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V N Mineev

Publications and source records attributed to V N Mineev.

At least 19 recordsLinked to original sources
PubMed2004

[Surface architectonics and cytoskeleton of erythrocytes and their modulation by adrenergic agents in patients with bronchial asthma].

AIM: To evaluate erythrocyte surface architectonics and cytoskeleton in bronchial asthma (BA) under modulation of adrenergic agents. MATERIAL AND METHODS: 24 healthy persons, 61 patients with bronchial asthma (BA) were examined. Of them, 28 patients had allergic BA (ABA) and 33 ones had nonallergic BA (NABA). Erythrocyte surface architectonics was studied by phase-contrast microscopy. Fixation in 0.5% solution of glutar aldehyde was used, preparation "squashed drop" was prepared. In all preparations adrenalin hydrochloride and obzidan were used in equimolar final concentrations of 4.10(-5) M. The mean morphologic index of transformation that reflects the shift to stomatocytosis or to echinocytosis was estimated to characterize integrally erythrocyte surface architectonics. The cytoskeleton was studied by the modified method of Chentosov's et al. The integral optic density was evaluated on imaging analyzer of Ista-Videotest company (St-Petersburg). RESULTS: In healthy persons there was a correlation between erythrocyte surface architectonics and cytoskeleton under modulation of adrenergic system. Adrenalin-induced decrease of cytoskeletal proteins correlated with the shift to stomatocytosis. In NABA erythrocyte surface architectonics was characterized by a pronounced shift to stomatocytosis. This shift was accompanied with the most pronounced decrease of cytoskeletal proteins and it reduced in remission. In ABA manifestation of the shift was minimal and did not depend on the phase of the disease. In ABA no changes in erythrocyte surface architectonics in the presence of adrenalin were revealed. In the same conditions a decrease in cytoskeletal proteins was found. CONCLUSION: A correlation was found between erythrocyte surface architectonics and cytoskeleton in healthy persons. In ABA, under modulation of adrenergic system by adrenalin dissociation was revealed between receptor and cytoskeletal mechanisms of cell form induction. This dissociation does not depend on the phase of the disease and is thought to be one of the important postreceptor disorders typical for this variant of the disease.

Adrenergic beta-Antagonists↗
PubMed2004

[Bronchoobstructive syndrome in bronchoectasias].

AIM: To specify mechanisms of bronchial obstruction in bronchoectasia (BE). MATERIAL AND METHODS: Clinical, x-ray, bronchological and spirographic examinations with broncholytic test were made in 26 BE patients aged 16-66 years (14 males, 12 females). The membrane-receptor complex of erythrocytes was based on the effect of adrenoantagonist propranolol on osmotic erythrocytic resistance (obsidan test) and effect of adrenoagonist adrenalin on dynamics of ESR. RESULTS: Allergological anamnesis was detected in 69% of BE patients, hereditary allergological anamnesis--in 22%, membrane-receptor changes characteristic for atopic condition by obsidan test--in 46%, fractional erythrocytic sedimentation with adrenalin--in 42%. Bronchoobstruction syndrome occurred in 37% cases with mean values FEV1 46 +/- 6.2%, MSV50 17.4 +/- 3%, MSV75 14.4 +/- 0.9%. The broncholytic test detected a mean increment of FEV1 by 12.2 +/- 3.1%. Latent bronchospasm was revealed in 33% patients with BE. CONCLUSION: The correlation analysis suggests that among mechanisms of bronchial obstruction in bronchoectasia, bronchospasm is of great importance especially in allergological anamnesis pathogenesis of which involves disorders of membrane-receptor complex.

Adolescent↗
PubMed2004

[Phenomenon of hyperexpression of ribosomic cistrons in bone marrow hemopoietic elements in bronchial asthma].

AIM: To study expression of ribosomic cistrons (RC) of bone marrow hemopoietic elements (BMHE) in bronchial asthma (BA). MATERIAL AND METHODS: A total of 37 bronchial asthma (BA) patients were examined. Of them, 20 ones had allergic BA (ABA) and 10--nonallergic BA (NABA). Seven patients received systemic glucocorticoid hormones (SGH). Control included cells of bone marrow from 7 healthy donors. Transcription and maturation of pre-rRNA in BMHE were analysed using bone marrow samples obtained during the sternal puncture (a modified method of Howell and Black). RESULTS: RC activity was high. It depended on BA variant and the disease phase. In ABA high RC activity persisted in remission. GCH suppress RC activity rise. The maximal effect of GCH suppressive action was seen in myeloid lineage, minimal--in erythroid one. The effect is absent in more mature erythroid cells and bone marrow lymphocytes. CONCLUSION: The phenomenon of RC activity rise in myeloid, erythroid and lymphoid lineages of the bone marrow of BA patients may reflect contribution of the bone marrow to formation of BA pathogenesis.

Asthma↗
PubMed2003

[Effect of adrenergic and histaminergic disorders on the subclinical course of atopic bronchial asthma].

To characterize membrane-receptor peculiarities of the adrenergic and histaminergic systems under the model of peroxide and hyperosmolar effect in atopic bronchial asthma (ABA) and preclinical stage of BA, we have examined 25 patients with ABA, 19 patients with nonpulmonary allergy, 28 healthy men and 21 healthy blood relatives of ABA patients. The phenomenon of inversion of the effect of beta-adrenoblocker (obzidan) and of the combined effect of histamine and H1-antagonist (dimedrol) was revealed in ABA patients. The same phenomenon was registered at the preclinical stage of BA. In ABA and in preclinical BA the shift of H1/H2-histaminergic balance to increased H1-activity exists. Peroxide and hyperosmolar effects modeled on red cell membranes allowed us to characterize reactivity of adrenergic and histaminergic systems not only in ABA but also in preclinical BA.

Adrenergic beta-Antagonists↗
PubMed2002

[Erythrocyte insulin-reactive system and carbohydrate metabolism in bronchial asthma].

AIM: Analysis of insulin-reactive system of erythrocytes and carbohydrate metabolism in bronchial asthma (BA) at cell and other levels. MATERIAL AND METHODS: 106 patients with BA, 26 healthy subjects and 10 healthy subjects with familial history of atopy participated in the study. Glucose tolerance and colorimetric tests were made to measure glucose, glycosylated hemoglobin, respectively. Erythrocytic insulin reactivity was assessed using an original method. Bronchial resistance was determined and specific bronchial conduction was calculated. Acetylcholine test was conducted before and after a single inhalation of 5% isoosmolar glucose solution. RESULTS: Atopic BA was characterized by a trend to hypoglycemia, low glycosylation of red cell hemoglobin, impaired membrane transport of glucose in red cells, a hypoglycemic type of glucose tolerance test curve, a paradoxical reaction of erythrocytes to insulin and adrenaline--release of glucose. CONCLUSION: The detected changes in cell insulin reactivity in atopic BA and positive effects of 5% glucose solution inhalations on bronchial hyperreactivity in BA patients provide grounds for development of new therapeutic approaches in BA.

Adolescent↗
PubMed2000

[Membrane-receptor disorders in adrenergic and histaminergic systems under peroxide exposure in bronchial asthma patients].

AIM: To characterize membrane-receptor peculiarities in adrenergic and histaminergic systems under model peroxide effect in bronchial asthma (BA). MATERIALS AND METHODS: 172 patients with BA, 17 patients with nonpulmonary allergy, 38 healthy men and 19 healthy blood relatives of BA patients were examined for peroxide resistance of erythrocytic membrane to modulation with adrenergic and histaminergic agonists and antagonists. The modified method by A. A. Abramov and A. A. Pokrovsky was used. RESULTS: In atopic BA the phenomenon of inversion of the effect of beta-adrenoblocker (obzidan) and of combined effect of histamine and H1-antagonist (dimedrol) was revealed. The same phenomenon was registered at the stage of preasthma and at the preclinical stage of BA. In infection-dependent BA this phenomenon occurred less frequently being rare in remission. In both BA variants the shift of H1/H2-histaminergic balance to increased H1-activity exists which is more significant in atopic BA, preasthma and preclinical BA. CONCLUSION: Peroxide effects modelled on red cell membranes enabled to characterize reactivity of adrenergic and histaminergic systems not only in BA but also in preasthma and preclinical BA.

Adrenergic Agonists↗
PubMed2000

[Role of biological defects in formation and development of bronchial asthma and potentialities of premorbid diagnosis].

The paper considers bronchial asthma as a polygenic disease. It presents some data available in the literature on the role of genetic disorders in the development of atopic states and the authors' own findings of various biological defects detected in apparently healthy blood relatives of patients with bronchial asthma. It is concluded that premorbid diagnosis is of importance in high risk persons.

Asthma↗
PubMed1999

[Membrane receptor disorders in bronchial asthma].

AIM: To give impedance characteristics of erythrocytes in bronchial asthma (BA) and to determine the relation between the impedance parameters and specific features of the membrane-receptor complex reflecting the condition of cell membrane lipid matrix. MATERIALS AND METHODS: 164 patients with BA. 19 patients with preasthma, 21 normal blood relatives of BA patients and 36 healthy controls were examined. Parameters of electrical impedance (capacity and temperature-dependent) were measured by modified method of S. Takashima et al. (1988). Peculiarities of low-temperature hyperosmolarity hemolysis were studied before and after modulation with adrenergic and lipotropic agents. RESULTS: Atopic BA was characterized by increased erythrocyte electrical capacity and inversion of adrenaline and obzidan effects on electrical impedance, initially high erythrocyte resistance to low-temperature hyperosmolarity effects. Bacterial asthma demonstrated the opposite trend in both impedance and hemolysis parameters. CONCLUSION: There is low erythrocyte membrane microviscosity in atopic BA, but high microviscosity in bacterial asthma. Basing on these findings the authors propose to use essential phospholipids in the treatment of bacterial BA.

Adult↗
PubMed1991

[Membranoreceptor disorders in the pathogenesis of bronchial asthma].

The purpose of the work was to study some characteristic features of the membranoreceptor complex in patients suffering from atopic bronchial asthma (BA) and infection-dependent BA. That was done with the aid of the erythrocytic model (phytohemagglutinin /PHA/ reception, electrophysical analysis of the membranous properties). Electrophoretic mobility (EPM) of red blood cells and red blood cell sedimentation under the action of PHA were determined. The data obtained indicate that in patients with infection-dependent BA, the membranoreceptor complex is characterized by a decrease of EPM of red blood cells, inhibition of red blood cell sedimentation in the presence of PHA. The results of studying the membranoreceptor complex in BA patients are summarized as are the marker characteristics of atopic and infection-dependent BA, reflecting pathology of reception, glycocalyx, and lipid matrix.

Asthma↗
PubMed1991

[Acupuncture therapy in the treatment of patients with bronchial asthma].

Erythron membrane impairment was investigated using membrane -active drugs (obsidan, delagil, morphium, ethanol) to specify indications to acupuncture in bronchial asthma (BA). The patients were studied during the treatment and followed up for a year. It was found that both immediate and long-term results of acupuncture were related to patterns of the membrane impairment. BA patients with a sharp, greater than 1.5 fold diminution of microcyte count upon delagil test should be assigned to repeated courses of acupuncture during a year. To monitor the treatment effect, it is recommended to define osmotic resistance of the red blood cells in obsidan test in addition to clinicofunctional indices.

Acupuncture Therapy↗
PubMed1990

[The potentials of a unified polyclinic-hospital system in raising the quality of the diagnosis and the efficacy of the differentiated therapy of bronchial asthma patients].

The authors review potential approaches to the upgrading of the diagnosis at the out- and inpatient stages of the rendering medical assistance to patients suffering from bronchial asthma (BA). Show the necessity of the counselling specialized aid, additional studies of the signs of atopy, wise combination of non- and medicamentous treatment methods to enhance the efficacy of the treatment of BA patients. Provide evidence for the advisability of solving the organizational problems concerned with a closer interaction of the polyclinic and hospital with the leading medical institutions on the problem "Pathology of the Respiratory Organs".

Adolescent↗
PubMed1989

[Atopic bronchial asthma as pathology of the membrane-receptor complex].

The pathogenesis of atopic bronchial asthma (ABA) is examined from a membranologic viewpoint, with a methodological approach, postulating membrane-receptor pathology as the key pathogenetic element of the disease, taken as the basis. Major results of the study of the adrenoreactive system are analysed, and pathogenetic significance of the demonstrated paradoxal cell reaction to beta-adreno-antagonist is pointed out. Prospects related to the development of studies of morphofunctional erythrone properties as a new line of research in BA pathogenesis are demonstrated, and erythrocyte qualities applicable as marker of atopia, such as microcytosis and membrane glycoprotein distribution, are identified. Specific pattern of cell membrane response to aldehyde compounds seen in ABA is described. The data obtained are a development of the earlier-postulated hypothesis of ABA-associated dedifferentiation. A notion of ABA as a membrane-receptor disease is proposed.

Asthma↗
PubMed1989

[Features of absorption of cortisol by peripheral blood cells in patients with bronchial asthma].

Hydrocortisone absorption by lymphocytes and red blood cells was reduced in patients with bronchial asthma (BA). Adrenaline produced the stimulatory effect on hydrocortisone absorption by lymphocytes and red blood cells in practically normal subjects and in BA patients in whom that effect was decreased. In patients with the steroid-dependent clinico-pathogenetic pattern of BA, hydrocortisone absorption by lymphocytes was reduced to a considerable degree. It is stressed that cellular mechanisms of glucocorticoid insufficiency are implicated in the BA pathogenesis.

Asthma↗
PubMed1989

[Features of erythrocyte aggregation in various forms of bronchial asthma].

A model of aggregation of erythrocytes was used to study the characteristic features of membrane disorders in different forms of bronchial asthma. It was found that erythrocytic aggregations depended on the form of bronchial asthma, primarily due to the characteristic features of the erythrocytic cellular membrane. In infectious-dependent bronchial asthma and chronic obstructive bronchitis erythrocytic aggregation was increased while in atopic bronchitis it tended to decrease.

Asthma↗
PubMed1988

[Level of specific binding of beta-adrenergic ligands and the morphofunctional properties of erythrocytes in bronchial asthma].

Peripheral blood erythrocytes of patients with bronchial asthma (BA) and preasthma (PA) were investigated using the following methods: 1) the radiochemical method--the level of specific binding of beta-adrenergic ligands; 2) direct erythrocytometry--erythrocyte diameter; 3) the cytochemical method (paraldehyde fuchsine/PAF/-reaction)--membranous glycoproteins. The level of erythrocytic specific binding of beta-adrenergic ligands was decreased in BA and PA patients. The level of small size erythrocytes (SSE) was on an increase in patients with atopic BA, extrapulmonary allergoses as well as in healthy persons with aggravated atopic heredity. The content of PAF-negative erythrocytes turned out to be considerably raised in atopic BA. A conclusion was made of general disorders of the membranoreceptor system as a fundamental cause of allergically changed reactivity.

Adolescent↗