Biomedical subjects

V F Runova

Publications and source records attributed to V F Runova.

At least 37 records · Page 2Linked to original sources

PubMed1978

[Comparative study of allergenic fractions extracted from different strains of staphylococci].

Various methods for obtaining staphylococcus allergens were approbated. Biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. A method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be recommended for the preparation of staphylococcus allergen. Biological activity of the allergen in case of its approbation in the same dose by protein on laboratory animals and patients proved to be the same.

Allergens↗

PubMed1978

[Characteristics of the biological activity of staphylococcal allergens obtained from various strains laboratory animals].

The authors suggest an optimal scheme of guinea pig sensitization for the assessment of specific activity of staphylococcal allergens. On milliard cells of killed staphylococcal culture are injected to the animals into the paws of the limbs; two and a half weeks later an intradermal test with an allergen in a dose of 10 microgram by protein was made. Differences in the specific activity of staphylococcal allergens prepared of various strains were revealed. Biochemical properties of the strains proved to be of significance for the sensitization.

Allergens↗

PubMed1977

[Effect of chemical action on the biological activity of staphylococcal allergen].

The authors present the results of study of chemical action on the specific properties of staphylococcus allergen showing that deamination, iodation, and formalinization was accompanied by reduction of the allergenic capacity of the preparation; as to acetylation -- it produced an insignificant reduction of the allergen activity.

Acetylation↗

PubMed1975

[Fractionation of staphyococcal allergens on sephadex].

Allergens obtained from seven strains of staphylococcus cultures (with and without any toxigenic and hemolytic properties) were subjected to fractionation on Sephadexes. It was shown that allergens from strains 209, 3a, 977 contained one protein-polysaccharide, component with molecular weight of 600 000--900 000. Allergens from strains Wood-46, 150a, r-7, O15 were characterized by the presence of two protein-polysaccharide fractions with molecular weight of 800 000--900 000 (I) and 20 000--100 000 (II). There was revealed the influence of lyphilization on the separation of nucleic acids in the preparations not subjected to lyophilization; nucleic acid fractions were separated in gel filtration; no separation of nucleic acids from the protein-polysaccharide fractions followed lyophilization.

Allergens↗

PubMed1975

[Protein sorption with mineral colloids].

A study was made of the effect on sorption of the molecular weight of model proteins (ribonuclease with a molecular weight of 12 10(3), trypsin with a molecular weight of 24-10(3), bovine albumin with a molecular weight of 64-10(3) and gamma-globulin with a molecular weight of 160-10(3)) and dispersity of suspensions of aluminium hydroxide, aluminum phosphate and calcium phosphate used as biopreparation sorbents. The expediency of using phosphate and calcium phosphate used as biopreparation sorbents. The expediency of using for effective sorption of a definite area of sorption surface necessary and adequate for the distribution of protein macromolecules with the best degree of conformational liberty was revealed.

Adsorption↗

PubMed1974