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Biomedical subjects

V Sebestík

Publications and source records attributed to V Sebestík.

At least 19 recordsLinked to original sources
PubMed1990

Examination of serum ferritin and erythrocyte ferritin--its role in the blood transfusion service.

Blood donors were examined for serum ferritin values and concentration of ferritin in the erythrocytes. The group of male and female donors without previous donations showed average values of 102.27 ng and 51.75 ng of ferritin per one ml of serum, respectively. Males with over 20 donations had 68.04 ng ferritin per one ml, females 37.14 ng of ferritin per one ml. The reduced serum ferritin values in multiple male and female donors is statistically significant. Serum ferritin values in women of the two groups are lower than those of males, the difference also being statistically significant. In male and female blood donors, irrespective of the number of donations, average values of 13.74 ag and 12.07 ag of ferritin per erythrocyte, respectively, were established. The difference in ferritin concentration in the erythrocytes between males and females is statistically insignificant. The correlation coefficient failed to demonstrate any dependence between erythrocyte ferritin concentration and concentration of ferritin in the serum. The object of serum ferritin determination in blood donors is to detect the earliest stage of storage iron deficiency in the organism. For the latter purpose, the determination of erythrocyte ferritin is ineffective.

Blood Donors↗
PubMed1990

Serum ferritin in patients with various haemolytic disorders.

174 serum ferritin assays in 121 patients with various haemolytic disorders have been performed. The mean serum ferritin levels were significantly increased in all these disorders in contrast to healthy controls. The highest serum ferritin levels were found in pyruvate kinase (PK) deficiency, moderate increase was observed in hereditary sphaerocytosis (HS) and in autoimmune haemolytic anaemia (AIHA) with massive haemolysis and in glucose-6-phosphate dehydrogenase (G-6-PD) deficiency. Mild elevation of serum ferritin levels was depicted in paroxysmal nocturnal haemoglobinuria (PNH), in beta thalassaemia minor and in other types of haemoglobinopathies. The range of values was associated with a degree of haemolysis and its relation to duration of the disease was not apparent in most cases. Highly significant differences between serum ferritin levels in splenectomized and non-splenectomized patients with HS and between serum ferritin levels in patients with AIHA with massive haemolysis or in remission were found. As compared to normal controls, significant increase of serum ferritin levels was observed even in patients with AIHA in remission or in splenectomized patients with HS. In two patients with PK deficiency the levels exceeding 2,000 micrograms/l indicated manifest iron overload. A reliability of serum ferritin assay as an index of iron stores in haemolytic disorders has been discussed.

Adult↗
PubMed1989

Ferritin content in human thrombocytes.

Radioimmunoassay Ferritin RIA Amersham designed for the determination of splenic ferritin was used to examine the concentration of ferritin in the thrombocytes of normal subjects. The amount of 10(8) thrombocytes contained 0.62 ng of ferritin on the average.

Blood Platelets↗
PubMed1986

Serum ferritin estimation in blood donors subject to apheresis on IBM 2997 cell separator.

The serum ferritin concentration was tested in blood donors selected for apheresis on IBM Cell Separator. Of 41 donors, 28 were males and 13 females. All donors exhibited normal hemoglobin and RBC. The mean value of serum ferritin was 90.93 ng/ml in males and 48.38 ng/ml in females. In males--often with repeated blood donations--a low value of serum ferritin suggesting reduced Fe stores in organism was found in one individual only. In contrast, reduced serum ferritin levels were observed in 4 females who, before apheresis, regularly donated blood or had several pregnancies in their anamnesis. The obtained results point to prelatent or latent sideropenia. Serum ferritin concentration was measured in 18 donors selected for apheresis. The examination of ferritin was performed prior to, immediately after, and one week post separation. No significant changes in serum ferritin concentrations due to the separation procedure were observed. Preventive tests of serum ferritin in multiple blood donations and in women with previous pregnancies are recommended.

Blood Component Removal↗
PubMed1984

A study of experimental hypersplenism.

Methyl cellulose syndrome induced by repeated i. p. injections of methyl cellulose (MC) was followed in experimental rats. Following application of 2--4 MC injections early haematological changes are observed including increased values of reticulocytes, development of serum aggregation factor having anti-erythrocyte antibody nature and altered filtrability of non-washed erythrocytes. Later alterations developing with further MC injections (8-32) are characterized by expressive splenic enlargement, by decrease in erythrocyte and platelet values and by additional increase in number of reticulocytes. The cause of anaemia is pooling and sequestration of erythrocytes in the spleen and haemodilution from hypervolaemia blood plasma. The decreased platelet amount is the result of reduced survival time of platelets due to their increased sequestration in the spleen. Haematological changes are normalized after splenectomy. This picture resembles to a great extent human hypersplenism.

Animals↗
PubMed1983

Experimental elimination of the splenic function by ethyl and methyl palmitate and significance of these substances from an immunological point of view.

Our experiments with rats dealt with finding a possible functional splenic elimination by using an i.v. injection of ethyl and methyl palmitate emulsion. Simultaneously, immunological effects were determined in both substances. After applying ethyl palmitate an extensive splenic necrosis could be induced resulting in an elimination of the sequestrational splenic function to an extent comparable to surgical splenectomy. Histological examinations of liver, lungs, kidneys, heart, bone marrow, lymph nodes and thymus together with the follow-up of some metabolic indicators in the spleen and liver (determination of proteins, DNA, RNA, EFA, transaminases in blood serum and observations of proteosynthesis) showed that the effect of ethyl palmitate on the spleen is selective and that there is no marked damage of other organs. Methyl palmitate is important for its inhibition of antibody response manifesting itself in a reduced production of heteroagglutinins and in a prolonged survival time of skin semiallografts.

Animals↗
PubMed1982

The sequestration capacity of "hypersplenic" rat spleens for heat-damaged erythrocytes.

The sequestration capacity of the spleens of controls and hypersplenic rats was examined. Hypersplenism was induced by long-term intraperitoneal application of methyl cellulose. The animals were injected single doses of various amounts of heat-damaged 51Cr-labelled erythrocytes (0.1 ml to 1.5 ml); radioactivity in spleen was determined 4 hrs. following application. The amount of red cells sequestrated in the spleens of hypersplenic animals was significantly increased against the controls, after administration of massive volumes of cells. The maximum amount of erythrocytes sequestrated in the spleens of the control rats amounted to an average weight of spleens 1.25 g to 0.158 ml (0.126 ml per g of spleen), and in hypersplenic animals to an average weight of the spleen of 4.87 g to 0.283 ml (0.058 ml/l g of spleen weight).

Animals↗
PubMed1980

Experimental elimination of the splenic function by an intravenous injection of ethyl palmitate emulsion in Wistar rats.

Attempts were made at the experimental elimination of the sequestration function of the spleen on Wistar rats using ethyl palmitate (EP). Following an i.v. injection of EP emulsion in an amount of 0.35 g and 0.10 g/100 g of body weight the clearance of 51Cr-labeled and heat-damaged red cells from the blood and their sequestration in the spleen and liver at 24-h, 3-, 10-, 20-, and 50-day intervals was examined. A high dose of EP caused, notably at 24h and also after 3- and 10-day intervals, a significant decrease of radioactivity in the spleen and considerably prolonged the clearance time of the red cells. The extent of changes were comparable to those of surgical splenectomy. At later intervals (20 and 50 days after EP injection) some animals showed partial regeneration of the sequestration ability of the spleen; in some other animals the splenic damage was permanent. Changes induced by small doses of EP were less pronounced and of transient character.

Animals↗