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Biomedical subjects

V V Matveev

Publications and source records attributed to V V Matveev.

At least 19 recordsLinked to original sources
PubMed2006

[Local myocardial contractility in patients with coronary heart disease and chronic heart failure].

The aim of the study was to evaluate the peculiarities of local left and right ventricular myocardial contractility in patients with coronary heart disease (CHD) and various degrees of chronic heart failure (CHF) using biventricular radioventriculography (RVG). Local left and right ventricular myocardial contractility was evaluated in 127 patients with CHD and CHF using RVG with a standard procedure of segmentary and phase histogram analysis. The patients were divided into two groups according to left ventricular ejection fraction (LVEF). Group I consisted of 89 CHD patients with NYHA II-III CHF and LVEF of > 40%; group II included 38 CHD patients with NYHA III-IV CHF and LVEF of < 40%. The significant decrease of LVEF in group II was caused by the prevalence of hypo- and akinetic segments in the structure of local contractility. In both groups total LVEF was maintained by lateral wall segments. Right ventricular contractility in patients with CHD and CHF was maintained by anteroseptal segments.

Aged↗
PubMed2005

Protoreaction of protoplasm.

My goal is to describe briefly the universal cellular reaction (UCR) to external actions and agents. This general reaction was the main subject of investigation by the scientific school of the outstanding Russian cytologist, Dmitrii Nasonov (1895-1957). The UCR consists of two phases of complex changes in cellular viscosity and turbidity, in the cell's ability to bind vital dyes, in the resting membrane potential, and in cellular resistance to harmful actions. Works from the Nasonov School have shown that these changes are based on structural-functional transformations of many cell proteins that react uniformly to actions of different physical and chemical nature. In general, these complex changes do not depend on cell type, indicating the universal and ancient nature of the UCR as well as its general biological significance. A new interpretation of the mechanism of the universal reaction is proposed in this paper, and a possible role for contractile proteins in the mechanism of the UCR of muscle cells is presented. In addition, the concept of cell hydrophobicity is introduced. Nasonov's School proposed a concept of physiological standardization that allows comparison of data obtained by different investigators and that will also be described here.

Anesthetics, General↗
PubMed2005

"Fathers" and "sons" of theories in cell physiology: the membrane theory.

The last 50 years in the history of life sciences are remarkable for a new important feature that looks as a great threat for their future. A profound specialization dominating in quickly developing fields of science causes a crisis of the scientific method. The essence of the method is a unity of two elements, the experimental data and the theory that explains them. To us, "fathers" of science, classically, were the creators of new ideas and theories. They were the true experts of their own theories. It is only they who have the right to say: "I am the theory". In other words, they were carriers of theories, of the theoretical knowledge. The fathers provided the necessary logical integrity to their theories, since theories in biology have still to be based on strict mathematical proofs. It is not true for sons. As a result of massive specialization, modern experts operate in very confined close spaces. They formulate particular rules far from the level of theory. The main theories of science are known to them only at the textbook level. Nowadays, nobody can say: "I am the theory". With whom, then is it possible to discuss today on a broader theoretical level? How can a classical theory--for example, the membrane one--be changed or even disproved under these conditions? How can the "sons" with their narrow education catch sight of membrane theory defects? As a result, "global" theories have few critics and control. Due to specialization, we have lost the ability to work at the experimental level of biology within the correct or appropriate theoretical context. The scientific method in its classic form is now being rapidly eroded. A good case can be made for "Membrane Theory", to which we will largely refer throughout this article.

Animals↗
PubMed2004

[Cell shrinkage during apoptosis is not obligatory. Apoptosis of U937 cells induced by staurosporine and etoposide].

A study was made of apoptotic cell shrinkage, which is generally believed to be a hallmark of apoptosis. The two conventional models of apoptosis were used for examination of changes in cell water balance--one is apoptosis caused in human lymphoma cell line U937 by staurosporine, and the other by etoposide. Intracellular water was determined by measuring buoyant density of cells in continuous Percoll gradient. Apoptosis was recognized by microscopy and flow cytometry. Apoptosis caused by staurosporine (1 microM, 4 h) was found to be associated with a decrease in cell water content by almost 24%. In contrast, no decrease in cell water content was observed in U937 cells incubated with etoposide (50 microM, 4 h), in spite of the number of features suggesting the presence of apoptosis, such as the appearance of apoptotic bodies, chromatin condensation and fragmentation and disappearance of S-phase cells in DNA histogram. It is concluded that definition of apoptosis as "shrinkage-necrosis" (Kerr, 1971) needs correcting: the distinction of apoptotic cells involves the absence of swelling, rather than cell shrinkage.

Antineoplastic Agents, Phytogenic↗
PubMed2003

[Water and ion balance in rat thymocytes under apoptosis induced with dexamethasone or etoposide. Ion-osmotic model of cell volume decrease].

Cell ion and water balance was studied with respect to analysis of the osmotic model of apoptotic volume decrease (AVD) in rat thymocytes under dexamethasone (1 microM, 4-6 h) or etoposide (50 microM, 5 h) treatment. Intracellular water content was determined by measurement of cell buoyant density in continuous Percoll gradient, while intracellular potassium and sodium contents were determined by flame emission analysis. Apoptosis was verified by an increase in cell buoyant density, fluorescence of cells stained with Acridine orange and Ethidium bromide (flow cytometry), by changes in the cell cycle and the appearance of sub-diploid peak in the DNA histogram (flow cytometry), and by a decrease in cell size examined with light microscope. A separate fraction of dense cells with reduced size was found to appear after dexamethasone or etoposide treatment. This fraction was considered as apoptotic. An increase in buoyant density of apoptotic cells corresponded to a decrease in cell water content. In apoptotic cells vs. cells with normal buoyant density, the intracellular potassium content was lower, but sodium content was higher. The sum of potassium and sodium contents was lower in apoptotic cells. Taken into account the loss of anions, associated with the loss of cations, the bulk decrease in ions content has been sufficient to be accounted for cell volume decrease on the basis of the ion-osmotic model.

Animals↗
PubMed2002

[Painless myocardial ischemia as a risk factor of constantly relapsing paroxysms of cardiac fibrillation].

A case is reported illustrating relationship between marked coronary atherosclerosis with development of painless myocardial ischemia (PMI) and constantly recurrent paroxysms of cardiac fibrillation (CF). A male 62-year-old patient suffering from paroxysmal CF received cordaron in a dose 200 mg twice a day. The treatment was ineffective until a comprehensive examination (thyroid hormones, echocardiography, Holter ECG monitoring, treadmill test, CT of the coronary arteries) found PMI episodes and severe stenosing atherosclerosis and the treatment was changed for sotalex (80 mg twice a day). Sotalex treatment was effective and stopped recurrences of CF.

Coronary Artery Disease↗
PubMed2001

Differential transcription of ion transporters, NHE1, ATP1B1, NKCC1 in human peripheral blood lymphocytes activated to proliferation.

This work, using RT PCR, studied expression of mRNAs encoding ion transporters, the Na/H antiporter (NHE1), the beta subunit of the Na,K-ATPase pump (ATP1B1), the NaK2Cl symporter (NKCC1), and some proteins unrelated to ion transport: the serum and glucocorticoid dependent kinase (hSGK), beta-actin, a glycolytic enzyme (GAPDH), and regulators of proliferation and apoptosis (p53, Bcl-2) during activation of human lymphocytes with phytohemagglutinin for 4-24 h. Within 24 hours the mRNA levels of NHE1, beta-actin, Bcl-2, and p53 increased by more than 100%, the mRNA levels of ATP1B1, GAPDH, and hSGK, by about 50%, while the mRNA levels of NKCC1 decreased transiently. These results indicate a differential transcriptional control of NHE1, ATP1B1, and NKCC1 following a proliferative stimulus of human lymphocytes.

Carrier Proteins↗
PubMed2001

[Study of "group" expression of mRNA of the ion transporters ATP1B1, NHE1 and NKCC1, beta-actin, glycerophosphosphate dehydrogenase, proteins regulating proliferation and apoptosis of p53, Bcl-2, IL-2 and hSGK kinase at the prereplicative stage of human lymphocyte activation].

Previously, we found no segregation in F2 obtained from crosses between two Dileptus anser clones differing (under the same culture conditions) in their serotypes, i.e. in their immobilization antigens (i-antigens); indeed, all the F2 clones had mixed, i.e. hybrid serotype, being immobilized simultaneously with both immune sera developed against either parental clone (Uspenskaya, Yudin, 2000). Presently, experiments were carried out to see if this unusual phenotype would be re-expressed after a temporary switching off. To switch off both expressed i-antigens, serotype transformation was induced in the F2 clones by shifting the culture temperature from 25 to 17 degrees C. Two weeks later, when the clones returned to the initial temperature conditions, each of them was seen to re-express both parental i-antigens. This result is discussed with reference to the role of i-antigens in regulation of their own expression as has been suggested by some authors.

Actins↗
PubMed2001

[Effects of perstarium on the systolic and diastolic functions of the myocardium in patients with chronic cardiac failure].

The aim of the study was to assess prestarium effects on systolic and diastolic cardiac functions in 38 patients with ischemic heart disease and chronic cardiac failure (NYHA functional class II-IV) by biventricular balanced radioventriculography. Both left and right ventricular diastolic dysfunction was found. A course of prestarium treatment caused different alterations in ventricular contractility. 64.2% patients of those who had right ventricular ejection fraction < 40% achieved an increase in right and left ventricular ejection fraction. Improved diastolic function of the myocardium was registered in 71% patients.

Adult↗
PubMed2000

Evidence of a new type of protein-protein interaction: desensitized actomyosin blocks Ca(2+)-sensitivity of the natural one. A possible model for an intracellular signalling system related to actin filaments.

Actin filaments are certainly believed to function as an intracellular signalling system; however, this is not confirmed by direct evidence. We used a two-layer actomyosin gel with a concentration gradient of the troponin-tropomyosin complex (TT-complex, Ca(2+)-sensitive system) between the two layers. To prepare one layer of the system, natural actomyosin (nAM) rich in TT-complex was used. To prepare the second layer, we used desensitized actomyosin (dAM) without the complex. All experimental studies were made in medium with a low ionic strength. Two phenomena were observed: (1) dAM blocks Ca(2+)-sensitivity of nAM when the dAM weight portion in the system (as well as in mixed nAM + dAM suspension) reaches 40% and more; further increase of the dAM portion does not affect the Ca(2+)-sensitivity; (2) it was electrophoretically shown that a rapid diffusion of the TT-complex from nAM gel into the dAM gel took place. The apparent diffusion coefficient for the TT-complex in dAM gel is about (1-4).10(-4) cm2/sec, i.e. three orders higher than the same values for protein diffusion in water.

Actins↗
PubMed1997

[Hydrophobic interactions as the basis of the inotropic effect of verapamil].

The role of hydrophobic interactions (HI) of verapamil (V) in the mechanism of its blocking action on voltage-dependent Ca(2+)-channels was considered. For this purpose a comparative study of V inotropic effects and general anesthetics (GA) modeling V action on rhythmic switches of frog heart muscle was made. Concentration thresholds of the agents of inotropic action (Cth) were determined at two frequencies of stimulation, 4 and 30 switches per minute, resp. According to features of the inotropic action the studied agents were divided into two groups: Cth values of the former group were dependent on stimulation frequency (V, methanol, ethanol, isopropanol, chloral hydrate and acetone); Cth values of agents of the latter group were independent on stimulation frequency (n-butanol, n-pentanol, n-hexanol, and chloroform). In both groups Cth values were linearly dependent on agent hydrophobicities (in logarithmic scale). It indicates that HI play the main role in interaction of the studied agents with biostructures and primarily with protein structures of Ca(2+)-channels, which are targets for V-like ligand action. The results obtained make an experimental support of our earlier proposed "hydrophobic" model of blocking action of V on plasmalemmal L-type Ca(2+)-channels.

Animals↗
PubMed1992

[Changes in the adhesion between enterocytes in short-term exposures to blastomogens, retinol acetate and indomethacin].

The study was concerned with the effect of carcinogens and anticarcinogens on the rate of cell loss in the large bowel epithelium of mice. Intestinal carcinogens such as N-methyl-N-nitrosourea and 1.2-dimethylhydrazine were shown to cause a long-lasting decrease in enterocyte loss. Conversely, retinol acetate and indomethacin treatment enhanced cell loss both under normal conditions and in the presence of the carcinogens. The protective effect against N-methyl-N-nitrosourea was more apparent than against 1.2-dimethylhydrazine. The effect was more pronounced in Balb/c mice compared to AKR/J. A close correlation between the protective and the anticarcinogenic effects of the drugs was established. The data obtained suggest that retinol acetate and indomethacin cause reversion of specific early reaction of the large bowel epithelium to carcinogens and are most effective in application in the promotion phase of carcinogens.

1,2-Dimethylhydrazine↗
PubMed1991

[Changes in the adhesion between enterocytes in short-term exposures to antioxidants and 1,2-dimethylhydrazine].

Ascorbic acid, alpha-tocopherol acetate, butilated hydroxytoluene, butilated hydroxyphenol, and sodium selenite have no influence on the intercellular adhesion in colon epithelium of mice. These antioxidants can drop or prevent from the increase in colonocyte adhesion induced by a single injection of colon carcinogen--1,2-dimethylhydrazine. With a simultaneous instillation of antioxidants and the colon carcinogen, the protective effect drops to zero in the following line: ascorbic acid--alpha-tocopherol acetate--butilated hydroxytoluene--sodium selenite--butilated hydroxyphenol. On instillation of the antioxidants 8-9 days after single injections of 1,2-dimethylhydrazine, the protective effect dropped to zero in the line: ascorbic acid--sodium selenite--alpha-tocopherol acetate--butilated hydroxytoluene. The data obtained give rise to a conclusion that the investigated effects of antioxidants may correlate with their anticarcinogenic actions.

1,2-Dimethylhydrazine↗
PubMed1989

[Changes in the adhesiveness between enterocytes after short-term blastomogenic exposures].

Following short-term local applications of high doses of N-methyl-N-nitrozourea and 7,2-dimethylbenz(a)antracen, the intercellular adhesion in the distal colon epithelium of ice retains increased at least for a month. The magnitude of this shift and its duration ops both with moving away from the area of application, or with reducing the doses of carcinogens to be closely related to the frequency of tumour production in the site of epithelium under chronic local applications of N-methyl-N-nitrozourea. After the application of noncarcinogenic methylurea or the subcutaneous injection of 1,2-dimethylhydrazine to the mice resistant to its carcinogenic action the wavy fluctuations of the magnitude of intercellular adhesion disappear within a week. The following conclusion can be drawn from the results: the long-term increase in enterocyte-enterocyte adhesion may be an earlier sign of the colon epithelium reaction to carcinogenic action.

1,2-Dimethylhydrazine↗
PubMed1987

[Enhanced stability of isolated muscles and actomyosin due to the action of anesthetics in subnarcotic concentrations].

Subnarcotic concentrations of anesthetics and model anesthetics prolong the survival time of isolated living frog skeletal muscles, the time of contractile ability of the same glycerinated muscles, and the time of frog skeleton muscle actomyosin denaturation. The mode of anesthetic action involves hydrophobic interactions of anesthetics with the investigated biosystems. According to the quantitative analysis, physico-chemical parameters of the anesthetic-binding sites are identical in muscle and in muscle models. This result is interpreted as an evidence that anesthetic-evoked rising of contractile protein stability may be involved in the mechanism of stabilization of isolated muscles.

Actomyosin↗
PubMed1987

[Myosin and actin levels in poorly differentiated skeletal muscle tumors].

Mouse low-differentiated rhabdomyosarcoma (LD RMS) A7 contains 4.5 mg/cm3 of skeletal muscle type myosin and 9.7 mg/cm3 of actin. When recalculated to a volume of cytoplasm of A7 cells, the myosin concentration increases to 12.5 mg/cm3, while that of actin increases to 27.1 mg/cm3. In skeletal muscles the tissue concentrations of myosin and actin is close to the cytoplasmic concentrations. In mouse femoral muscles the tissue and cytoplasmic concentrations of myosin (approximately 102 mg/cm3) and actin (approximately 67 mg/cm3) are 8 (for myosin) and 2.5 times (for actin) higher than the cytoplasmic concentrations of these proteins in tumour cells. The molar ratio of myosin to actin concentrations is 1:26 in the tumour and 1:8 in the muscles. Possible causes of the absence of skeletal muscle type myofibrils and the manifestations of coordination and discoordination of gene expression in LD RMS cells are discussed.

Actins↗
PubMed1986

[Actomyosin from a low-differentiation skeletal muscle tumor].

Actin and subunits of myosin were identified in actomyosin preparations isolated from a low-differentiated rhabdomyosarcoma. Determination was made of Ca2+-ATPase activity and of the ratio of concentrations of tumor myosin light chains. Aggregates were obtained bearing similarity with synthetic filaments. The tumor myosin has all the light chains characteristic of the myosin of definitive fast skeletal muscles, and does not have light chains corresponding to any other myosin isoforms. Quantitative peculiarities of light chain composition of tumor myosin may be explained by peculiarities of cell composition of the tumor. The data obtained indicate that the mechanism coordinating myosin gene expression is extremely resistant to tumoral discoordinating factors. Peculiarities of coordination of the expression of genes coding tissue-specific polypeptides are discussed.

Actins↗