Mouse blastocyst vitrification compared with the conventional slow-freezing method.

W Tharnprisarn; S Suwajanakorn; W Sereepapong; K Pruksananonda; W Boonyakasemsanti; P Virutamasen; V Ahnonkitpanit; D Chompurat; P Numchaisrika

PubMed · 12948262

Mouse blastocyst vitrification compared with the conventional slow-freezing method.

W Tharnprisarn·S Suwajanakorn·W Sereepapong·K Pruksananonda·W Boonyakasemsanti·P Virutamasen·V Ahnonkitpanit·D Chompurat·P Numchaisrika

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Abstract

To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrification and slow-freezing were 68.33 and 65.52 per cent (p = 0.89), whereas hatching rates were 51.22 and 44.74 per cent, respectively (p = 0.64). Therefore, vitrification of blastocyst-stage-embryos may be a useful, economic method for freezing the excess blastocysts in some centers where blastocysts are routinely transferred.

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Animals Blastocyst Cryopreservation Embryo Transfer Female Male Mice Mice, Inbred ICR Time Factors Journal Article

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W Tharnprisarn, S Suwajanakorn, W Sereepapong, K Pruksananonda, W Boonyakasemsanti, P Virutamasen, V Ahnonkitpanit, D Chompurat, P Numchaisrika. 2003. Mouse blastocyst vitrification compared with the conventional slow-freezing method.. https://pubmed.ncbi.nlm.nih.gov/12948262/

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