Biomedical subjects

V Ahnonkitpanit

Publications and source records attributed to V Ahnonkitpanit.

6 recordsLinked to original sources

PubMed2003

Mouse blastocyst vitrification compared with the conventional slow-freezing method.

To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrification and slow-freezing were 68.33 and 65.52 per cent (p = 0.89), whereas hatching rates were 51.22 and 44.74 per cent, respectively (p = 0.64). Therefore, vitrification of blastocyst-stage-embryos may be a useful, economic method for freezing the excess blastocysts in some centers where blastocysts are routinely transferred.

Animals↗

PubMed2001

Correlation between human follicular diameter and oocyte recovery, metaphase II oocytes and fertilization rate in intracytoplasmic sperm injection programs.

This prospective study was undertaken to establish the correlation between the follicular diameter, oocyte recovery, metaphase II (MII) oocyte, and fertilization rate in intracytoplasmic sperm injection (ICSI) programs. Thirty one patients undergoing 31 ICSI cycles from August 1998 to January 1999 at King Chulalongkorn Memorial Hospital were studied. The patients were stimulated with either short or long GnRHa portocol. During transvaginal aspiration of follicles, follicular diameters were measured. The maturation stage of oocytes and fertilization rate were evaluated. Four hundred and seventy eight follicles were measured and classified into 3 groups, group A (<10 mm), group B (10-14 mm) and group C (>14 mm). The oocyte recovery rate and MII oocytes were different between groups A, B, and C but the fertilization rate was not different. In conclusion, the follicular diameter correlated with oocyte recovery and oocyte maturation but not with the fertilization rate in ICSI programs. The quality of embryos was lower in oocytes from small follicles.

Adult↗

PubMed2001

Effects of contaminated endometriotic contents on quality of oocytes.

The mechanism of infertility associated with endometriosis is poorly understood. There is evidence supporting that women with ovarian endometriosis have a lower pregnancy rate than women with peritoneal lesions only. This study aimed to evaluate the effects of endometriotic contents contamination while retrieving oocytes on oocytes' quality. Thirty-eight infertile patients with endometriotic cysts from January 1993 to June 2000 were enrolled in this study. There were no statistically significant differences among the quality of oocytes and embryos from the contaminated, non-contaminated, and control group. However, the fertilization rate and pregnancy rate were impaired by the contamination of endometriotic contents. We conclude that ovarian endometriosis should be treated before starting in vitro fertilization program in order to increase the pregnancy outcome.

Adult↗

PubMed1999

A study failing to determine significant benefits from assisted hatching: patients selected for advanced age, zonal thickness of embryos, and previous failed attempts.

PURPOSE: Pregnancy and implantation rates after mechanical assisted hatching (AH) in patients aged > or = 38 years, with embryos > or = 15 microns in zonal thickness and two or more failed attempts, were assessed at two infertility centers using fresh and frozen embryo transfer (FET) cycles. METHODS: AH was performed on 3-day-old embryos. Spare embryos cryopreserved at the two-pronucleus stage were subjected to AH after 2 days of culture and transferred to artificially prepared uteri. RESULTS: In fresh cycles, no significant differences in pregnancy rates (clinical and ongoing) and implantation rates were observed between the AH and the controls for all three selected patient groups (Centers 1 and 2). In FET cycles, AH tended to give poor results for > or = 38 year olds (clinical pregnancy rates of 0 and 5.0% with AH vs 13.3 and 16.7% for controls at Centers 1 and 2, respectively). With AH, embryos with thick zonae implanted to the same extent as those in the control group and achieved pregnancies for patients with multiple failures (four to six attempts for some) in both fresh and FET cycles. CONCLUSIONS: AH failed to show significant benefits in all three patient groups. A larger study group may confirm the effects of AH on frozen/thawed embryos and outcomes for multiple failure cases.

Abortion, Spontaneous↗

PubMed1999

Role of egg sulfolipidimmobilizing protein 1 on mouse sperm-egg plasma membrane binding.

We have shown that sperm sulfolipidimmobilizing protein 1 (SLIP1, molecular mass of 68 kDa), a sulfogalactosylglycerolipid (SGG)-binding protein, is significant in sperm-zona pellucida (ZP) interaction. The objective of this study was to localize SLIP1 on the egg and determine its role in gamete interaction. Immunofluorescence and immunoprotein A gold electron microscopy localized SLIP1 to the egg plasma membrane. In vitro gamete binding, using zona-free eggs preincubated with antiSLIP1 Fab before coincubation with sperm, showed a significant, dose-dependent decrease in sperm-egg plasma membrane binding. Similar results were obtained when affinity-purified antiSLIP1 IgG was used for egg pretreatment. The significance of egg SLIP1 in sperm-egg plasma membrane binding was further demonstrated by a decrease (36-52%) in in vitro fertilization when zona-intact eggs were pretreated with antiSLIP1 IgG. Since SLIP1 has been shown to bind SGG in vitro, we investigated the possibility that sperm SGG may participate in sperm-egg plasma membrane binding through egg SLIP1. Pretreatment of sperm with antiSGG Fab prior to coincubation with zona-free eggs resulted in a dose-dependent decrease in sperm-egg plasma membrane binding. Collectively, these findings strongly suggest a role for egg SLIP1 in sperm-egg plasma membrane interaction, which may be through its binding to sperm SGG.

Animals↗

PubMed1996

Bilateral tubal pregnancy following in vitro fertilization and embryo transfer.

We have presented a case of bilateral tubal pregnancy following in vitro fertilization and embryo transfer. The most likely cause, in our opinion, is 'spray effect' which was discussed. The diagnosis of ectopic pregnancy must always be kept in mind in IVF&ET program, due to its high incidence of occurrence compared to natural conception. Although the incidence of bilateral tubal pregnancy is not high, one should identify the opposite adnexa when tubal pregnancy is diagnosed in an IVF&ET patient. Early diagnosis is essential for prevention of significant maternal morbidity and mortality.

Adult↗