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V I Bugrova

Publications and source records attributed to V I Bugrova.

At least 19 recordsLinked to original sources
PubMed1976

[Obtaining purified staphylococcal enterotoxin type B].

The authors elaborated a method of purification of staphylococcus enterotoxin, type B, consisting of three stages: the first stage--lyophilization and dialysis of the culture fluid against distilled water for 36 hours, the second stage--precipitation of dialyzed preparation with 2 volumes of ethanol at --15 degrees C, and the third stage--gel-chromatography of the enterotoxin preparation precipitated with alcohol on a column with Sephadex G-100 in a 0.015 M solution of NaCl (pH 7.0). Preparation isolated by the mentioned method possessed enterotoxic activity in a dose of 0.015 mg of protein per 1 kg of cat weight; by antigenic composition it represented a two-component system. In immunization of rabbits with a purified enterotoxin preparation an antiserum with the prevalence of antibodies to enterotoxin, type B, was obtained.

Enterotoxins↗
PubMed1975

[Effect of thermal treatment on the properties of staphylococcal enterotoxin type A].

Thermal treatment of staphylococcus enterotoxin of type A at the stage of the cultural fluid failed to influence the character of the enterotoxin purification under the given conditions. In both cases the amount of the removed nitrogen components constituted about 97%. The purified preparations of the staphylococcus enterotoxin of type A differed from one another by the data of electrophoresis and ultracentrifugation and also by the antigenic composition. Under the action of thermal treatment staphylococcus enterotoxin of type A changed its antigenic properties and lost some of its biological activity.

Animals↗