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U Lippi

Publications and source records attributed to U Lippi.

At least 55 records · Page 3Linked to original sources

Automated colorimetric method for the determination of serum gamma-glutamyl transpeptidase utilizing the Technicon SMAC alkaline phosphatase flow channel.

An automated procedure for the determination of serum gamma-glutamyltranspeptidase using Technicon SMAC alkaline phosphatase flow channel has been developed. The flow variants are insignificant. We use L-gamma-glutamyl-3-carboxy-4-nitroanilide as a very soluble substrate. With the dyalisis procedure, hemoglobin and bilirubin do not interfere. By employing dialysis, the need of blank corrections is therefore eliminated. The procedure can been run at 130 determinations per hour with insignificant carryover and satisfactory precision.

1-Carboxyglutamic Acid↗

[Simple rapid and economical procedure for agarose electrophoretic separation of serum lipoprotein fractions].

A detailed description is given of a rapid, simple and economic technique for the electrophoretic migration on agarose gel of serum lipoproteins. The albuminated agarose is spread hot on pieces of normal radiological plates even if already used. The reservoir for the serum is obtained by putting in the agarose while still hot a series of small metal bars. The moulds are removed with a small magnet as soon as the gel is formed. Plates prepared for 6-7 lipidograms can comfortably be kept for as long as 20-30 days. For stored plates ready for use, the analytic procedure does not require more than 40-45 minutes. The results can be considered altogether satisfactory.

Electrophoresis↗