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Biomedical subjects

V K Kozlov

Publications and source records attributed to V K Kozlov.

At least 19 recordsLinked to original sources
PubMed2002

[Immunopathogenesis of severe wounds and traumas: possibilities of immune correction].

The authors describe the present-day views on the nature of immune dysfunctions in severe traumas. Based on personal clinical experiences and literature data the authors discuss the role of immune dysfunctions in pathogenesis of the traumatic disease. Special attention is given to the role of the immune system in the development of the life-threatening condition: polyorganic insufficiency whose formation mainly results from disorganization and functional failure of the system of immune reactivity. Clinical investigations have shown high effectiveness of early administration for severe wounds and traumas of a new means of immunocorrection--yeast recombinant interleukin-2 of man (preparation Roncoleukin). The administration of this immunocorrector in complex schemes of intensive therapy of the victims was shown to prevent the development of severe pyo-septic pathology and perfectly change the course of the traumatic disease.

Adult↗
PubMed1986

[Immunologic and pharmacologic activity of atropine-protein conjugates].

The immunological (production of specific antibodies to hapten) and pharmacological activity of bovine serum albumin conjugates with different chemical modifications of atropine was investigated. Mice and rabbits treated with atropine-protein conjugates (1-10 mg/kg) have produced a considerable number of specific serum antibodies to hapten during primary and secondary immune response. Mice treated with atropine-protein conjugates (25 and 50 mg/kg) have produced undetectable amounts of specific serum antibodies. The study of pharmacological activity in mice has revealed peripheral activity of atropine-protein conjugates. There was a correlation between the pharmacological activity of atropine-protein conjugates (25-50 mg/kg) and the activity of atropine (5 mg/kg), however the pharmacological effect of atropine-protein conjugates was somewhat longer. The results suggest that atropine-protein conjugates are both immunologically and pharmacologically active compounds.

Animals↗
PubMed1985

[pH changes in the matrix of serotonin organelles of stimulated thrombocytes].

The changes of pH gradient on serotonin organelle membranes can be observed using the fluorescent marker acridine orange. This gradient decreases with thrombin activation of the blood plates. The fall of delta pH can be induced by ammonium salts which also influence the ADP-induced cellular aggregation. NH4 ions added simultaneously with low doses of ADP potentiate cellular aggregation, and added after ADP-induced interaction of cells lead to another wave of intercellular contacts inhibited by mepacrin. Fluorescent acridin aminoderivatives can be used both for kinetic observations of delta pH on the serotonin organelle membranes of native platelets and for studying the interaction of the systems responsible for maintaining this gradient with the systems of prostaglandin and adenine nucleotide metabolism.

Adenosine Diphosphate↗
PubMed1985

[Characteristics of the activation of rabbit platelets induced by 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet activation factor)].

A study was made of the action of alkyl acetylglycerophosphocholine, a semi-synthetic platelet activation factor. The compound was shown to have both marked aggregation activity and to interact with serotonin granules (5-HT-organelles) of the cells, inducing the release of the fluorescent marker acridine orange. Some features of the platelet aggregation factor interaction with platelet rich plasma suggest that the latter contains enzymes inducing degradation of the factor.

Animals↗
PubMed1981

[pH gradient in the membranes of serotonin-containing granules of thrombocytes: principles for studying them using fluorescent amino-derivatives of acridine].

A new method has been devised for determination of gradient pH and buffering capacity in 5-HT-containing granules of platelets. It is based on the ability of 5-HT granules to accumulate acridine derivatives so that the ratio of the label concentrations outside and inside is equal to the proton concentration ratio. Simultaneous recording of the fluorescence intensity and light transmission of the platelet-rich plasma has been made use of in comparing the kinetics of the intragranular pH collapse and platelet aggregation.

Acridine Orange↗
PubMed1980

[Electron-acceptor (acid) properties of the contents of blood platelet intracellular granules and their significance for the reactions of these cells].

The fluorescent marker method and measurements of aggregation were utilized to study the significance of the acidic intragranular space in blood platelets. Acridine orange is liberated from 5-HT granules after alkalinization by the ammonia ion which inhibits the induction of platelet aggregation by ADP and causes the platelet disaggregation. At low doses, ammonia ion enhances ADP-induced aggregation. The transformation kinetics of 5-HT granules was investigated in the course of clot formation with a new instrument that simultaneously monitors the light transmittance (aggregation, clot formation) and fluorescence intensity (accumulation and liberation of fluorescent marker). These experiments provide the evidence that processes of fibrin filament sedimentation and fibrin-blood platelet interaction were followed by a liberation of fluorescent marker from 5-HT granules.

Animals↗
PubMed1979

[Lysosomes-like thrombocyte alpha-granules: the activation and inhibition of the release of the contents in blood coagulation].

The mechanism of the release of alpha-granules constituents of thrombocytes was studied in the course of blood coagulation utilizing the fluorometric technique. The release reaction can be induced by proteolytic enzymes. The process is stimulated by the presence of extracellular Ca2+. This pattern of release reaction belongs to "release II" type. Aminazin can also induced release of alpha-granules constituents. Heparin inhibited release II induced by Ca2+ in citrated plasma, whereas streptase had no effect.

Animals↗
PubMed1979

[Reversible endocytosis apparatus of the blood platelets].

The functioning of ganules of blood platelets participating in the reversible endocytosis reaction was investigated in rabbits. Eczocytosis was shown to be initiated by inducers of two types: proteolytic enzymes and compounds accumulated by the granules (biogenic amines, dyes, anesthetics and phenothiazines). Heparin inhibits thrombin-induced eczocytosis. During eczocytosis produced by accumulation of compounds by blood platelet granules, the cells lose their capacity for maintaining the aggregation state. The nature of blood platelet granules participating in the reversible endocytosis reaction is discussed.

Animals↗