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Biomedical subjects

V Sebestík

Publications and source records attributed to V Sebestík.

At least 37 records · Page 2Linked to original sources
PubMed1980

Sequestration of heat damaged rat erythrocytes during experimental action of methyl palmitate on RES.

The effect of an i.v. injection of methyl palmitate emulsion (MP) on the clearance of heat damaged erythrocytes from the blood and their sequestration in organs of rats was followed. Twenty-four hours following application of MP in the dose of 1.5 g/kg of body weight the survival half time of erythrocytes (T1/2(51)Cr) significantly increased, whereas the amount of sequestrated red cells in the spleen decreased. The subsequent intervals of examination, however, showed no differences as compared to the controls.

Animals↗
PubMed1979

The effect of intravenous injection of ethyl palmitate emulsion on sequestration of thermally damaged erythrocytes in rats with experimentally induced hypersplenism, and in normal rats.

The authors attempted at experimental elimination of sequestration function of the spleen in Wistar rats using an i.v. injection of ethyl palmitate emulsion, both in "hypersplenic" animals being long-term applied i.p. methyl cellulose solution, and in control rats. In the rats clearance of 51Cr-labelled and thermally damaged erythrocytes from blood was examined and their sequestration in the spleen and liver followed. The ethyl palmitate injection resulted in both experimental groups in a significant decrease of the erythrocyte counts sequestrated in the spleen, and significant prolongation of the elimination half time for thermally damaged erythrocytes from the blood.

Animals↗
PubMed1978

Incorporation of [14C]-L-leucine in splenic and hepatic tissue of rats "chemically splenectomized" by ethyl palmitate.

In experiments with "chemically splenectomized" rats by an i.v. application of ethyl palmitate (EP) emulsion and using [14C]-L-leucine, the protein synthesis in spleen and liver was followed. Two hours following EP application protein synthesis in the spleen was significantly decreased; after 24 hrs and 4 days the incorporation increased moderately above normal values, and in the subsequent intervals protein synthesis was again lowered. In the liver a significant increase of protein synthesis 24 hrs and 4 days after EP injection was observed. Changes of proteins synthesis in the spleen are evidently related to damage of the tissue and its subsequent regeneration. Changes in the liver are manifestations rather of an increased metabolic load during EP degradation.

Animals↗
PubMed1977

The inhibitory effect of methyl palmitate on heteroagglutinin formation in rats.

In Wistar rats the immunosuppressive effect of methyl palmitate on the formation of heteroagglutinins against human O-group erythrocytes was followed. An i.v. injection of methyl palmitate both delayed the heteroagglutinin formation and decreased its intensity. The inhibitory effect of methyl palmitate was not accompained by the subsequent hyperactive phase as could be observed in the previous experiments using ethyl palmitate.

ABO Blood-Group System↗
PubMed1976

Effects of an i. v. injection of ethyl palmitate emulsion on nucleic acids and fat content of the liver.

In experiments with rats "chemically" splenectomized by an i.v. ethyl palmitate injection the possible side effects on the liver were followed. In the liver proteins, DNA, RNA, esterified fatty acids, and in blood serum transaminases were assessed. In all examinations the variances were only temporary and could be characterized as manifestations of a metabolic load rather than actual liver damage.

Animals↗
PubMed1976

Thermostability of the erythrocytes of mice exposed to the action of a magnetic field.

The authors investigated the thermostability of the erythrocytes os strain CBA mice exposed to the action of a magnetic field formed by the poles of a rotating permanent magnet of 20 mT (200 GAUSS) induction. The erythrocytes of animals which spent 17 weeks in the magnetic field were significantly less heat resistance than those of the control animals. The effect on animals kept in the magnetic field during embryoyenesis and infancy and then left 3 months outside it was even more pronounced.

Animals↗
PubMed1976

[The effect of splenectomy, produced chemically by means of ethylpalmitate, on the formation of heteroagglutinins in the rat].

The formation of heteroagglutinins against human O-erythrocytes was pursued in Wistar rats splenectomized "chemically" by means of intravenous injections of ethyl palmitite. Contrary to some data in literature the initial inhibition in the formation of antibodies was replaced by a significant titre increase ranging from the 8th to the 14th day following immunization. The tween 20 contained in the emulsion of ethyl palmitic did not influence the formation of heteroagglutinins. The examinations show that a possible use of palmitic ethyl for immunosuppression is questionable.

Animals↗
PubMed1975

Blood volume changes in "hypersplenic" rats.

Blood volume in "hypersplenic" and normal rats was assessed by a simultaneous measurement of erythrocyte and plasma volumes by means of 59Fe-labelled erythrocytes and 131I-labelled human serum albumin, respectively. The "hypersplenic" condition was induced by prolonged intraperitoneal application of methylcellulose. Mean blood volume in normal rats was 6.3 ml/100 g body weight, the venous haematocrit being 48%. Mean blood volume in "hypersplenic" rats was 7.5 ml/100 g body weight, and the venous haematocrit lower by 22% than in normal animals. Compared with normal animals, the erythrocyte volume in "hypersplenic" rats was lower by 15% only. Plasma volume in "hypersplenic" rats exceeded the compensation in response to the reduction in erythrocyte mass. In addition to haemolysis, haemodilution due to plasma expansion seemed to be responsible for the anaemia in "hypersplenic" rats.

Animals↗