PubMed Health⌕ Search

Biomedical subjects

V I Votiakov

Publications and source records attributed to V I Votiakov.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[Formation of streptokinase-heparin complexes and their properties].

Streptokinase and heparin developed complex at the ratio 3.2:1, w/w, respectively, and neutral values of pH, which was registered by means of thin-layer chromatography and differential UV-spectroscopy. IR-, differential UV- and CD-spectroscopic methods showed that secondary and tertiary structures of streptokinase were altered in the complex. Streptokinase containing in the complex exhibited the higher thermostability at 50 degrees as compared with native protein. After intravenous administration of the complex into rabbits more distinct alterations were observed in thrombin time, blood clot lysis and in blood plasma fibrinolytic activity as compared with native streptokinase effect.

Animals↗
PubMedDate not supplied

[Pathogenesis of amyotrophic leukospongiosis reproduced in guinea pigs by retrobulbar infection].

Pathogenesis of amyotrophic leukospongiosis (ALSP) with a short incubation period induced in guinea pigs was studied. After retrobulbar inoculation, the unconventional ALSP virus disseminated both neurogenically (along the optic nerve) and hematogenically. At early stages of the disease the spleen appeared to play the leading role in multiplication and/or accumulation of the agent. The earliest morphological sign of the CNS involvement was vacuolation of motoneuron cytoplasm. The ALSP agent first affected the spinal motoneurons, then an ascending pathological process developed. In the terminal stage of the disease, the unconventional ALSP virus was also detected in visceral tissue, besides the CNS, spleen, and peripheral blood lymphocytes.

Animals↗
PubMedDate not supplied

[Physicochemical and biological properties of the agents isolated from a patient with amyotrophic leukospongiosis and monkeys with experimentally reproduced disease].

The paper describes isolation from the brain of a patient who died of amyotrophic leukospongiosis (ALSP) and from squirrel monkeys with experimentally induced disease of heretofore unknown agents whose reproduction in continuous cell lines (HEp-2, CV-1, Vero) was accompanied by a significant increase in the mitotic activity of cells and adsorption of human 0(1) and M. rhesus erythrocytes. The blood sera from patients with ALSP and a monkey with experimental disease were found to contain a factor specifically inhibiting the hemadsorption phenomenon and the increase of the mitotic activity in the infected cell cultures. Investigations of the properties of the isolates showed them to have a buoyant density of 1.18 g/cm3 in a 20-60% sucrose gradient, to incorporate radioactive precursors of RNA and protein synthesis, and to be highly resistant to the effect of some physical and chemical factors. The data obtained suggest that the isolates differ from all the presently known conventional viruses but have some similarity with etiological agents of slow infections and may be classified as unconventional viruses.

Animals↗
PubMedDate not supplied

[Reproduction of amyotrophic leukospongiosis in laboratory animals].

Amyotrophic leukospongiosis (ALSP) was produced in laboratory animals: hamsters, rabbits, guinea pigs. Guinea pigs were found to be a most suitable laboratory model for the study of ALSP pathogenesis with the longest incubation period. The data are obtained verifying the role of previously isolated unconventional virus in the ALSP etiology.

Animals↗
PubMedDate not supplied

[Characteristics of the etiological agent of human amyotrophic leukospongiosis].

Primary cultures of brain cells from a patient with amyotrophic leukospongiosis (ALSP) and animals with the experimentally reproduced disease yielded an agent which by its physicochemical and molecular biological properties was placed among nonclassical viruses, the agents of spongiform encephalopathies. The agent is small and resistant to a number of detergents, lipid solvents, oxidants, enzymes, UV irradiation, and heating. The role of the isolate in the development of ALSP is verified by experimental reproduction of the disease in guinea pigs inoculated either with a brain suspension from ALSP patient or with the purified and concentrated agent. The true nature of ALSP in experimentally inoculated animals was verified by clinical, morphological, and virological studies.

Animals↗
PubMedDate not supplied

[Isolation of influenza A viruses with a new antigenic formula from wild birds].

Three hemagglutinating agents were isolated from mixed pools of the viscera from black-headed gulls (Larus ridibundus) and robin (Erithacus rubecula) collected in the Byelorussian SSA and the Kaliningrad region of the RSFSR. Typing of the viruses by double immunodiffusion technique revealed antigenic relationships of the viruses with swine hemagglutinin (Hsw1) and human hemagglutinin H0. One of the strains had neuraminidase N2 the other two Nav2. An analysis of the polypeptide composition of the virus showed the molecular weights of the heavy (HA1) and light (HA2) hemagglutinin chains to be similar in both strains (about 50,000 and 25,000 daltons, respectively). The strains had a low content of the light hemagglutinin chain (HA2) which is typical for viruses having Hsw1 hemagglutinin.

Animal Population Groups↗
PubMedDate not supplied

[Tissue reaction and influenza virus infectiousness during multiplication in tissue culture in the presence of remantadine].

The effect of remantadine in tissue culture of primary chick embryo fibroblasts (CEF) infected with classical fowl plague virus (FRV) was found to be dual. On the one hand, in low concentrations remantadine effectively inhibited in CEF reproduction of the virus sensitive to it. On the other, in high (subtoxic) concentrations in CEF infected with either sensitive or remantadine-resistant FPV variants it induced virus reproduction. The latter became noninfectious for normal CEF but retained its capacity for multiplication in cells treated with high concentrations of remantadine.

Adamantane↗