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V Basaca-Sevilla

Publications and source records attributed to V Basaca-Sevilla.

28 records · Page 2Linked to original sources
PubMed1978

Search for heat-labile enterotoxigenic Escherichia coli in humans, livestock, food, and water in a community in the Philippines.

Environmental sources of heat-labile enterotoxigenic Escherichia coli are unknown. The feces of 1,086 inhabitants (approximately 5%) of a small town in the Philippines, 28 pigs, and 10 water buffalo were cultured for enteric bacterial pathogens. Twenty-seven persons harbored pathogenic bacteria: five individuals had enterotoxigenic E. coli, 11 Salmonella species, nine Vibrio parahaemolyticus, one Shigella boydii, and one nonagglutinable Vibrio. Enterotoxigenic E. coli were isolated from two of 28 pigs and from one of 10 water buffalo. Cultures of 26 pieces of beef, 25 pieces of pork, and 52 leafy vegetables obtained from a community market failed to grow enterotoxigenic E. coli. None of 47 samples of contaminated surface water contained this pathogen. Serotypes of human and animal strains of enterotoxigenic E. coli were different, although E. coli O78:H12 isolated from a pig has previously been incriminated in human diarrheal disease. In this limited survey of a Philippine community, enterotoxigenic E. coli were isolated from humans and livestock. The possibility that enterotoxigenic E. coli infections are zoonotic warrants further investigation.

Animals↗
PubMed1977

Biomedical survey in North Samar Province, Philippine Islands.

A biomedical survey was carried out in North Samar Province, Philipines to update information on the prevalence of parasitic and other infectious diseases. A total of 1,394 stool specimens, 1,859 blood smears and 1,274 sera were collected from persons living in 8 barrios. Stools were examined for intestinal parasites, bloods smears for malaria and filariasis and sera tested for antibodies to Schistosoma japonicum, Entamoeba histolytica, Toxoplasma gondii, influenza A and B, and Japanese encephalitis virus. The prevalence rates for intestinal parasites were: Trichuris trichiura 90%, Ascaris lumbricoides 78%, hookworm 65%, Schistosoma japonicum 15%, Strongyloides stercoralis 1%, Entamoeba coli 16%, Endolimax nana 6%, entamoeba histolytica 5%, Giardia lamblia 3%, Entaemoeba hartmanii 1%, Chilomastix mesnili 1%. No malaria was found but microfilariae of Wuchereria bancrofti were detected in 4% of the blood smears; the MfD50 was 12.9. The circumoval precipitin test (COPT) was used to detect antibodies to Schistosoma japonicum and 65% of 994 sera was considered positive. The indirect hemagglutination test (IHA) was used for detecting antibodies to Entamoeba histolytica and Toxoplasma gondii and 5% and 3% of 1,274 sera tested were positive at titers equal to or greater than 1:128 and 1:256, respectively. Hemagglutination inhibition tests (HI) were used to detect antibodies to Influenza A2HK68, Influenza A2HK68, Influenza B2T62 and Japanese encephalitis virus and 72%, 12% and 78%, respectively, of 1201 sera were considered positive at titers equal to or greater than 1:20.

Adolescent↗
PubMedDate not supplied

Longitudinal studies of children born to HIV-1 antibody positive Filipino commercial sex workers (CSW): diagnostic dilemmas.

Fifteen term babies born to 12 HIV-1 antibody positive Filipino CSW have been monitored for signs and symptoms of HIV-1 infection. Eleven babies were enrolled in the study within the first 6 months after birth; 4 others were enrolled at 4, 9, 11 and 21 months of age respectively. Every 3 months after enrolment, each baby received a physical examination, serum was tested for HIV-1 antibodies and p24 antigen and peripheral blood mononuclear cells were cultured for isolation of virus. After a mean follow-up period of 39.3 months (range 7-72 months), virus isolation and serum p24 antigen assays confirmed that 2 babies have been infected with HIV-1. If the 4 babies less than 18 months of age were excluded, the vertical transmission rate was 18.2%. Seven babies who have been monitored for a minimum of 25 months (range 31-60 months) lost their maternal antibodies but 6 of them subsequently developed indeterminant Western blots (WB); reactivity to p24 and/or gp120/ 160 but no reactivity to gp41. Of the remaining 6 babies, still less than 25 months of age (range 7-24 months), 2 lost their maternal antibodies within one year. The other 4 continued to recognize either p24 or gp120/160 well after the accepted 15-month period for loss of maternal antibody. Although a diagnosis could not be established upon the basis of these laboratory findings, clinical observations (failure to thrive, anergy, persistent generalized lymphadenopathy and recurrent pneumonias) mimicked HIV-1 infection. However, because these clinical features are common among many babies in the developing world, their usefulness in supporting a diagnosis of perinatal HIV-1 infection is limited.

Adolescent↗