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Biomedical subjects

V A Prianishnikov

Publications and source records attributed to V A Prianishnikov.

At least 37 records · Page 2Linked to original sources
PubMed1978

[Cytospectrophotometric study of GABA-transaminase in the rat cerebellar cortex].

Modification of the histochemical method for detection of GABA-transaminase activity is suggested. Optimal concentrations of the substrates and cofactors are chosen on the basis of kinetic study of the enzymatic reaction in the cryostat sections of the rat cerebellar cortex by the quantitative microspectrophotometric method. The method is intended for the quantitative histochemical analysis of GABA-transaminase activity in the brain sections.

4-Aminobutyrate Transaminase↗
PubMed1978

[Effect of combined administration of estradiol and progesterone on the mitotic cycle of the epithelial tissues of the reproductive tract of ovariectomized white rats].

The duration of stages of the cell cycle in the uterine and vaginal tissues of ovariectomized rats, treated with estradiol and estradiol-progesterone, was estimated using the labeled mitosis method. The joint treatment shortened G2 period in epithelial tissues. In the uterine epithelial tissues of estradiol-progesterone treated rats, the duration of S period was prolonged. In all tissues, progesterone stimulated the entry of cells into a second round of DNA synthesis. The estrogen-gestagen treatment inhibited the movement of vaginal epithelial cells from basal to superficial layers.

Animals↗
PubMed1977

[Modeling the cell cycle of complex populations].

A model of cell cycle kinetics of complex populations is developed, by using systems of difference equations, and simulated on the computer. This model provides theoretical curves of labeled mitoses (PLM) percentage, labeling index and grain count histograms for different time intervals after 3H-thymidine administration. The PLM curve corresponding to a mixed population is a weighed sum of PLMs for populations, comprising the mixture, the weights being determined by parameters of populations. The model appeared to give a good agreement with the PLM curves obtained elsewhere.

Autoradiography↗
PubMed1976

[Effect of the dose of estradiol and long-term administration of the hormone on the proliferative activity of the epithelium of the cavity, glandular epithelium and stroma of the white rat uterus].

The duration of S-sphase of the cell cycle in uterine epithelial and stroma tissues of ovariectomised rats, treated with two doses of estradiol-17 beta for 4 days, was estimated by the double labeling technique using H3 -and C14-thymidine. Estrogen treatment induced cyclical changes of S-phase duration in the uterine cell compartments, with maximal values at 72 and 24 hours for 1mcg and 1 mg of estradiol. resp. The high dose of estradiol induced five-fold reduction of the mitotic and labeling indices in luminal epithelium in contrast to the effect of the low dose. In the rat glandular epithelium and stroma cells mitotic and labeling indices are not influenced by eith of the estrogen dosis.

Animals↗
PubMed1976

[Glycogen level in endometrial cancer].

Studies on glycogen metabolism in tissues of endometrial cancer with various morphological structure have indicated that in most cases irrespective of the level of differentiation endometrial tumors contain no glycogen and show a slight activity of glycogensynthetase, phosphorylase and oxido-reductive enzymes. However, among the tumors under study there may be cases, which are characterized by a high content of glycogen and enzymes related with its metabolism. In these patients endometrial cancer was preceded by persistant disorders in the menstrual cycle. The relation between two types of the glycogen metabolism in endometrial cancer and the state of endogenous hormonal background is discussed.

Aged↗
PubMed1976

[A microphotometric study of the kinetics of the histochemical reaction to lactate dehydrogenase].

The aim of the work--to ascertain and substantiate concentrations of the main components of the incubation medium used for identification and quantitative evaluation of the activity of lactate dehydrogenase in cryostat sections. Studies were conducted using cryostat sections of the rat's liver as thick as 5, 10, 15 mcM with concentration in the incubation medium of lactate 25--125 mM, NAD 2--12 mM, nitrobleu and tetranitrobleu tetrazoles 0.3--5 mM, and also under conditions with addition of pyruvate in concentrations from 0.3 to 100 mM. The cytospectrophotometric method was used for evaluation of kinetic parameters of the histochemical reaction. The data on the dependence of the rate of tetrazole reduction upon the concentration of substrate, cofactor, thickness of the section and the level of the end product of the pyruvate reaction in the incubation medium were obtained. The main kinetic parameters of the enzymatic reaction in liver sections--value of Michaelis' constant and the concentration of the substrate at which the maximal rate of the reaction was observed--were calculated. Recommendations for selection of an optimal composition of the incubation medium, ensuring the strictly quantitative evaluation of the activity of an enzyme in cryostate sections are suggested.

Animals↗
PubMed1976

[A quantitative histochemical study of alkaline phosphatase isoenzyme kinetics in the thyroid gland, kidney and small intestine of white rats].

Microphotometric determination of alkaline phosphatase isoenzyme patterns in morphological structures of rat thyroid gland, kidney and small intestine were performed, using different concentration of L-levamisol and temperature-dependent enzyme inactivation. It was concluded that he constant of inhibition and degree of thermostability are closely connected with heterogeneity of alkaline phosphatase isoenzyme pattern in the organ.

Alkaline Phosphatase↗
PubMed1976

[Electron microscopic study of glycogen granules in the epithelial cells of human endometrial glands].

At the early secretory stage of the menstrual cycle part of glycogen granules in the human endometrial glandular cells are limited with threelayered membranes 6--9 nm thick. Transitional forms between the above mentioned granules and vesicles derived from the Golgi cisterns were found. On moving away from the Golgi apparatus the density of these granules with the membranes disappearing, thus suggesting that the Golgi apparatus of the human endometrial glandular cells may give rise to beta-particles of glycogen, whose polysaccharid nature is confirmed by amylase digestion.

Adult↗
PubMed1975

[Device increasing the capabilities of the MUF-5 microspectrophotometer].

Technical data for coordination of the device electric scheme and the MUPH-5 scheme (both with and without the integrator MIU-2) are presented together with a following computer analysis. The device enables to record discrete changes of optical density on the scanning pathway thus giving an advantage in application for quantitative analysis of histochemical enzymatic reaction as compared with the graphic registering.

Electronic Data Processing↗
PubMed1975

[Histochemical study of the enzymes of glycogen metabolism in the human endometrium during the normal menstrual cycle].

The content of glycogen in the human endometrium is dependet on the activity of enzymes involved in processes of synthesis and dissociation of glicogen: glycogen-synthetase, phosphorilase and glycogen-6-phosphatase. The activation of "D" form of glycogensynthetase dependent on glucose-6-phosphate and the increase of the glycoogen quantity in the endometrium glands epithelium is observed in the period of growing gestagenic function of the ovaries. At the end of the secretory phase there occurs activation of the glycogen dissociation enzme-phosphorilase which goes in parallel with abrupt inhibition of the glycogensynthetase activity and disappearance or glycogen from the gland epithelium. The inhibition of glycogen synthetase is conditioned by a decreased level of intracellular glucose-6-phosphate which appears due to activation of glucose-6-phosphate in this period.

Endometrium↗
PubMed1975

[A histochemical study of the effect of progesterone on the carbohydrate metabolism enzymes of different morphologic forms of endometrial cancer].

The histochemical method was used for determining glycogen, phosphorylase, glycogensynthetase, and glucose-6-phosphatase in order to evaluate the effect of progesterone on different morphological forms of cancer of the endometrium in man. On the basis of histochemical analysis of changes in the carbohydrate metabolism in 29 cases of cancer before and after treatment with progesterone a conclusion is drawn that sensitivity of tumours of the endometrium to hormonotherapy only partially depends upon the degree of their morphological differentiation.

Adenocarcinoma↗