Biomedical subjects

V V Novitskii

Publications and source records attributed to V V Novitskii.

At least 19 recordsLinked to original sources

PubMed2006

Dysregulation of apoptotic death in the pathogenesis of virus-induced cytogenetic instability of blood lymphocytes.

The cytogenetic status and activity of regulatory systems for stability of the cell genome were evaluated in patients with chronic viral persistence. Hepatitis B and C viruses damage the chromosome apparatus of peripheral blood lymphocytes. Cytogenetic instability of immunocompetent cells during chronic viral infection was associated with inhibition of DNA excision repair system and dysregulation of apoptosis in target cells.

Adult↗

PubMed2005

Molecular mechanisms of changes in TNF-alpha production by blood mononuclears in chronic viral hepatitis C.

Chronic viral hepatitis C is associated with decreased production of TNF-alpha by the peripheral blood mononuclear leukocytes irrespective of virus genotype and degree of the morphological activity of the process in the liver. This process positively correlates with the increase in the content of TNF-alpha soluble receptor (molecular weight 55 kDa), which can play a role in the mechanisms of immunopathogenesis of long persistence of hepatitis C virus in the body.

Adolescent↗

PubMed2005

Chronic tick-borne encephalitis virus antigenemia: possible pathogenesis pathways.

Study of the proliferative potential and immunophenotype of lymphocytes and cytokine-producing capacity of mononuclear cells in patients with chronic tick-borne encephalitis virus antigenemia showed changes in T cell lymphoproliferative response, relative content of T cells and T helper inductors, immunoregulatory index, and imbalance in the production of immunoregulatory Th1 and Th2 cytokines.

Adolescent↗

PubMed2005

Apolipoprotein A-I possessing activity of defensin proteins modifies structure of plasma membranes.

We studied the in vitro effect of apolipoprotein A-I possessing activity of defensin proteins on the structure of plasma membranes in donor erythrocytes and lymphocytes. Incubation of erythrocyte membranes with apolipoprotein A-I was accompanied by significant changes in biophysical characteristics of a fluorescent probe pyrene in the hydrophobic membrane region and a decrease in Na+/K(+)-ATPase activity. Microviscosity of annular lipids in the plasma membrane of lymphocytes increased during incubation with apolipoprotein A-I. Our results suggest that membranotropic activity of apolipoprotein A-I is associated with the presence of amphipathic alpha-helix regions.

Apolipoprotein A-I↗

PubMed2005

LPO and apoptosis during pulmonary tuberculosis.

LPO and apoptosis in blood mononuclear cells were studied in patients with pulmonary tuberculosis before and during treatment with standard chemotherapeutics. Pulmonary tuberculosis was accompanied by LPO activation and intensification of apoptosis in lymphocytes and monocytes. These changes were observed before and after the course of intensive care. The intensity of lipid peroxidation retuned to normal, while activity of apoptosis remained high after therapy.

Adult↗

PubMed2004

Volume-dependent regulation of Ca2+-activated potassium channels in erythrocytes from healthy donors and patients with type II diabetes mellitus aggravated by arterial hypertension.

Increase in intracellular Ca2+ concentration caused by calcium ionophore A23187 or ascorbate+phenazine methosulphate electron donor system added to erythrocyte suspension induced similar shifts in erythrocyte membrane potential. These processes are most likely mediated by Ca2+-activated potassium channels. Changes in the osmolarity of the incubation medium produced opposite effects on membrane hyperpolarization induced by A23187 or ascorbate+phenazine methosulphate in erythrocyte isolated from healthy donors, which attests to the existence of different mechanisms of regulation of Ca2+-activated potassium channels. There was no difference in the volume-dependent changes of potassium permeability in cells from patients with type II diabetes mellitus combined with arterial hypertension induced by application A23187 or electron-donor system.

Calcimycin↗

PubMed2004

Typical changes of erythrocytes in chronic inflammation.

Structural and metabolic status and function of erythrocytes were studied in patients with chronic bronchitis, chronic inflammatory diseases of the upper airways, and chronic colitis. Complex study of the peripheral component of the erythron showed that disorders in erythrocyte morphology and function (decreased erythrocyte dry weight, low concentration of sulfhydryl groups and lipoproteins, decreased content of high molecular weight polypeptides in parallel with increased content of low molecular weight proteins, increased number of transformed cells, and enhanced reversible aggregation) were nonspecific and did not depend on the location of the inflammatory process.

Adult↗

PubMed2004

Cytogenetic disorders in peripheral blood lymphocytes of patients with febrile form of tick-borne encephalitis.

An appreciable increase in the number of aberrant lymphocytes was detected in the peripheral blood of patients with febrile form of tick-borne encephalitis (TBE). This increase peaked during week 2 of the infectious process and was paralleled by a decrease in the count of natural killer cells. By the end of the acute period of neuroinfection the number of cells with structural chromosome aberrations decreased, but still surpassed the control (donor blood). Genetically unstable cells were eliminated predominantly at the expense of lymphocytes with chromatid disorders, while the count of CD16-expressing lymphocytes increased. The intensity of reparative DNA synthesis was suppressed during the entire acute period of tick-borne encephalitis.

Adult↗

PubMed2004

Pathomorphosis of erythrocytes in patients with acquired valvular heart diseases and under conditions of their correction.

Scanning electron microscopic study of the morphology and surface architectonics of erythrocytes in patients with acquired aortic and mitral valve diseases showed signs of morphological restructuring of the erythrocyte population. Reversibly transformed transitional red blood cells and irreversibly changed prehemolytical and degenerative erythrocytes were much more incident in these patients than in donors. The number of functionally intact biconcave discocytes notably decreased in comparison with donors. Morphological heterogeneity of the erythrocyte pool increased during the immediate period after replacement of heart valves with mechanical disc prostheses. Disorganization of the surface relief of red blood cells persisted 12-24 months after surgery.

Adult↗

PubMed2004

Electron microscopic characteristics of peripheral blood lymphocytes in children with infectious mononucleosis.

Infectious mononucleosis is associated with pronounced changes in surface architectonics of peripheral blood lymphocytes persisting during convalescence and remote period after the disease. The degree of these changes depends on the disease agent and age-specific characteristics of the body. The most pronounced and sustained disorders in the morphostructural organization of lymphocytes are caused by Epstein-Barr virus (in comparison with agents of other etiology); these disorders are more pronounced in children aged 7-14 years than in those aged 3-6 years.

Adolescent↗

PubMed2003

Phagocytic functions of the blood in patients with acute tick-borne encephalitis.

We studied metabolic and functional activity of peripheral blood phagocytes from patients with febrile tick-borne encephalitis. Phagocytic activity of neutrophils determining nonspecific resistance of the organism progressively increased, while metabolic activity decreased. Secretion of nitric oxide by peripheral blood mononuclear phagocytes was suppressed, while phagocytic functions of these cells remained unchanged.

Acute Disease↗

PubMed2003

Typical changes of reversible erythrocyte aggregation in various pathological processes.

Examinations of patients with malignant tumors of different location (lung cancer, stomach, colorectal cancer, and head and neck tumors), patients with paranoid schizophrenia and neurotic disorders revealed enhanced reversible erythrocyte aggregation. It was found that enhancement of erythrocyte aggregation is associated with structural modification of their membrane and appearance of transformed cells (echinocytes, stomatocytes, etc.). The phenomenon of enhanced reversible erythrocyte aggregation in somatic and mental diseases can be regarded as a typical pathological process.

Adult↗

PubMed2003

Fluorescent probing of lymphocyte and erythrocyte plasma membranes in persistence of tick-borne encephalitis virus: monitoring of structural changes.

The structure of lymphocyte and erythrocyte plasma membrane in patients with long persistence of tick-borne encephalitis virus was studied using fluorescent probes pyrene and 1-anilinonaphthalene-8-sulfonate. The authors analyze the clear-cut disorders in the membranes of both lymphocytes and erythrocytes in viral tick-borne-encephalitis from the viewpoint of generalized involvement of cell membranes in infectious process.

Adult↗

PubMed2003

Structure, metabolism, and functions of peripheral blood lymphocytes during long-term persistence of Epstein-Barr virus.

Study of surface architectonics, blast transformation potential, and cytochemical activity of peripheral blood lymphocytes from children infected with Epstein-Barr virus revealed imbalance between structural, metabolic, and functional state of lymphocytes. This imbalance persists in delayed period after infection and determines long-term viral persistence in the macroorganism.

Adolescent↗

PubMed2003

Structure and properties of lipid bilayer of erythrocyte membranes in patients with malignant tumors.

The study of erythrocyte membranes in untreated patients with malignant tumors of different location (lung cancer, tumors of the head and neck, stomach and colorectal cancer) revealed changes in the fatty-acid spectrum of phospholipid fraction paralleled by an increase in the viscosity of the lipid bilayer, including the area of protein-lipid contacts. The degree of changes depended on tumor location.

Adult↗

PubMed2002

Changes in the lipid phase of erythrocyte membranes in patients with paranoid schizophrenia.

Considerable changes in the lipid phase of erythrocyte membranes were found in patients with paranoid schizophrenia. During exacerbation and remission of the disease the content of phosphatidylethanolamine decreases, while the amount of lysophosphatidylcholine decreases. These changes are accompanied by an increase in erythrocyte membrane microviscosity. Structural modification of the lipid phase in erythrocyte membranes in patients with schizophrenia is associated with intensification of lipid peroxidation.

Adult↗

PubMed2002

Chromosome aberrations, apoptosis, and DNA repair in peripheral blood lymphocytes in children with infectious mononucleosis.

Infectious mononucleosis is associated with suppression of lymphocyte apoptosis and accumulation of cells with chromosome aberrations in the peripheral blood. The type and severity of these aberrations do not depend on the etiological variant of infection and patient age. At the same time in 3-6-year-old children with infectious mononucleosis caused by Epstein-Barr virus we observed activation of DNA repair system in lymphoid cells.

Adolescent↗

PubMed2002

Subpopulation composition of peripheral blood lymphocytes in children with infectious mononucleosis.

In patients with viral and bacterial infectious mononucleosis during the acute period of disease and clinical convalescence blood content of CD72(+)and CD16(+)lymphocytes increased compared to normal. The count of CD8(+)cells increased in viral mononucleosis during convalescence and these changes persist in delayed periods after convalescence. In bacterial mononucleosis the content of CD72(+)lymphocytes return normal 18 months after convalescence.

Adolescent↗