A modified plaque method for arboviruses on plastic panels.

V Hronovský; R Benda; V Plaisner

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A modified plaque method for arboviruses on plastic panels.

V Hronovský·R Benda·V Plaisner

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Abstract

Autoclavable culture media containing an increased (10---15-fold)concentration of succinate buffer permit a comparitively long-term cultivation of cells in free gas exchange with the atmosphere. Based on them, an economical technique of plaque titration of arboviruses on plastic panels with methylcellulose overlay was developed. With seven arboviruses of three different groups and three cell lines (CV-1, PS and PK), the method proved sufficiently sensitive as compared with titrations in mice or tube cell cultures and suitable for plaque reduction tests.

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Animals Antibodies, Viral Antibody Formation Antigens, Viral Arboviruses Cell Line Hemolytic Plaque Technique Mice Neutralization Tests Virus Cultivation Journal Article

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V Hronovský, R Benda, V Plaisner. 1975. A modified plaque method for arboviruses on plastic panels.. https://pubmed.ncbi.nlm.nih.gov/239561/

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