Biomedical subjects

V V Gusinskaia

Publications and source records attributed to V V Gusinskaia.

5 recordsLinked to original sources

PubMedDate not supplied

[Oligomeric proteins: modulation of UV-sensitivity and characteristics of photochemical transformations].

The results of our research devoted to the study of photophysical and photochemical transformations of some compound proteins of oxidative, antioxidant, transport and immune systems of an organism under conditions of different microenvironment (presence of chemical modificators, the change of pH, temperature, doses and spectral composition of ultraviolet irradiation) have been analyzed. Methods and the degree of modulation of UV-sensitivity of proteins of the mentioned systems have been discussed. Conditions, on which the used chemical agents show photoprotective and photosensitizing effect on protein molecules, have been found.

Photochemistry↗

PubMedDate not supplied

[Effect of lipid peroxide photooxidation on activity of lymphocyte-bound peroxidase].

The UV-light influence (240-390 nm) in the doses range of 75.5 divided by 2265 J/m2 has been studied on structurally functional state of lymphocytes by the method of determination of the TBA-active products, spectrophotometric method and enzyme-linked immunosorbent assay. It has been shown that UV-irradiation dose increase led to the intensification of lipids peroxide photooxidation processes on lymphocytes, accompanied by the increase of their natural peroxidase activity and ability to sorb exogenic peroxidase. The association of peroxidase with the native and UV-irradiated lymphocytes results in the decrease of its catalytic activity.

Horseradish Peroxidase↗

PubMedDate not supplied

[Level of nitric oxide and tumor necrosis factor-alpha production by human blood neutrophils under UV-irradiation].

The effect of the UV-irradiation (75.5-2265 J/m2) on the nitrogen oxide (NO) and the tumor necrosis factor-alpha (TNF-alpha) production by neutrophils of human blood was studied by Griess method based on the accumulation of NO2(-) -ions in the incubation matter and on the enzyme-linked immunosorbent assay (ELISA). UV-irradiation in the dose of 75.5 J/m2 reveals the correctional effect relatively to the native level of NO, providing the maintenance of the physiological homeostasis. The irradiation of the neutrophils by the doses of 755 and 2265 J/m2 increases the concentration of NO metabolites, that might lead to different pathologies. The process of NO-synthesis stopped during the incubation of these cells in the presence of the transcriptional inhibitor of the protein sunthesis - cycloheximid. This suggests that the NO generation by photomodified neutrophils is due to the activation of iNOS synthesis de novo by UV-irradiation. UV-irradiation also has a modulating effect on the level of TNF-alpha production by phagocytes. The resulting effect depends on the native cytokine concentration. The positive correlation between NO and TNF-alpha concentrations in native and irradiated cells in the dose of 755 J/m2 was estimated, that shows close interrelationship between these parameters.

Dose-Response Relationship, Radiation↗

PubMedDate not supplied

[The ratio of the contributions from the direct and indirect actions of UV radiation in changing the functional properties of the complement system].

Ionole antioxidant (5 x 10(-6) M) was administered to haemolytic complement system and its components (i.e. suspension of erythrocytes sensitized by rabbit antibodies and blood serum). The general effect of the complement system photoinactivation (4.53 x 10(2) J/m2) was attributed to the direct effect of UV-light on the blood serum proteins (approximately 68%) and indirect effect via radical products (approximately 32%).

Butylated Hydroxytoluene↗

PubMedDate not supplied

[Structural-functional UV-modifications of C1q factor of the human complement system].

The structural transformations of C1q-protein induced by the UV-light at doses 151 and 2265 J/m2 has been determined by means of acid-basic titration, IR- ans UV-spectrophotometry. These transformations are stipulated by unfolding of its molecules without change of the secondary structure types ratio and accompanies by exposition on aromatic amino acid residues as well as glutamine and asparagine acids, lysine, hydroxylysine and cysteine on the globule surface. The correlation between structural photomodifications and processes of functional activation of C1q has been established.

Amino Acids↗