[Obtaining purified staphylococcal enterotoxin type B].

Iu V Ezepchuk; F S Fluer; V I Bugrova

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PubMed · 941595

[Obtaining purified staphylococcal enterotoxin type B].

Iu V Ezepchuk·F S Fluer·V I Bugrova

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Abstract

The authors elaborated a method of purification of staphylococcus enterotoxin, type B, consisting of three stages: the first stage--lyophilization and dialysis of the culture fluid against distilled water for 36 hours, the second stage--precipitation of dialyzed preparation with 2 volumes of ethanol at --15 degrees C, and the third stage--gel-chromatography of the enterotoxin preparation precipitated with alcohol on a column with Sephadex G-100 in a 0.015 M solution of NaCl (pH 7.0). Preparation isolated by the mentioned method possessed enterotoxic activity in a dose of 0.015 mg of protein per 1 kg of cat weight; by antigenic composition it represented a two-component system. In immunization of rabbits with a purified enterotoxin preparation an antiserum with the prevalence of antibodies to enterotoxin, type B, was obtained.

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Enterotoxins Methods Staphylococcus aureus English Abstract Journal Article

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Iu V Ezepchuk, F S Fluer, V I Bugrova. 1976. [Obtaining purified staphylococcal enterotoxin type B].. https://pubmed.ncbi.nlm.nih.gov/941595/

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