[Reaction of hypochlorite-anion with unilamellar phosphatidylcholine liposomes by UV absorption spectrophotometry].
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Anions↗
Biomedical subjects
V E Formaziuk
Publications and source records attributed to V E Formaziuk.
[Interactions of various amino acids, dipeptides and glutathione with hypochlorite anion (CLO-)].
The specific features of interaction between sodium hypochlorite and various amino compounds were examined. It is shown that in the first reaction munite, methionine and glutathione are the most active in neutralizing hypochlorite anion, while histidine, glycine, alanine, dipeptides and taurine are less active. The chloroamino complexes formed by dipeptides and taurine are the most resistant.
Alanine↗
[Formation of water-soluble complexes between phosphatidylcholine ane methionine, carnosine, and glutathione in the presence of sodium hypochlorite].
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Carnosine↗
[Surface activity and wetting action of preparations of artificial tears].
The surface tension of dilute solutions of 14 artificial tears preparations and natural tears has been measured. Their wettability action on the paraffin surface has been estimated. The preparations with optimal wettability have been determined.
Ophthalmic Solutions↗
[A comparison of the antioxidative activity of carnosine by using chemical and biological models].
The difference in the efficiency of carnosine as an antioxidant was found to be explained both by the source of carnosine and the specificity of models used to achieve visualization. Commercial carnosine samples were contaminated with compound (s) absorbing at 255-332 nm. At the same time they possessed better antioxidant activity in the models with Fe2-induced peroxidation process. In the case of chemical models for generation of active forms of oxygen (several modifications of the Fenton reaction) or during burst of superoxide generation by leucocytes, the antioxidant effect of carnosine did not depend of the source of the compound under study.
Animals↗
[Diadenosine oligophosphates: metabolic pathways and role in regulating the functional activity of cells].
Recent data on the content and biological role of diadenosine oligophosphates in procaryotic and eucaryotic cells, animal tissues and organs are reviewed. The specific ways of their synthesis and dissociation are presented. The role of diadenosine oligophosphates in regulation of cell proliferation and as protectors from cells damage with cytotoxic agents in discussed. The perspectives uses of diadenosine oligophosphates in medicine have been examined. The role of a universal diadenosine oligophosphates system in regulation of cell biological activity is postulated.
Adenosine Triphosphate↗
[Interaction of myeloperoxidase and defensins with lipid monolayers].
Myeloperoxidase and defensin interaction with lipid mixtures of phosphatidylcholine and cholesterol or rat eye lens lipids were studied. The solubility of myeloperoxidase in 1-octanol and octanol was determined. It was shown that myeloperoxidase can be concentrated at the interface and is readily soluble in the membrane lipid phase. Defensins exhibit similar properties and increase, in addition, the cholesterol-rich monolayer tension. Possible consequences of highly cationic proteins adsorption on cholesterol-rich membranes are discussed.
1-Octanol↗
[Characteristics of chloramine complexes of carnosine with hypochlorite anion].
Carnosine interaction with CIO results in the formation of a stable chloramine complex. The binding of the whole bulk of hypochlorite to carnosine is completed within one minute of incubation. During subsequent 2-hour incubation no more than 15% of the chloramine complex is destroyed; this property of carnosine makes it similar to taurine. Unlike histidine and beta-alanine, glutathione rapidly interacts with hypochlorite. However, in contrast with these compounds and carnosine, glutathione does not form stable chloramine complexes with CIO. The putative role of myeloperoxidase in the development of senile human lens opacities is discussed.
Animals↗
[The use of carnosine in medical practice. Priorities: past and future].
The history of the discovery of curative effects of carnosine and its perspective applications in the clinical practice are reviewed. The molecular mechanisms of carnosine interactions with free oxygen radicals (hypochlorite anion, in particular) are considered.
Anions↗
[The role of myeloperoxidase, a product of the polymorphonuclear leukocytes, in the pathogenesis of cataract].
The capacity of myeloperoxidase which is the product of polymorphonuclear leucocytes to induce the lens opacity was studied in young and old rabbits. It was found that the injection of myeloperoxidase solution into anterior chamber of the eye causes the irreversible lens opacification in old rabbits, not in young ones. Light microscopy of the lens section has shown the following alterations: the local thickening of the anterior capsule, disorderly accumulation of epithelial cells, formation of so-called "bladder cells" under the lens epithelium. Changes in experimental eyes are typical for cataract.
Aging↗
[The interaction of taurine and beta-alanyl-L-histidine (carnosine) with hypochlorite anion (ClO-)].
Dipeptide carnosine and amino acid taurine have been found to actively interact with hypochlorite anion. Chloramine complexes obtained during this reaction were more stable in case of taurine. It is suggested that therapeutic effect of new Russian eye drops taufon and sevitin is due to neutralization of the reaction product hypochlorite anion catalyzed by myeloperoxidase.
Anions↗
[Formation of stable chloramine complex in interactions of carnosine and hypochlorite anion].
Nowadays carnosine is considered as a promising drug for different diseases. However, mechanism of its action has not been found yet. The study evaluated the interaction between carnosine and sodium hypochlorite. It is established that this reaction yields a stable chloramine complex which is likely to underlie the effect of the drug in cataracts.
Animals↗
[Myeloperoxidase interaction with mixed monolayers of lecithin and cholesterol].
Interaction between myeloperoxidase and mixtures of lecithin-cholesterol monolayers were investigated. Solubility of the enzyme in 1-octanol or in octanol were tested too. It is shown that myeloperoxidase demonstrates an ability to concentrate on phase limits. The enzyme well dissolves in lipid membrane phases. It is possible to assume that myeloperoxidase penetrates and accumulates in the eye lens.
Adsorption↗
[Chemiluminescence decrease of singlet oxygen in NaClO+H2O2 reaction in the presence of different anti-cataract drugs].
Singlet oxygen was produced in chemical reaction NaClO+ H2O2. Action of different well-known anti-cataract drugs on this reaction was studied. There is no doubt that the singlet oxygen chemiluminescence decreases in the presence of Catalin and Baineiting. Finnish Catachrom Ophthan, Vita iodurol (France) and Quinax (USA) have no such effect at all which may be a result of the interaction of these remedies with H2O2 and/or with NaClO.
Acridines↗
[Dynamics of alkaline hydrolysis of RNA oligoadenylates in hydrolysis of homogenates of the rat cerebral cortex and liver].
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Adenosine Monophosphate↗
[Immunochemical analysis of water-soluble antigens of the cornea].
Rabbits were immunized by the water soluble cow cornea antigens. The particle immunochemical identity between cow cornea antigens and cow lens, vitreous humor, aqueous humor, iris, choroid and retina was found in reaction of immunodiffusion in gel. Immune cross reactions between cow cornea antigens and human antigens of different tissue were absent. 9 antigens were indicated by immunoelectrophoresis in cow cornea. Three of them with gamma-mobility locate in epithelium, 2-with gamma-mobility--in endothelium and 4 (two with beta-mobility, and one of alpha 1-mobility and another alpha 2-mobility) in stroma. The possible role of different antigenic composition of cornea is discussed.
Animals↗
[Detection of myeloperoxidase in eye tissues of man].
Myeloperoxidase (MPO) is an enzyme which usually detected in leukocytes of peripheral blood. Its principal function is to produce bactericidal hypochlorite-ions in reaction of hydrogen peroxide with chlor ions. The present work deals with testing myeloperoxidase activity in different eye tissues. Evolution of MPO activity has been tested by means of dianisidine reaction and immunochemical identification in extracts. High activity of MPO was found for retina and lens of healthy men and elderly people with lens opalescence. It should be noted that cellular body, cornea, aqueous humor, vitreous humor do not manifest any noticeable activity. Iris has low activity.
Adult↗
[Decrease of singlet oxygen chemiluminescence by the presence of carnosine].
To test antiradical medicines effect the chemical production of singlet oxygen (NaClO + H2O2) was investigated. The quantity singlet oxygen chemiluminescence was decreased in the presence of Japanese Catalin and Chine Baineiting, antirheumatic Voltaren and less strong Finish Catachrome and Carnosine. American Quinax does not possess such an effect. One of the possible starting mechanisms causing different diseases (atherosclerosis, cataract etc.) is destruction of biomembranes by active forms of oxygen.
Acridines↗