Biomedical subjects
Publications and source records attributed to V Plaisner.
The cell transformational test, consisting in morphological transformation of the human embryonal lung cells, is a rapid and sensitive method of identification of chemical carcinogens. The test is capable of revealing carcinogens undetectable by other methods and it makes it possible to avoid mistakes due to inter-species differences in the biotransformation of the drugs tested.
This paper reports an investigation of the biological effects of monomers of N-substituted (meth)-acrylamides used in the preparation of polymers suitable for medical purposes. By combining tests carried out on cell cultures and on living animals, we found thpheral nervous system. Tests performed on cell cultures can be used as preliminary and supplementary ones in testing the toxicity of substances on living animals.
The CV-1 cell line, derived from Cercopithecus aethiops monkey kidneys, acquired in the course of serial passaging an epitheloid morphology and heteroploid karyotype. As compared with Vero and GMK cell lines, the CV-1 line proved to be more susceptible to a broad spectrum of arboviruses belonging to the families Togaviridae and Bunyaviridae. Most of them reached high titres and were markedly cytopathic in CV-1 cells so that all tests based on the cytopathic effect could be carried out. The CV-1 cell line can thus be recommended as an extra-ordinarily suitable substrate for the diagnosis and study of arboviruses.
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The healing-in of implants of terpolymer gels having different chemical composition was studied in animals. Histological analysis showed that all polymers under investigation are healed-in in a similar way by encapsulation with collagen fibrous tissue. Neither cancerogenic effect nor calcification were observed within the interval studied.
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The determining factor in vaccinia virus reproduction is the initial pattern and subsequent development of the given cell populations, in relation to the infection multiplicity, interferon production and other factors. The sensitive cells in fresh suspensions may be the blood monocytes and the small lymphocytes are virus-resistant. In cultures, transformed lymphocytes (blast cells) and macrophages play the major role in virus reproduction. The macrophages are probably of greater significance for the production of infectious virus. Replication of virus antigen was initiated in the polymorphonuclear cells in exceptional cases only, and only in the presence of a larger infection dose.
Autoclavable culture media containing an increased (10---15-fold)concentration of succinate buffer permit a comparitively long-term cultivation of cells in free gas exchange with the atmosphere. Based on them, an economical technique of plaque titration of arboviruses on plastic panels with methylcellulose overlay was developed. With seven arboviruses of three different groups and three cell lines (CV-1, PS and PK), the method proved sufficiently sensitive as compared with titrations in mice or tube cell cultures and suitable for plaque reduction tests.
Of several cell lines tested, the CV-1 monkey cell line proved to be the most suitable system for propagation of Crimean hemorrhagic fever (CHF) virus. Two CHF strains could be adapted to this cell line by serial passaging. The viral progeny from these passages was specifically cytocidal and produced plaques in CV-1 cells, which could be used in serological diagnosis of CHF. After adaptation of CHF virus to CV-1 cells, the rate of virus synthesis was more rapid, but the yield of virus produced by these cells did not substantially increase. Data reported on cultivation of CHF-Congo viruses are discussed in comparison with the present findings.
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