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Biomedical subjects

V F Runova

Publications and source records attributed to V F Runova.

At least 19 recordsLinked to original sources
PubMed1992

[The fractional composition of immunoglobulin preparations studied by gel filtration on different carriers and by high-pressure liquid chromatography].

The fractional composition of immunoglobulin preparations produced by different manufacturing enterprises of this country has been studied by gel chromatography in columns packed with different carriers (Sephadex G-200 and ultragel AcA-34) and by high-performance liquid chromatography (HPLC). This study has revealed the nonstandard character of immunoglobulin preparations produced according to the same technological procedure (modified Cohn's method). The fractionation of immunoglobulins on different carriers with the use of different methods has yielded similar results confirmed by the statistical processing of the data. The results obtained in the study of the fractional composition of immunoglobulin preparations evidence that gel filtration with the use of ultragel and HPLC have greater resolving capacity in comparison with the method of gel filtration on traditionally used Sephadex G-200.

Chromatography, Gel↗
PubMed1992

[An evaluation of the quality of tetanus anatoxins by gel electrophoresis].

The study of tetanus toxoids obtained from different manufacturers in the USSR has shown that these preparations exhibit molecular heterogeneity. The method of gel filtration has made it possible to find out that tetanus toxoids from different manufacturers differ in the degree of their purification. The preparations produced by the manufacturing enterprises in Perm and Ufa have been found to contain considerably less ballast substances than the preparations produced in Moscow.

Calibration↗
PubMed1991

[A gel filtration study of the fractional composition of tetanus antitoxic sera].

The fraction composition of tetanus antitoxic sera has been studied by the method of gel filtration in columns packed with Sephadex G-200. Differences in the fraction composition of preparations manufactured by different enterprises have been revealed. The specific activity of antitoxic sera has been found to depend on the degree of their fragmentation.

Chromatography, Gel↗
PubMed1988

[Cytotoxic action of the chemical substances found as admixtures in medical immunobiological preparations].

The cytotoxic action (CTA) of chemical substances contained as admixtures in medical immunobiological preparations on human diploid cells has been studied. Such chemical substances as rivanol and merthiolate in admissible concentrations show the highest degree of CTA. The results obtained in this investigation indicate that different concentrations of chemical substances may produce equal CTA; thus, thiolate in toxic in a dose of 0.8 microgram/ml; the same CTA is produced by aluminium sulfate in a concentration of 500 micrograms/ml. Small doses of chemical substances, producing no explicit manifestations of the cytotoxic effect, may have latent CTA determined by additional methods of investigation. CTA may be manifested as lethal, sublethal and latent cell lesions. In working out regulations on the test for CTA it is expedient to indicate admissible residual amounts of chemical substances contained in finished medical immunobiological preparations, considering that these amounts must be incapable of producing CTA in cell cultures. The conclusion has been made on the expediency of denoting small amounts of chemical substances capable of producing latent CTA as tentatively tolerable doses.

Biological Products↗
PubMed1987

[Dependence of the specific activity of an immunoglobulin against tick-borne encephalitis on the degree of its fragmentation].

Immunoglobulin preparations against tick-borne encephalitis with the increasing content of Fab-fragments (from 16% to 45%) have been experimentally obtained. As revealed by testing these samples in vivo for specific activity (in the biological neutralization test), the preparations containing 16% of Fab-fragments show no perceptible decrease of specific activity; its sharp decrease (2-16 times) can be observed in preparations with a high degree of fragmentation (the content of Fab-fragments being equal to 30-45%).

Animals↗
PubMed1987

[Molecular composition of the fractions of immunoglobulin preparations studied by gel filtration].

The method of gel filtration in columns permitted the separation of aggregated fractions into polymers whose content did not exceed 10% and dimers, their content ranging from 3.6% to 22.11%. The preparations were also found to contain fractions of monomers and fragments, Fab-fragments being detected in 10 out of 20 batches under study (4.04-27.36%). The shelf life of all preparations did not exceed 8-12 months. The use of spectrophotometric techniques ensured obtaining the most objective results in the calculation of the percentage of fractions contained in immunoglobulin preparations. The evaluation of the molecular composition of immunoglobulin preparations by the method of gel filtration is conducive to the improvement of their quality.

Chromatography, Gel↗
PubMed1986

[Dependence of the specific activity of antitetanus immunoglobulin on the degree of its fragmentation].

Antitetanus immunoglobulin preparations with the increasing content of Fab-fragments (15, 30, 53%) have been obtained under specific experimental conditions. Tests for specific activity have revealed an insignificant decrease (13%) in this activity in the preparation containing 15% of Fab-fragments and its sharp drop in the preparations containing 30-50% of Fab-fragments. The specific activity of antitetanus immunoglobulin has been found to be related to the degree of its fragmentation.

Animals↗
PubMed1986

[Dependence of the specific activity of antistaphylococcal immunoglobulin on the degree of its fragmentation].

In vitro experiments have revealed no perceptible decrease in the content of antitoxic antibodies to alphastaphylolysin in the preparations of antistaphylococcal immunoglobulin, containing 13-50% of Fab-fragments. These data confirm the necessity of controlling the molecular composition of the preparations of antistaphylococcal IgG by physico-chemical methods with the aim to evaluate their quality, as the fragmented preparations are rapidly eliminated from a human body.

Antibody Specificity↗
PubMed1979

[Biological and chemical characteristics of allergen preparations from Neisseria (N. meningitidis, N. gonorrhoeae, N. perflava)].

Various methods of isolating allergen fractions from N. meningitidis, N. gonorrhoeae and N. perflava were tested. The biological activity of the preparation was found to depend on the method of its production, which determined its chemical composition. When gonococcal and meningococcal allergens and N. perflava allergen were used in skin tests, cross reactions were observed. Nevertheless, as the intensity and size of skin reaction was much greater when a homologous preparation was administered, it was possible to differentiate the presence of sensitization to a definite microbial species. Electrophoresis in acrylamide gel revealed the heterogeneity of allergen preparations. The ability of the preparation to induce skin reaction was not connected with its serological properties.

Allergens↗
PubMed1978

[Comparative study of allergenic fractions extracted from different strains of staphylococci].

Various methods for obtaining staphylococcus allergens were approbated. Biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. A method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be recommended for the preparation of staphylococcus allergen. Biological activity of the allergen in case of its approbation in the same dose by protein on laboratory animals and patients proved to be the same.

Allergens↗
PubMed1978

[Characteristics of the biological activity of staphylococcal allergens obtained from various strains laboratory animals].

The authors suggest an optimal scheme of guinea pig sensitization for the assessment of specific activity of staphylococcal allergens. On milliard cells of killed staphylococcal culture are injected to the animals into the paws of the limbs; two and a half weeks later an intradermal test with an allergen in a dose of 10 microgram by protein was made. Differences in the specific activity of staphylococcal allergens prepared of various strains were revealed. Biochemical properties of the strains proved to be of significance for the sensitization.

Allergens↗
PubMed1977

[Effect of chemical action on the biological activity of staphylococcal allergen].

The authors present the results of study of chemical action on the specific properties of staphylococcus allergen showing that deamination, iodation, and formalinization was accompanied by reduction of the allergenic capacity of the preparation; as to acetylation -- it produced an insignificant reduction of the allergen activity.

Acetylation↗
PubMed1975

[Fractionation of staphyococcal allergens on sephadex].

Allergens obtained from seven strains of staphylococcus cultures (with and without any toxigenic and hemolytic properties) were subjected to fractionation on Sephadexes. It was shown that allergens from strains 209, 3a, 977 contained one protein-polysaccharide, component with molecular weight of 600 000--900 000. Allergens from strains Wood-46, 150a, r-7, O15 were characterized by the presence of two protein-polysaccharide fractions with molecular weight of 800 000--900 000 (I) and 20 000--100 000 (II). There was revealed the influence of lyphilization on the separation of nucleic acids in the preparations not subjected to lyophilization; nucleic acid fractions were separated in gel filtration; no separation of nucleic acids from the protein-polysaccharide fractions followed lyophilization.

Allergens↗
PubMed1975

[Protein sorption with mineral colloids].

A study was made of the effect on sorption of the molecular weight of model proteins (ribonuclease with a molecular weight of 12 10(3), trypsin with a molecular weight of 24-10(3), bovine albumin with a molecular weight of 64-10(3) and gamma-globulin with a molecular weight of 160-10(3)) and dispersity of suspensions of aluminium hydroxide, aluminum phosphate and calcium phosphate used as biopreparation sorbents. The expediency of using phosphate and calcium phosphate used as biopreparation sorbents. The expediency of using for effective sorption of a definite area of sorption surface necessary and adequate for the distribution of protein macromolecules with the best degree of conformational liberty was revealed.

Adsorption↗