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Biomedical subjects

V P Kuznetsov

Publications and source records attributed to V P Kuznetsov.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

[Expression of receptors for human alpha- and gamma-interferons on the surface of peripheral blood mononuclear cells in viral infections].

Expression of receptors on peripheral blood mononuclears of donors and patients with viral infections during therapy was studied using FITC-labeled monoclonal antiidiotypical antibodies with the "internal image" of human alpha- and gamma-interferons and monoclonal antibodies to these interferons. Activation of the immune system caused by infection modifies the expression of interferon receptors and leads to appearance of membrane-bound interferons, mainly gamma-interferon.

Animals↗
PubMedDate not supplied

[Efficiency of immunocorrection using cytokines in the therapy of papillomavirus infection].

The data of the immunological examination and the results of treatment of 86 patients with papillomavirus infection (PVI) are presented. The multifactor suppression of cell-mediated and humoral immunity was established. The degree of immune disturbances correlated with the spread and severity of lesions. The use of systemic injections of cytokines (leukinferon and concentrated interferon) in low doses as adjuvant therapy with laser-radiosurgery led to the normalization of most immunological characteristics and the course of PVI without relapses.

Adjuvants, Immunologic↗
PubMedDate not supplied

[Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid].

Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Physico-chemical, immunochemical and biological properties of the amnionic prolactin were studied as compared with those of hypophyseal hormone. Properties of the hormonal forms isolated from both these tissues were similar. The glycosylated form of prolactin with molecular mass 25.0 kDa was detected either in amnionic fluid or in hypophysis.

Amino Acids↗
PubMedDate not supplied

[Antiradiation effects of leucynferon on dogs and guinea pigs].

In experiments with two species of animals (dogs, guinea pigs) irradiated with sublethal and lethal doses of gamma-rays, it was observed, that leucynferon had antiradiation effect. Course of injections: dogs--8 injections subcutaneus: 2.0 ml (1, 3, 5, 8, 12, 14, 21, 34 days after irradiation); guinea pigs--14 injections subcutaneus, 0.2 ml (1-14 days after irradiation). Therapeutical effect was explained by capacity of the preparation to defend the hemopoietic organs from the radiation and to stimulate hemopoiesis. Leucynferon hindered the development of acute radiation sickness symptoms. Immunoreactivity of dogs and guinea pigs in experimental group was more complete and restored faster. The growth of the automicroflora on the skin was restrained. Production of interferon-gamma (which is a function of T-lymphocytes) was restored faster.

Animals↗
PubMedDate not supplied

[Tumor necrosis factor in medicinal interferon preparations].

In response to virus induction a culture of donor leukocytes alongside with interferon (IF-alpha) produced a factor of tumor necrosis (TNF). The kinetics of TNF and IF-alpha biosynthesis did not depend on the kind of IF used for priming, was rapid, with maximum production within 7-8 hours. Antibodies to IF-alpha and IF-alpha had no effect on TNF production, while antibody to TNF did not reduce IF-alpha yields. TNF in detectable titres was present in medical preparations of native IF-alpha but was absent in preparations of recombinant IF-alpha and IF-alpha as well as in an injection preparation of IF purified by chemical methods.

Antibodies↗
PubMedDate not supplied

[The humoral immunity system of mice with experimental slow influenzal infection].

The status of the interferon system and level of immunoglobulins were studied in C57BL6 mice with slow influenza infection. These mice showed signs of immunosuppression: low endogenous interferon production, synthesis of alpha- and gamma-interferon by splenocytes of these mice in vitro 4-8 times lower than by those of the controls, lower levels of IgG in the blood serum. These data indicate general suppression of humoral immunity.

Aging↗
PubMedDate not supplied

[Effectiveness of human leukocytic interferon in children with papillomatosis of the larynx, trachea and bronchi].

The results of interferon therapy of 32 children with severe forms of laryngeal, tracheal, and bronchial papillomatosis are very encouraging. Clinical recovery (no relapses during 3 years or longer) was observed in 8 patients, improvement (no relapses during less than 3 years or 2-3-fold prolongation of no-relapse periods) was seen in 12 patients, no changes were recorded in 10 children; data on 2 other patients were too limited or lacking at all. The efficacy of interferon therapy of airways papillomatosis can be further increased, if doses are selected on an individual basis.

Adolescent↗