitrogen, arterial blood pH, plasma bicarbonate, phosphatemia, serum iron and serum ferritin before dialysis in 60 uremic patients receiving long term hemodialysis. The increased levels of HbA1 do not correlate with glucose intolerance, phosphatemia, blood urea nitrogen, time averaged concentration of urea, serum iron and serum ferritin. On the contrary the presence of a highly significant correlation between HbA1 and arterial blood pH (p less than 0.001) and between HbA1 and plasma bicarbonate (p less than 0.001) seems to emphasize a major role for acidosis in increasing the HbA1 levels in uremic patients on long term hemodialysis.

Acidosis↗