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Biomedical subjects

V Bozdĕch

Publications and source records attributed to V Bozdĕch.

At least 73 records · Page 4Linked to original sources
PubMed1977

Rheumatoid arthritis and collagen antibodies.

By using the complement fixation test, antibodies against collagen were examined. Pronase-solubilized insouble collagen served as antigen (20 degrees, 24 hours, collagen: enzyme ratio 10:1). Of 79 patients suffering from rheumatoid arthritis, the presence of collagen antibodies was demonstrated in 8 cases. 56 patients with degenerative joint disease served as the control group, in which collagen antibodies were detected in only one single patient.

Animals↗
PubMed1977

[Radial complement fixation test and convex lens method for the determination of antibodies in tissues].

The radial complement fixation test has to be made in forms in 1.4% layer of agar in physiological saline in a Petri dish. These forms have to be of the same thickness as the reaction itself, usually about 1 mm. In the first layer, it is recommended to mix antigen with complement and sheep erythrocytes in agarose and this mixture is to be poured over the tissue fragments examined. Following the primary incubation (at room temperature overnight), another mixture of haemolysin in agarose is poured over the first layer in form of optical convex lenses. The haemolytic phase is performed at room temperature, too. The complement is titrated together with the optimal amount of antigen and a drop of positive serum is placed in the well just in the centre of the first layer. In this way an optimal dilution of complement is detected, which is capable of producing a full hemolysis outside the zone of the positive serum diffused during the first, primary incubation period, whereas the zone itself remains untouched with the haemolytic activity of complement.

Animals↗
PubMed1976

[Titration of antistreptolysin and control of its specificity in respect of percipitation reactions (author's transl)].

A method for the titration of antistreptolysin O (ASLO) is described that is assumed to be more rapid than the methods applied thus far. The authors are drawing attention to the non-specificity of this method and propose to use PRESLO which is easy to prepare and admits of quantitative evaluation, for a control of this non-specificity. Correlation between the two reactions were established by the authors in 2137 sera which was expressed mathematically and made subject to subsequent statistical evaluation.

Antistreptolysin↗
PubMed1976

[Intradermal toxoplasmin tests in Nigeria].

The occurrence of the positivity of an intracutaneous test with Toxoplasmin was followed among a normal urban population of Kaduna (men and women being included). The positivity of males showed a higher frequency in comparison with that of females. The positivity of males however did not increase so fast with the increasing age, as that of females, where the difference between the last two age groups was found to be statistically significant.

Adolescent↗