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Biomedical subjects

V Cerný

Publications and source records attributed to V Cerný.

At least 19 recordsLinked to original sources
PubMed1995

[Anticardiolipin antibodies and oncofetal antigens in pregnant women].

The presence of antiphospholipid antibodies (APA) in the blood stream is associated with some reproductive disorders in women. In the serum of 84 pregnant women, using the Elisa method, anticardiolipin antibodies (ACA) were examined. The first group of 53 women was formed by women with pathological levels of at least one of the oncofoetal antigens (alpha-1-fetoprotein-AFP, human chorionic gonadotropin-HCG and trophoblast-specific beta-1-glycoprotein- SP1). The second group of 31 pregnant women comprised women hospitalized on account of a risk pregnancy. In the first group women with reduced HCG were most frequently represented, where in 8.8% positive ACA were recorded. Of 11 patients with elevated HCG only in one the presence of ACA was detected. In nine pregnant women with reduced HCG and SP1 or AFP ACA positivity was found in 44.4%. In the second group ACA were detected in the serum of six pregnant women who had also pathological SP1 or HCG levels. From the results ensues that ACA could interfere with transduction signalizing processes in the cell and thus influence the synthesis of some proteins.

Adult↗
PubMed1995

[The transport system in the in vitro fertilization program].

The programme of in vitro fertilization helps at present successfully to resolve the problem of sterility in an ever increasing number of married couples. It is, however, a method which is very pretentious from the technical aspect, from the aspect of time and financial demands. Therefore in February 1994 with the Institute for the Care of Mother and Child in Prague-Podolí collaboration in the Transport IVF programme was started. In our clinic the first two stages of the IVF programme are implemented, i.e. stimulation of the ovaries and aspiration of the follicles. The patients of our centre for sterility are included in the programme, if the following indications are present: tubal factor 82%, idiopathic sterility 4%, the remainder being endometriosis, andrological factor and others. The majority of women were in the age group from 30-35 years. Stimulation is most frequently implemented by GnRh analogues and menopausal gonadotropins, aspiration of follicles by the transvaginal route with short analgosedation under so-called semi-ambulatory conditions. The aspirated follicular fluid is immediately stored in a special transport incubator G-812 of K-system Co., Denmark. Within 20 minutes it is taken by the patient's husband to the embryological laboratory of the Institute for the Care of Mother and Child in Prague-Podolí. Between February and December 1994 a total of 69 aspirations in 56 patients were made and 15 pregnancies were achieved. It is 23% pregnancies by transfer.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1994

[Antiphospholipid antibodies in women with reproductive disorders].

The authors examined in serum of 121 women, using the ELISA method, anticardiolipine antibodies (ACA) class IgG and IgM. The examined women were divided into a group of women with a normal pregnancy (n = 14), a group of women with imminent abortion during the first trimester (n = 10) and a group of women treated for prolonged periods on account of sterility (n = 97). In women with a normal pregnancy only in one a positive titre in the IgG class was recorded. In the group with imminent abortion a positive ACA titre was found in three patients (30%); in women with negative results of the ACA examination, pregnancy continued. In sterile women positive ACA tests were recorded in 14.5%.

Abortion, Threatened↗
PubMed1994

[Determination of antiphospholipid antibodies in serum using ELISA].

The authors compare two ELISA methods for the assessment of antiphospholipid antibodies, classes IgG and IgM, in serum: ELISA Pin Plate System ALPHA DIALAB Co. and the ELISA method developed in the Research Institute of Rheumatic Diseases. Both methods use cardiolipin as antigen. In the Pin Plate test the immunochemical reaction antigen/antibody does not take place at the surface of the pits of the microtitration plates but on the tip of the next plate. The results of examinations of antiphospholipid antibodies obtained by the tested methods are comparable, the Pin Plate test is quicker and more sensitive, but its price limits routine use.

Antibodies, Antiphospholipid↗
PubMed1993

Use of mild periodic acid-thiosemicarbazide-silver proteinate staining for ultrahistochemical demonstration of glycoconjugates in the human uterine epithelium.

The human uterine epithelium has been studied by means of mild (0.1, 0.01% respectively) periodic acid (PA) oxidation followed by thiosemicarbazide-silver proteinate staining. Distribution of silver deposits provided evidence for glycoconjugates and was in the case of oxidation with 0.01% PA selective for sialic acid (N-acetylneuraminic acid) complexes. The electron dense silver particles were detectable at the outer side of apical plasma membranes and on the inner surface of lysosomal membranes. Also the marginal zone of lipid droplets reacted regularly. Since the lower concentration of PA proves sialogycoconjugates selectively, the positive staining depicted not only those in the membrane compartments but also as a layer between the hydrophobic content and the hydrophilic cytoplasm. In the granular endoplasmic reticulum of the individual microvillous cells a special glycoprotein of paracrystalline organisation was detected. The functional significance of this substance remains unclear. Nuclear channel systems (NCS) are frequently observed in the epithelial cells in the uterine glands. Similar staining properties of the NCS and nuclear envelope support the hypothesis of its nuclear origin.

Endometrium↗
PubMed1993

[The short-bowel syndrome].

This study was designed to asses the clinical course of five children with a short bowel syndrome after massive intestinal resection during neonatal period. On the basis of their experience the authors analyze some properties that may be advantageous in this syndrome and discuss the most important factors, including enteral feeding at an early postresection stage, which influence a full development of the small bowel adaptive mechanisms. They point out that so called "short gut colitis", sometimes occurring during advancement of enteral nutrition, can be successfully treated by sulphasalazine. The authors come to the conclusion that the prognosis for newborn infants undergoing an excessive bowel resection is far well thanks to enhancement of the intestinal adaptation by a combination of a long-term parenteral nutrition with enteral feeding.

Female↗
PubMed1990

[Bilateral testicular tumors].

Bilateral testicular tumours account for cca 2-4% of all testicular tumours. In 1975-1989 at the Urological Clinic of the Faculty Hospital with Policlinic in Olomouc a total of 272 patients with testicular tumours were treated, incl. 6 with bilateral tumours which developed successively. The interval between the development of the tumours was on average 5.8 years. All patients survive 1-9 years after the diagnosis of the contralateral tumour.

Adult↗
PubMed1990

Occurrence of the tick Ixodes ricinus (L.) under the conditions of anthropopressure.

The authors report on their findings of the tick Ixodes ricinus under urban conditions of Prague. The concrete data were taken as a basis for a general scheme of possible occurrence of this species in various town parts, determined by the state of vegetation and presence of large mammals. Discussed are also conditions for I. ricinus maintenance in another type of anthropogenous landscape, the spoil banks resulting from brown coal exploitation.

Animals↗
PubMed1989

Immunofluorescence detection of porcine anti-zona pellucida antibodies in sera of infertile women.

Fifty three samples of blood sera of women from infertile couples were investigated for presence of antibodies to zona pellucida. Using the immunofluorescence test, antibodies reacting with porcine zona pellucida could be detected in sera of women with unexplained infertility and also in the sera of a control fertile group. In most cases these antibodies could be adsorbed by porcine erythrocytes, indicating that the erythrocyte-like antigens are present in the porcine zona pellucida, as was confirmed by electron microscopic studies.

Animals↗
PubMed1989

Treatment of advanced Hodgkin's disease with modified MOPP regimens. A long-term observation.

Fifty-three patients with advanced Hodgkin's disease, most of them previously treated, received 8 to 16 courses of modified MOPP regimens (nitrogen mustard replaced by trichlormethine in arm A, with addition of vinblastine to the 4-drug regimen in arm B, and alternation of three drugs--trichlormethine, vincristine, and prednisone--with probably non-cross resistant two drugs--vinblastine and procarbazine in arm C). Thirty patients (57%) achieved complete remission. Higher complete remission rate and longer survival was recorded in patients treated with 5-drug regimens (arms B and C) as compared to the 4-drug regimen (arm A), but the differences were not significant. Higher complete remission rates were observed in asymptomatic patients, females, and patients with lymphocyte predominance and nodular sclerosis subtypes of Hodgkin's disease. Besides expected short-term toxicity, 4 out of 30 complete responders developed secondary malignancies (two acute myeloblastic leukemias, one hepatocellular carcinoma, and one cerebellar astrocytoma). Several other patients had serious toxicity which could be attributed to chemotherapy. Twenty-eight percent of the patients has been alive 15 to 18 years since the start of this study.

Adolescent↗
PubMed1989

[Pyeloureterostomy in necrosis of the ureter in a transplanted kidney with a low ureteral fissure].

Duplication of the urinary pathways of a transplanted kidney makes the localization of the ureteral defect and its repair more difficult. The authors describe difficulties associated with detection of the site where urine escapes and with restoration of the continuity of the urinary pathways by pyelopyeloureterostomy in a female patient with transplantation of a kidney with low fissure of the ureter.

Female↗