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Biomedical subjects

V I Votiakov

Publications and source records attributed to V I Votiakov.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

[Photodynamic inhibition of infection caused by herpes simplex virus type 1 in the cultured cells, by using 5-aminolevulinic acid-induced porphyrins].

On simulating infection caused by different herpes simplex virus type 1 (HSV-1) variants responsive and unresponsive to the drugs acyclovir and phosphonoacetic acid in the cultured Vero and C6 cells has revealed the higher ability of target cells to accumulate 5-aminolevulenic acid (ALA)-induced endogenous porphyrins, which determines the selectivity of their photo damages. Optimal conditions have been defined for all the studied HSV-1 variants to show a virus-inhibiting effect upon photodynamic exposure of infected and ALA-treated cell cultures.

Aminolevulinic Acid↗
PubMedDate not supplied

[Experimental herpetic infection of the CNS with predominant involvement of the spinal cord].

Clinical, morphological, and virological characteristics of a model of herpes infection of the CNS with predominant involvement of the spinal cord, an acute ascending myelitis with primary demyelinization, are presented. The development of spinal cord lesions after HSV inoculation into the spleen was observed in different species of laboratory animals: mice, hamsters, guinea pigs, and rabbits; some animals (30%-70%) developed generalized infection with the involvement of the brain. The frequency of the spinal cord involvement was found to depend on the age of the animal and the dose of the virus-containing inoculum. Differences in the clinical and morphological manifestations of herpes infection were due to different mechanisms of herpes simplex virus spread in the body: either centripetally on the axons of the visceral nerves innervating the spleen into the thoracal cord, or hematogenic disseminations with virus penetration across the hematoencephalic barrier. The total duration of the disease was 3-12 days depending on the animal species.

Aging↗
PubMedDate not supplied

[The formation of infectious scrapie-like structures in the persistence of the agent of amyotrophic leukospongiosis in a brain cell culture].

Electron microscopic analysis of specimens from guinea-pig brain cell cultures infected with amyotrophic leucospongiosis agent (belonging to "unconventional" viruses) revealed accumulation in the culture fluid of abnormal filamentous structures similar to scrapie-associated fibrils (SAF) differing in morphology. Most of these SAF-like structures 10-15 nm in diameter contained helically wound protofilaments with a repeat at certain intervals (50-150 nm). When these structures were inoculated into guinea-pig brain astrocyte cultures they produced dystrophic-destructive changes in some (25%) astrocytes, and their intracerebral inoculation to guinea pigs produced an experimental disease. The abnormal SAF-like structures were reisolated from the brains of the inoculated animals which indicated the relationship between these structures and infectivity.

Animals↗
PubMedDate not supplied

[Cellular transformation in an MDCK culture caused by the agent of amyotrophic leukospongiosis].

The possibility of infecting epithelioid MDCK cell culture with the agent of amyotrophic leukospongiosis (AL) was shown. Various morphofunctional changes were observed in the infected cells. In the first subpassage, the saturation density and yields of cells increased, while their mitotic activity decreased and adhesive properties changed. In the second subpassage, the cell morphology and monolayer architectonics changed, the cells losing the ability to form a confluent monolayer. The infected cultures died in further subpassages. Clinical signs of the disease and morphological changes in the CNS typical of AL were observed in the animals infected with a homogenate of cells and culture fluid from the AL-agent-infected cultures.

Animals↗
PubMedDate not supplied

[Viral retrograde-like pattern of neuronal degeneration in experimental amyotrophic leukospongiosis].

The pattern of destructive alterations of neurons and their processes at different spinal cord levels in guinea pigs with experimental amyotrophic leukospongiosis (AL) was studied by light and electron microscopy. The destruction of a portion of neurons was found to be of retrograde degeneration type. Electron microscopy of spinal conduction pathways showed that degenerative alterations occurred not only in the neuronal soma or processes but also in synaptic structures. The results indicate primary involvement of nerve cell processes, particularly axons, in experimental AL. It is suggested that AL pathogenesis is pathomorphologically based on the uptake of the agent by axonal terminals.

Animals↗
PubMedDate not supplied

[Experimental infection caused by Machupo virus in intact and immunosuppressed albino mice].

Features of the course of experimental infection in white mice caused by Machupo virus in relation to their age, route of virus inoculation, and under conditions of normal and suppressed immune response created by antithymocyte globulin (ATG) were studied. The effect of different ATG doses and intervals of its administration on the experimental infection were demonstrated.

Animals↗
PubMedDate not supplied

[Change in the influenza virus upon multiplication in the presence of high concentrations of remantadine].

It was established that classical fowl plague virus Rostock (H7N1) propagated in culture to which 10-25 micrograms/ml of remantadine had been added differed from the original FPV and its remantadine-resistant variant by markedly reduced infectious and hemagglutinating activity and, to a lesser extent, neuraminidase activity, lower amounts of M protein and hemagglutinin, incomplete cleavage of hemagglutinin, and significant loss of spikes on the virion surface.

Adamantane↗
PubMedDate not supplied

[Isolation of influenza virus hemagglutinin and its separation into subunits by a stage-by-stage scheme for viral protein fractionation].

The virus envelope protein, hemagglutinin, recovered by DTAB extraction of influenza virions, was purified to a high degree by the gel filtration high-performance liquid chromatography (GF HPLC). Then hemagglutinin was reduced and alkylated with iodoacetamide and the subunits were resolved by GF HPLC on the columns Spherogel TSK G 2000 SW and 3000 SW (600 X 7.5 mm ID).

Chromatography, Gel↗
PubMedDate not supplied

[Antiviral factor isolated from infected cell cultures].

An antiviral factor of protein nature inhibiting reproduction of VEE, herpes, vaccinia VSV, fowl plague viruses was isolated from infected cell cultures. The factor has no virucidal or prophylactic effect, stable to low pH values and heating at 100 degrees C for 30 min.

Animals↗
PubMedDate not supplied

[Virus replication in a culture of smooth muscle cells from the human aorta].

A new modification of the method for cultivation of cells from blood vessel explants has been proposed, and on its basis several cell lines from different vessels of human embryo and adult subjects have been derived. The sensitivity of vessel cells to viruses was studied on a model of aorta cell culture using a wide spectrum of RNA and DNA viruses. All the strains under study with the exception of respiratory-syncytial virus (RSV) and encephalomyocarditis (EMC) virus induced a productive infection in the cells. The infection with RSV and EMC was abortive. The possibility of virus replication in vessel cells was confirmed by isolation of adenovirus from a human renal artery biopsy.

Aorta↗
PubMedDate not supplied

[Effect of organic solvents on streptokinase-initiated fibrinolysis].

Proton-developing (ethanol, I-propanol, I-butanol, I-pentanol) and aproton solvents (dioxane, dimethylsulfoxide, dimethylformamide) at concentrations 1.5-50% activated the streptokinase-initiated fibrinolysis. Methanol and acetone were not effective. The rate of fibrinolysis, studied in presence of glycerol and phenol (used at 0.1-9.3% concentrations), depended on concentrations of these reagents in samples. Efficiency of aliphatic alcohols was increased with elongation of hydrocarbonic chain. The activating effect of ethanol was not removed by epsilon-aminocapronic acid 10(-2) M.

Fibrinolysis↗