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Biomedical subjects

V V Klimenko

Publications and source records attributed to V V Klimenko.

At least 19 recordsLinked to original sources
PubMed1992

[The structural-functional changes in the cells of Francisella tularensis strain 15 Gaĭskiĭ during cultivation and sublimational drying].

Instrumental methods of investigation were used for the demonstration of changes in the fatty acid composition of F. tularensis, strain 15 Gaĭskiĭ, during cultivation in solid culture medium, storage after lyophilization, as well as changes in the functioning of the system of membrane-dependent enzymes of the respiratory chain and in the permeability of cell wall membranes by water molecules and NADH after lyophilization. A relationship between the survival rate of F. tularensis cells after lyophilization and stimulation of their endogenic respiration with NADH and succinate was revealed. An increase in residual moisture from 6 to 10-12% was found to intensify the process of lipid peroxidation during the storage of lyophilized F. tularensis cells of strain 15 Gaĭskiĭ.

Bacterial Vaccines↗
PubMed1991

[Temperature control of the crossing-over frequency in Drosophila melanogaster. Effect of infra- and super-optimal shock temperatures in early ontogenesis on the recombination frequency].

Effect of temperature shock treatments (0 and 37 degrees C) in early ontogenesis on recombination frequency was studied in two strains of Drosophila X1 and X2. Recombination frequency under treatment with temperature of 0 degrees C and 37 degrees C (shock treatment), as well as at 14 degrees C and 29 degrees C nonshock treatment was found to be dependent on strain genotype, the chromosomal segments under consideration, developmental stage and the age of individuals analysed. Shock treatments usually increase recombination frequency, whereas nonshock treatments lead to unstable and variable recombination frequencies. A concept of ontogenic homeostasis of recombination has been introduced. It is assumed that the effect of temperature treatments on recombination frequency is indirect--i.e. physiologically mediated.

Animals↗
PubMed1979

[Proliferative activity of the surface foveolar gastric epithelium in acetysalicylic acid injury].

The proliferative activity of gastric mucous cells (GMC) was studied in white mice following the administration of acetylsalicylic acid (200 mg/kg). One hour before sacrifice all the animals received intraperitoneal injection of 3H-thymidine. The mitotic index and index of labeling nuclei were calculated by means of radioautographers 3, 10 and 20 days after administering the drug. Following 3-day exposure to acetylsalicylic acid the proliferative activity of GMC remained unchanged as compared to controls. Long-term administration (10 and 20 days) of the drug produced no increase in the number of erosions, but there was a statistically significant rise in the proliferative activity of GMC. This rise was accounted for by increased number of proliferating cells in the foveated and cervical divisions of the glands as well as due to the extension of the zone of distribution of precursor cells in the gastric glands as far as the basal divisions. These changes may be considered as manifestation of the protective-adaptive reaction of the gastric mucosa in response to the damaging effect of acetylsalicylic acid.

Animals↗
PubMed1978

[Effect of heparin on the activity of liver microsome oxidative enzyme systems in healthy rats and rats with experimental glomerulonephritis].

In experiments on mongrel rats it was found that heparin in doses of 2, 5, and 10 mg/kg had an inducing effect on the oxidative systems of hepatic microsomes: hexenal test period was shortened, cytochrome P450 content increased, relative liver weight rose; the activity of histochemically-detectable NAD-enzymes of hepatocytes became greater. Heparin was capable of considerable (2-3-fold) stimulation of antitoxic activity of the liver reduced in experimental glomerulonephritis. The effect of heparin on the antitoxic function of the liver did not correlate with its effect, on the blood coagulation system.

Animals↗
PubMed1975

Number of cells in diapausing silkworm embryos with different ploidies.

The number of cells in diapausing silkworm embryos of different ploidies was determined. Thermal activation was shown to lead to a decrease in the number of cells in parthenogenetic embryos in comparison with normal, zygotic development. The number of cells in the embryo is a direct function of egg volume and a reciprocal function of embryo ploidy and is affected by the maternal genotype.

Animals↗
PubMedDate not supplied

[Evaluation of the efficacy of anesthesiologic protection in induced abortion].

The efficiencies of modern intravenous anesthetics, analgesics, and hypnotics (dormicum, diprivan, ketamine, and fentanyl) in various combinations for artificial abortions was evaluated. Hemodynamic parameters, autonomic status, and clinical signs of awakening were evaluated. The most effective protection was attained with a combination of diprivan, dormicum, and fentanyl.

Abortion, Induced↗
PubMedDate not supplied

[The production of spread synaptonemal complexes of meiocytes from animals and plants by a drying method].

A spreading technique was used to prepare synaptonemal complexes from animals (Bombyx mori and Mus musculus spermatocytes) and plants (Zea mays microsporocytes). Suspension of hypotonically treated cells was spread over plastic-coated slides and air-dried. Fixed and phosphotungsten acid-stained spread preparations were studied by phase-contrast and electron microscopes. The spreading-through-drying method is more efficient and thereby more preferable as compared to the classical hypophase spreading technique.

Animals↗