[Laser surgery and analysis of its uses in various specialties].
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Biomedical subjects
Publications and source records attributed to V Russo.
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A microplate method of enzyme-linked immunosorbent assay (ELISA) was adapted for the demonstration of antibodies to influenza A and B in 25 paired acute and convalescent sera taken from patients with influenza A infection, in 11 single convalescent sera collected from patients with acute respiratory infection and serological evidence of influenza B infection, in 14 paired sera collected from volunteers who were vaccinated with an aqueous inactivated influenza A and B vaccine, and in 15 serum samples from normal subjects. Comparison showed that ELISA was more sensitive than the hemagglutination-inhibition test. The enzyme-immunoassay gave equally reliable and reproducible results and requires very small amounts of antigen and sera. This simple test may be suitable for use in large seroepidemiological surveys.
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The Authors tested the virulicidal effect of emetine hydrochloride (EH) observed by Fusillo in clinical practice. By adding EH to HE p-2 cellular system, in a concentration such as to cause some inhibition of cellular growth, there is no meaningfull virostatic or virulicidal action against poliovirus, Mahoney's strain. EH, in the same proportion as that employed by Fusillo in human therapy, damages in the albino mouse liver. By halving the concentration of EH such a damage is not evident but no beneficial effect on the pattern of the subacute hepatitis, caused by 5 LD50 of MHV3 ensues.
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