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V V Matveev

Publications and source records attributed to V V Matveev.

30 records · Page 2Linked to original sources
PubMed1984

[Changes in the cytoskeleton and surface charge as a possible cause of the increased mechanical binding between enterocytes in the small intestine epithelium induced by 1,2-dimethylhydrazine].

Alterations in the mechanical linkage between the enterocytes in BALB/c mouse colonic epithelium were investigated under the influence of agents affinitable to the cell surface and cytoskeleton. It is found that cytochalasin B (10(-6) - 10(-5) g/ml), low pH (pH 3) and low concentration of colchicine (10(-8) g/ml) increased, but EDTA (5 X 10(-3) g/ml) and high concentration of colchicine (10(-4) g/ml) reduced the magnitude of mechanical linkage between the enterocytes. A comparative analysis of the data obtained with the control and with 1,2-dimethylhydrazine (DMH) treated mice indicates that probable reasons of a DMH-induced rise in the intercellular mechanical linkage may involve the decrease in electrostatic repulsive forces between the cell surfaces, and the weakening of centripetal tensions due to a decrease in negative surface charge and disorganization of the microfilament system, respectively. These alterations of the cell surface negative charge and the cytoskeleton are expressed on day 3 after a single injection of DMH and are seen to persist during repeated carcinogen applications.

1,2-Dimethylhydrazine↗
PubMed1984

[Kinetic characteristics of the normal enterocyte population and in carcinogenesis].

A correlation was revealed between the crypt cell proliferation changes and variations in adhesion among mature enterocytes at the extrusion zone: an increase in the proliferative activity is accompanied by a decrease in the cell adhesion, and vice versa. This dependence exists in the normal tissue and possibly serves for the epithelium protection from hypo- and hyperplasia. A distinctive feature of carcinogen treatment is the irreversible disturbance of the dependence.

1,2-Dimethylhydrazine↗
PubMed1984

[Actomyosin preparations obtained from a skeletal muscle tumor of low differentiation using the traditional methods of muscle biochemistry].

Tumor actomysion preparations isolated by three different extracting solutions are very similar in composition. A characteristic feature of the preparations obtained is a very rapid presumably proteolytic degradation of their components. It is shown that the major polypeptides of freshly prepared samples are not products of proteolysis. The major protein components of these preparations are identified.

Actomyosin↗
PubMed1984

[Isolation of actomyosin from a skeletal muscle tumor of low differentiation].

A method of actomyosin (AM) isolation from low-differentiated rhabdomyocarcoma (LD RMS) has been worked out. The preparations obtained are sufficiently pure to be used for determination of the composition and some properties of major contractile proteins. Data on the composition of AM preparations isolated from LD RMS by traditional muscular biochemical methods (Matveyev, 1984) and morphological peculiarities of LD RMS cells, which help to distinguish them from definitive skeletal muscle cells and to relate them to non-muscle and low-differentiated muscle cells (mitotic myoblasts), served the main prerequisite to developing the method. Thus, this method may be regarded as a rational basis for isolation of non-muscle and embryonic contractile proteins, but it can be used, probably, for such purposes without special any adjustment to non-muscle or embryonic cells. The cytoplasmic localization has been shown for the tumor myosin.

Actomyosin↗
PubMed1983

[Changes in the epithelial permeability of the large intestine of mice in the process of carcinogenesis].

1,2-dimethylhydrazine (DMH), a colon carcinogen, being injected weekly to BALB/c mice, inhibits an active sodium transport, increases the transepithelial passive ion permeation and decreases ion selectivity in the descending colon. A single DMH injection leads to the same alterations, manifested for a month, followed by normalization of all the parameters to the control value. Distinctive, wavy changes in electrophysiological parameters were noted after a single injection of "non-colon" carcinogen 7,12-dimethyl-benz(alpha)antracen. It is supposed that the prolonged drop in active sodium transport, transepithelial resistance and ion selectivity are specific reactions of the colonic epithelium to carcinogenic treatment with DMH.

1,2-Dimethylhydrazine↗
PubMed1982

[Changes in induced cell loss in the intestinal epithelium of mice after a single exposure to 1,2-dimethylhydrazine].

A single subcutaneous injection of 1,2-dimethylhydrazine lowers the induced cell loss from the superficial regions of mucosa in the distal colon of mice which may be revealed on the 3rd day after injection to retain longer than a month. The magnitude of this effect in the different regions of intestine is closely related to the frequency of tumor appearance established under numerous carcinogen injections. Non-carcinogenous analogs of 1,2-dimethylhydrazine and different carcinogens that induce tumors in other organs, but not in intestine, do not lead to the lowering in the induced cell loss. The data obtained indicate that the above effect may be related to carcinogenous properties of 1,2-dimethylhydrasine.

Animals↗
PubMed1981

[Assessment of cell losses in the intestinal epithelium].

An attempt was made of quantitative evaluation of the epithelial cell separation in vitro from the descending colon of mice (strains A, BALB/C, CBA, C57Bl and random-bred mice) under unified procedure of the tissue desintegration in norm and under carcinogenesis, depending on the age and strain distinctions. The devised method of forced cell isolation enable us to separate single, differentiated enterocytes, being in the stage of desquamation, from intestinal mucose. The cell separation was shown to decrease with age. The BALC/C mice, with high frequency of 1,2-dimethylhydrasine-induced tumor appearance, were characterized by a more decreased cell separation than C57Bl mice, with low sensitivity to the carcinogen. The cell separation from tumor-free mucosal surface in mice with tumors in colon was also seen decreased, as compared with control animals. The data obtained show that the reported approach of forced cell isolation can be useful for the appraisal of cell loss in the intestine, and suggest that the cell loss is decreasing during cancerogenesis.

Aging↗
PubMed1980

[Effect of methanol, ethanol, propanol, chloral hydrate, and potassium chloride on the stability of rabbit skeletal muscle actomyosin].

The denaturation time (DT) of rabbit natural actomyosin (AM) and of actomyosin thread models has been detected under conditions of storage in the presence of methanol, ethanol, propanol, chloralhydrate or potassium chloride. DT--is the period of time during which, with the addition of ATP, actomyosin preparations maintain their ability to superprecipitation, and thread models--to contraction. All the organic substances examined taken in definite concentrations, were shown to increase the actomyosin DT as compared with the control probes. The greatest increase of DT was observed with the concentrations of methanol, ethanol and propanol being 8, 4 and 2% (v/v), resp. A dependence of the actomyosin DT on KCl concentration was noted. The thread model DT increased in the presence of ethanol--4% (v/v), and chloralhydrate--0.1 and 0.2% (v/v).

1-Propanol↗
PubMedDate not supplied

[Effect of verapamil and anesthetics on the level of sodium and potassium ions in frog muscles. Role of hydrophobic interactions].

Verapamil, general and local anesthetics influences on ionic contents of frog sartorius and cardiac muscles in respect with these drugs hydrophobicity were studied to clarify the role of verapamil hydrophobic interactions in its physiological activity. It was found that concentration thresholds of the agents toxicities were linearly linked with their hydrophobicity (in logarithmic scale). This relationship provides support for our conclusion that verapamil, general and some local anesthetics are the members of a single drug family. It is a reason to believe that a hydrophobic mechanism of general anesthetic action on cellular structure is the same as a mechanism of verapamil activity in muscles. It is possible that verapamil is accepted as medical drug exactly due to optimum combination of high verapamil hydrophobicity with its structural complementarity to receptor site responsible for use-dependent channel blocking.

Anesthetics↗