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W Gut

Publications and source records attributed to W Gut.

At least 37 records · Page 2Linked to original sources
PubMed1991

[Preparation of Staphylococcus aureus antigens for evaluation of their immunological reactivity with the human sera by the western blot method].

Extracellular antigens as well as cell wall extracts of 4 S. aureus strains isolated from different kinds of infection were analysed by Western-Blott technique. Materials obtained in two systems of bacteria cultivation (with and without aeration) were compared. Four systems of PAGE (native conditions, with 8.0 M urea, with SDS and SDS after previous reduction of the material with 2-mercaptoethanol) were compared in order to get the best differentiation of proteins and antigens. Immunological reactivity of the antigens mixture with two human sera: highly positive (with three S. aureus antigens in ELISA) from patient with staphylococcal sepsis and negative (from blood donor) were analysed. The best results were obtained after reduction of the cell wall extracted material in SDS-PAGE. The different protein patterns depending on the strain and the method of bacteria cultivation were observed. The standardisation of Western-Blott technique was performed, including titration of the sera to get the best differentiation of the antigens. The difference in immunological reactivity of the positive and negative sera with staphylococcal antigens mixture showed rather quantitative than qualitative character.

Air↗
PubMed1991

[Humoral response to Staphylococcus aureus antigens evaluated by the western blotting method].

Cellular antigens extracted from the cells of four Staphylococcus aureus strains from different kinds of infections (sepsis, osteomyelitis, furunculosis) were analysed by the western blotting technique. Antibiotic sensitivity pattern of the strains was compared. One isolate was found to be MRSA strain. Sera samples from patients of whom strains were isolated and four sera from blood donors (as a control) were used in the investigation. IgG levels for purified staphylococcal antigens (lipase, alpha-toxin and teichoic acid) were estimated. Interaction between extracted bacterial antigens and serum antibodies of IgG class were analysed in homologous and heterologous systems. The most strong immunological reaction of the investigated sera with staphylococcal antigens was observed in the case of homologous system. Serum from sepsis patient was found to be the most reactive serum with all staphylococcal antigens mixtures.

Adolescent↗
PubMed1991

[Antibodies to measles virus in blood sera of individuals who were not verified as having been infected with the virus (years 1984-1989)].

A total of 1302 sera were collected from the cases with eliminated current measles virus infection. The HI antibody titre to measles virus was determined in the sera and the results were analyzed depending of the patient age and the year of collection. Statistic analysis exhibited correlation between incidence of measles infections and changes of the antibody level in population (r = 0.93, Po less than 0.001). Problems of significant decreasing of immunity in high vaccinated populations and revaccination was discussed.

Adolescent↗
PubMed1991

[Interpretation of the results of identification of measles virus antibodies in the serum and cerebrospinal fluid from patients with suspected subacute sclerosing panencephalitis (SSPE)].

Clinical diagnosis of subacute sclerosing panencephalitis+ (SSPE) requires laboratory confirmation relying on an evaluation of immune response in serum and in cerebrospinal fluid (CSF) to measle virus. In our study a comparison of antibody level for this virus by ELISA and haemagglutination inhibition test was made in materials derived from 1396 patients with SSPE, sclerosis multiplex, acute measles++ neuroinfections and other diseases of central nervous system (CNS). Statistical analysis permitted to settle criteria for differentiation of SSPE from remaining diseases of CNS and usefulness for diagnosis of particular methods as well as analysis of results obtained with single sample of serum or CNS. An advantage of ELISA for diagnostic purposes and CSF samples were confirmed.

Antibodies, Viral↗
PubMed1990

Poliovirus type 1 enters the human host through intestinal M cells.

It was demonstrated by electron microscopy that after in vitro incubation of human Peyer's patches with poliovirus type 1, either Sabin or Mahoney strain, poliovirus particles adhere specifically to and are endocytosed by intestinal epithelial M cells. These findings suggest that M cells in humans are the site of poliovirus penetration of the intestinal epithelial barrier.

Adult↗
PubMed1990

[Usefulness of immunoenzyme diagnostic kits and latex tests for isolation of rotaviruses].

The aim of this study was to compare characteristic parameters of 4 diagnostic kits available in Poland-immunoenzymatic Rotazyme II and Enzygnost kits and latex kits Rotalex and Slidex. Studies were performed on 67 samples of feces of children treated because of diarrhea. The sensitivity, specificity, frequency of positive tests, false positives and false negatives and the accuracy of the tests under evaluation were determined. The results obtained were further verified using a reference test electrophoresis of RNA of rotavirus. The highest sensitivity was found for Enzygnost, Rotazyme II and Rotalex, 97%, 92% and 90%, respectively, and the lowest for Slidex- 79%, while the specificity was higher for latex kits than for immunoenzymatic kits. The accuracy of the results was highest for Rotalex kit (92%), next for Enzygnost kit (88%), Rotazyme (87%), and Slidex (84%). The significant correlation between OD value readings in spectrophotometer in Rotazyme II kit and the results of visual reading in latex test was found. All tested kits were found to be useful for diagnostic purposes. Rotalex kit due to the high accuracy of the results obtained, methodological simplicity, short time of testing and relatively low price could be a based test in hospital laboratories.

Adolescent↗
PubMed1990

[Use of peroxidase-labeled antigen of tick-borne encephalitis virus for analysis of specific immunoglobulin response and the evaluation of its diagnostic usefulness for detecting the etiological factor of human neurologic infections].

Peroxidase labelled virus of tick-borne encephalitis (k.z.m.) was used for humoral immune response analysis in humans. Dot-ELISA methods elaborated in a version of "catching antibodies" (cAb-ELISA) was compared in respect to its diagnostic usefulness to indirect immunoenzymatic method (i-ELISA) and to haemagglutination inhibition test (OZHA). High sensitivity and diagnostic value of both methods were confirmed. As compared to i-ELISA, cAb-ELISA was characterised by higher specificity of IgM antibody determination resulting from a lack of influence of rheumatoid factor and specific IgG on above mentioned determinations of antiviral IgM in cAb-ELISA. Moreover, parallel analysis by cAb-ELISA technique of serum and cerebro-spinal fluid permits to evaluate in a simple manner a synthesis of specific viral antibodies present in central nervous system.

Antibodies, Viral↗
PubMed1990

[Subacute sclerosing panencephalitis (SSPE) in Poland 1987-1989. Stage IV of the epidemiological studies].

In the years 1987-1989 111 patients of SSPE who react positively to laboratory tests were found in Poland. The incidence rate of the disease was in 1987-1.30; in 1980-0.98 and in 1989-0.66 per million inhabitants (in the years 1984-1986 the incidence of SSPE was respectively 1.22; 1.05 and 0.9 per million). The authors observed a shift in the incidence peak of SSPE from the age group 8-9 years in 1977-1983 and 10-12 years in 1984-1986 to the age group 13-14 years in 1987-1989. Among 111 SSPE cases only 6 children (5.4) had been vaccinated against measles while the coverage of measles vaccination in Poland reached over 95%.

Adolescent↗
PubMed1985

Cytomegalovirus in the mononucleosis syndrome in children.

In 7 children aged 18 months to 7 years isolated from a group of 128 children with infectious mononucleosis the cytomegalovirus infection was found. Infection was diagnosed by determination of antibodies against immediate early and late CMV antigen by means of the ELISA test. Besides that, antibodies were determined against the capsid antigen and early antigen of EB virus by the method of indirect immunofluorescence. In four children only cytomegalovirus infection was found and three had a mixed infection with both viruses and the diagnosis in these cases was: infectious mononucleosis (due to EBV) with coexistent or following CMV infection. In the sera of two children with cytomegalovirus mononucleosis changes were observed in the antibodies against EBV which is explained as a result of interactions between CMV and EBV in the organism of the host.

Adolescent↗
PubMed1984

Complement and circulating immune complexes in children with mumps meningitis.

Haemolytic activity of complement (CH50) and occurrence of circulating immune, complexes (CIC) were studied in serum samples from children with mumps meningitis taken at regular intervals. The decrease of CH50 was observed in 46 out of 77 samples (60%) in the Ist examination, between 1-6th day from the appearance of symptoms, in 43/67 samples (64%) in the IInd examination, between 12--16th day of disease, and in 7/22 samples (32%) in the IIIrd examination, between 12--14th month of observation since the appearance of symptoms. Typical changes of complement level during the disease were of such profile that the most pronounced decrease was in the Ist and IInd examination with the normalization in the IIIrd examination. The presence of CIC was found in 21%, 15% and 14% of samples in the Ist, IInd, and IIIrd examination respectively. Serum sample form the child on 5th day of disease, with high concentration of CIC (482 micrograms/ml), was subjected to sepharose 6B gel filtration on the calibrated column. The molecular weight of thus analyzed immune complexes was as follows: 2.5 X 10(6) and 5.0 X 10(5) daltons. CIC contained anti-mumps antibodies, determined by Elisa, in IgG, IgA and IgM classes.

Adolescent↗