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Biomedical subjects

V M Orlov

Publications and source records attributed to V M Orlov.

At least 19 recordsLinked to original sources

[Complete primary structure of Bacillus thuringiensis extracellular ribonuclease].

Complete primary structure of an extracellular low molecular mass ribonuclease of Bacillus thuringiensis was determined using Edman degradation and mass-spectrometry analysis of individual peptides obtained after hydrolysis of the protein by cyanogen bromide and staphylococcal protease. The peptides were isolated and purified by HPLC and denaturing PAGE. The enzyme consists of 109 amino acid residues (Asp 8, Asn 6, Thr 6, Ser 10, Glu 3, Gln 1, Pro 3, Gly 9, Ala 12, Val 7, Ile 7, Leu 7, Tyr 7, Phe 4, His 1, Arg 10, Trp 3 and Lys 5) and has a molecular weight of 12182 Da. A single difference was detected between primary structures of the enzyme and an extracellular ribonuclease of B. intermedius.

Amino Acid Sequence↗

[Clinical use of echotomography with target aspiration biopsy and urgent cytological examination].

Upon analysis of the results obtained at cytological examinations of the biopsies performed by target aspiration puncture under the control of ultrasound tomography in 240 patients with various diseases, it was established that combination of aspiration puncture, ultrasound tomography and rapid cytological evaluation improve diagnosis of various diseases, reduce the duration of the examination, increase the quality of treatment.

Biopsy, Needle↗

Location of placenta and clinical course of labour.

The results of studies on 2396 women are presented. Relations were found between location of placenta and 'ripeness' of uterine cervix, duration of labour, prognostic value of intrauterine pressure, complications of the third stage, fate of the newborn and success of induction of labour. The authors arrived at the conclusion that attachment of the placenta to the fundus uteri may be considered an unfavourable location causing an increase in the number of different deviations from normal labour.

Apgar Score↗

[Study of energy characteristics of 5-fluorouracil analogs by photoionization mass-spectrometry].

The fragmentation pathways of N-1-alkoxyalkyl derivatives of 5-fluorouracil and various analogues of an antitumor drug ftorafur have been examined using a mass-spectrometry technique. The ionization and appearance energies for major ions of the compounds under study have been determined on the basis of the ionization efficiency curves obtained using photoionization mass-spectrometry. Different transport forms of 5-fluorouracil have been demonstrated to be similar in the stability of their pseudoglycosidic C-N bond.

Chemical Phenomena↗