Quantitative ultramicroanalysis of amino acids in the form of their DNS-derivatives. 3. Analysis of natural amino acids. Characteristics of the method.
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Biomedical subjects
Publications and source records attributed to V M Orlov.
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Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.
Combined investigation of 200 patients with different thyroid lesions was conducted. The program of investigation included computerized echotomography of the thyroid with spot puncture and rapid cytological examination. Of them 138 patients were operated upon. In 125 patients (87.4%) combined investigation including spot aspiration puncture biopsy and echotomography helped to establish morphological diagnosis of lesion, to specify indications for surgical intervention and to optimize its use.
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By photoionization mass spectrometry the energy characteristics were investigated for a series of nucleosides whose triphosphates possess anti-HIV activity. The electron-donor ability (a value reciprocal to ionization energy) does not correlate with the biological activity of corresponding nucleotides. The energy of glycoside bond rupture depended on modification in deoxyribose.
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