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Biomedical subjects

W G Luttge

Publications and source records attributed to W G Luttge.

At least 55 records · Page 3Linked to original sources
PubMed1977

Further studies on the restoration of estrogen-induced sexual receptivity in ovariectomized mice treated with dihydrotestosterone: effects of progesterone, dihydroprogesterone and LH-RH.

Sexual receptivity in ovariectomized CD-1 mice induced by chronic daily injections of estradiol benzoate (E2B) was inhibited in a dose related fashion by daily injections of dihydrotestosterone (DHT) given concurrently with the E2B. Administration of the progestins, progesterone and dihydroprogesterone (DHP), and of the hypothalamic decapeptide, LH-RH, 6 hr prior to testing restored receptivity to varying degrees in these E2B + DHT treated mice. Of these treatments progesterone was clearly the most effective, followed by LH-RH and finally DHP in restoring estrogen-induced receptivity. Results were discussed in terms of a proposed essential role for LH-RH in the induction of sexual receptivity in mice.

20-alpha-Dihydroprogesterone↗
PubMed1976

Regional and subcellular [3h]estradiol localization in selected brain regions and pituitary of female mice: effects of unlabeled estradiol and various anti-hormones.

Groups of ovariectomized mice were pretreated for 30 min with either vehicle, unlabeled estradiol benzoate or one of the following anti-hormones: cyproterone acetate, CI-628 or CN-69, 725-27. One hour after an I.V. injection of [3H]estradiol the levels of toluence extractable radioactivity retained in the crude nuclear and cytosol fractions of selected brain regions, pituitary and plasma were determined by liquid scintillation spectrometry. The major findings were: (1) In vehicle treated animals [3H]estradiol uptake in the pituitary greatly exceeded that in all brain regions. Within the brain uptake was greatest in the preoptic anterior hypothalamus (POA-AH) and medial basal hypothalamus (MBH) samples. Nuclear uptake exceeded cytosol uptake only in the pituitary and POA-AH and MBH samples. (2) Estradiol benzoate pretreatment greatly reduced nuclear, and to a lesser extent cytosol [3H]estradiol uptake in pituitary, POA-AH and MBH samples. Estradiol benzoate pretreatment also reduced nuclear uptake in the dorsal middle hypothalamus (DMH). (3) The anti-androgen cyproterone acetate, previously shown to have anti-estrogenic effects in female sexual behavior tests, was found to have no effect on nuclear or cytosol [3H]estradiol uptake in brain and pituitary. (4) The anti-estrogen CI-628 was found to reduce nuclear [3H]estradiol uptake in the POA-AH and pituitary samples. It also reduced cytosol [3H]estradiol uptake in the pituitary sample. The anti-estrogen CN-69, 725-27 produced a much greater inhibition of nuclear [3H]estradiol uptake than CI-628 in the POA-AH, MBH, DMH, dorsal posterior hypothalamus and pituitary samples. This anti-estrogen also reduced cytosol [3H]estradiol uptake in the POA-AH, MBH and pituitary samples. (5) A preliminary chromatographic analysis of vehicle control samples indicated that 70-98% of the nuclear radioactivity in target regions such as POA-AH, MBH and pituitary was iso-polar with authentic estradiol, while less than 50% of the radioactivity in plasma and cortex behaved as estradiol.

Animals↗
PubMed1976

Intracerebral implantation of the anti-estrogen CN-69, 725-27: Effects on female sexual behavior in rats.

Ovariectomized adult female rats were stereotaxically implanted with double walled cannulas (22 ga outer and 27 ga inner) and tested for sexual receptivity (lordosis behavior) following SC estradiol benzoate and progesterone priming. Implantation of the anti-estrogen CN-69,725-27 (c. 65 mug) immediately after the first of 3 daily estrogen injections produced a dramatic inhibition of sexual receptivity when these implants were placed in the preoptic and anterior hypothalamic areas. CN-69,725-27 implants had no effect on receptivity when implanted in the middle hypothalamus, mesencephalon or frontal cortex. All inhibitory effects of the anti-estrogen were reversible within one day after removal of the CN-69,725-27 cannula from the brain.

Animals↗
PubMed1975

Alterations of central nervous system protein synthesis in response to spinal cord hemisection.

This investigation explores the central nervous system protein-synthetic responses to hemisection of the spinal cord. Adult male rats hemisected at mid T2 were given intravenous injections of 14C-leucine at 0.7, 30, or 70 days postoperative. Four hours later the T1, T2, T3, and C6 segments of the spinal cord and right and left samples of somatomotor and occipital cortex were removed, weighed, and frozen. These samples were later homogenized and subjected to trichloroacetic acid (TCA) separations. Protein-synthetic activity was expressed as the ratio of the specific activity of the TCA precipitable proteins to the specific activity of the TCA free amino acid pool. In the spinal cord samples protein-synthetic activity was greater than in controls at 7 and 30 days postoperative in the T2 segment and at 30 days postoperative in the C6 and T3 segments. In the cortical samples protein-synthetic activity was greater than in controls in the right and left somatomotor cortex at 30 days postoperative, while it was less than in controls in the left somatomotor cortex at 70 days postoperative. Thus, T2 hemisection leads to initial increases and subsequent decreases in protein-synthetic activity in both spinal cord and somatomotor cortex. These results are discussed in terms of possible regenerative neural reactions occurring in the central nervous system following spinal cord injury.

Animals↗
PubMed1975

Effects of anti-estrogens on testosterone stimulated male sexual behavior and peripheral target tissues in the castrate male rat.

In the first experiment castrated male rats were injected daily with either vehicle or 800 mug testosterone together with either 6 hr pretreatment or concurrent treatment with the anti-estrogens CI-628 (4 mg) or MER-25 (20 mg). The only treatment found to significantly reduce male copulatory behavior was concurrent treatment with CI-628. Anti-estrogen treatment was also found to slightly reduce body weights, increase seminal vesicle weights in response to testosterone and to have no significant effects on androgen stimulated increases in penis weight and length. In the second experiment castrated male rats were injected daily with either vehicle or 500 mug testosterone together with 2.5 mg injections of CI-628 given 6 hr before and concurrent with the androgen injections. The anti-estrogen treatment was found to markedly inhibit the desplay of male sexual behavior, to reduce body weights, and to stimulate seminal vesicle weights. Penile weights and lengths were again not effected by the anti-estrogen therapy. These results were interpreted as supporting the theory that testosterone stimulated male sexual behavior in the rat following its aromatization to estradiol in the brain.

Animals↗
PubMed1975

Stimulation of male and female sexual behavior in gonadectomized rats with estrogen and androgen therapy and its inhibition with concurrent anti-hormone therapy.

In the first experiment gonadectomized male rats were injected daily with various combinations of vehicle (V), estradiol benzoate (E), dihydrotestosterone (D) and/or the anti-androgen cyproterone acetate (CA). The eight treatment combinations consisted of V, E, D, CA, E + D, E + CA, D + CA and E + D + CA. Only those males treated with both E and D were found to display ejaculations. Concurrent treatment with CA completely blocked ejaculatory behavior and it significantly reduced both mount and intromission frequencies. Examination of peripheral androgen target tissues indicated that following stimulation with D, CA effectively reduced seminal vesicle and penile weights, and penile lengths, but it did not reduce penile spines. In the second experiment gonadectomized female rats were treated with two daily injections of E, E + CA or E + the anti-estrogen CI-628. A second set of gonadectomized females received three daily injections of testosterone (T), T + CA or T + CI-628. On the day after completion of these injections all females received progesterone and were tested for sexual receptivity three hours later. Both E and T treated females were found to display the lordotic posture in response to mounting stimulation while both CI-628 and CA were found to block this behavior. In E treated females, CI-628 and CA were also found to reduce uterine weight. Thus, CA was shown to have anti-estrogenic as well as anti-androgenic properties. These results were discussed in terms of the aromatization and 5alpha reduction theories of testosterone action in sexual behavior.

Androgens↗
PubMed1975

Intracerebral prostaglandin E2: effects upon sexual behavior, open field activity and body temperature in ovariectomized female rats.

A single 27 gauge implant of PGE2 into the periventricular region of the hypothalamus resulted in a significant increase in sexual receptivity in estrogen primed, ovariectomized female rats. Open field activity levels were only slightly decreased while rectal body temperature increased significantly over control values. It is postulated that the effects upon sexual receptivity might be mediated by PGE2 stimulated LRF release.

Animals↗
PubMed1975

Maintenance of male sexual behavior by combined treatment with oestrogen and dihydrotestosterone in CD-1 mice.

After castration, adult male sexually experienced CD-1 strain mice were treated with dihydrotestosterone (200 mug/day, DHT), oestradiol benzoate (1 mug/day, OB) or DHT (200 mug/day) plus OB (1 mug/day). Oestradiol benzoate and the combined treatment DHT + OB maintained male sexual behaviour at levels comparable to a group of intact control mice, while DHT m maintained behaviour at a lower level. Having all hormone dosages resulted in a decrease in the number of OB-treated animals mating and a complete loss of mating in the DHT-treated animals. The decrease in dosage did not result in any change in the behavior of the mice receiving both hormones. Adrenalectomy was found to have no effect on the mating behaviour of the OB-treated and untreated animals, but it did reduce the number of DHT+ OB-treated animals mating. Thus, both OB alone and combined DHT+OB treatment can maintain male mating behaviour in castrated CD-1 strain mice and these effects do not appear to be due to the effects of oestradiol on the adrenal.

Adrenal Glands↗