[Etiopathogenesis and sequelae of viral neuroinfections in children. III. Serodiagnosis of mumps neuroinfections].
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Adolescent↗
Biomedical subjects
W Gut
Publications and source records attributed to W Gut.
[Etiopathogenesis and sequelae of viral neuroinfections in children. II. Etiology and clinical aspects].
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Child↗
Commercial antirabies vaccine used as antigen in a modified enzyme-linked immunosorbent assay.
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Antigens, Viral↗
Radioimmunoassay of measles virus antibodies in SSPE.
A sensitive radioimmunological assay (RIA) was introduced for detection of measles virus IgG and IgM antibodies. The hyperimmune response to measles virus could be demonstrated more precisely by RIA than by haemagglutination inhibition (HI). The ratio between RIA and HI antibody titres was decidedly higher in sera and cerebrospinal fluids (CSF) of patients with subacute sclerosing panencephalitis (SSPE) than in those of other groups tested.
Adolescent↗
Cytomegalovirus antibodies in renal transplant recipients.
The relationship between CMV infections and the development of various viral antibodies were studied in successively taken sera from 5 renal kidney recipients. In all of the cases tested the infections were connected with the development of antibodies to immediately early antigens and then specific IgM and antibodies to various CMV early antigens. The increase of antibodies measured by ELISA, CF and immunofluorescence for late CMV antigens were observed in samples taken from two patients. Based on serological findings the relation between CMV infections and quantity of blood received before transplantation was established.
Antibodies, Viral↗
Detection and differentiation of cytomegalovirus antibodies by radioimmunoassay.
A sensitive radioimmunological method was adapted for the assay of anticytomegalovirus IgG and IgM antibodies. Binding of immunoglobulins G with cytomegalovirus (CMV) antigens was greatly reduced when an antigen prepared by sonication of the infected cells in glycine buffer was used in place of the antigen obtained by freezing and thawing of the cells. The application of labelled serum against the Fc fragment of IgG made it possible to identify selectively the antibodies bound with the virus antigen by antigenic determinants.
Antibodies, Viral↗
IgM fluorescence antibodies in sera of pregnant women exposed to rubella.
A high per cent of false positive results for rubella virus IgM antibodies determined by immune fluorescence were detected in sera of pregnant women. After absorption with Staphylococcus suspension, Cowan 1 strain, and aggregates of human immunoglobulins, the false positive reactions due to IgM antiimmunoglobulin were eliminated.
Absorption↗
Study of interaction between IgG and IgM antibodies against rubella virus by the immunofluorescence method.
In immunoglobulin fractions or after elimination of IgG by absorption the immunofluorescence test for rubella IgM antibodies is more sensitive than in whole serum. Blocking of IgM activity by IgG antibodies was eliminated when the time of incubation of the serum with virus antigen was prolonged. After prolonged incubation higher titres of rubella antibodies were also obtained in the IgM immunoglobulin fractions. Protein A in Staphylococcus aureus suspension effectively absorbs antibodies of IgG class. The IgM antibody titres in absorbed sera of patients infected with rubella were in some cases 2 to 4 times higher than in unabsorbed sera.
Antibodies, Viral↗
IgM antibodies in rubella infections: absorption with staphylococcal protein A of sera examined by immunofluorescence and haemagglutination inhibition.
A similar frequency of positive results for rubella IgM antibodies in the sera of rubella patients and persons contacting such patients was obtained by immunofluorescence after separation of IgM is sucrose gradient and after absorption of the sera with Staphylococcus aureus and immunoglobulin aggregates. Compared to the immunofluorescence method, the haemagglutination method for rubella IgM antibodies performed after absorption of the sera with S. aureus and reduction of immunoglobulins M with 2-mercaptoethanol was less sensitive for serum samples taken in the acute phase of the disease and 5 weeks after the appearance of rubella symptoms.
Absorption↗
The application of different cell lines and virus strains for detection of immunofluorescence rubella antibodies.
Fluorescence antibodies (FA) were titrated in human sera against antigens synthesized in four different infected cell lines: SIRC, RK-13, Vero and BHK-21. The cells infected in suspension by rubella virus attained the optimal concentration of fluorescence staining antigen earlier, fluorescence was more intensive and the titers of the tested human sera were slightly higher than in monolayer cell cultures. FA titers were high and they were correlated with the antibody titers obtained by hemagglutination-inhibition test. Some practical implications of these findings are discussed.
Antibodies, Viral↗
[Subacute sclerosing panencephalitis (SSPE) in Poland in 1996-1999. Phase VII of epidemiologic studies].
In the seventh phase of epidemiological studies of SSPE data were gathered on patients who developed the disease in 1996-1999. In this time period the diagnosis was confirmed in only 10 cases (4 in 1996, 4 in 1997, 0 in 1998 and 2 in 1999). This is a significant reduction of incidence in relation to the preceding stages, in particular to the years 1993-1995 in which 49 new cases were still reported. This is not ruling out the possibility that at the beginning of the years 2,000 cases would be diagnosed in which the first symptoms developed several or more months earlier.
Adolescent↗
[Clinical aspects of herpes simplex meningoencephalitis].
On the basis of the observed 10 cases the authors discuss the diagnostic, clinical and therapeutic problems of herpes encephalomeningitis. The diagnosis in early phase of the disease but already after appearance of psychic and neurological symptoms and signs is based on clinical criteria and also on the result of examination of the cerebrospinal fluid and rapid serological method ELISA. In view of the already available possibilities of virostatic treatment early diagnosis is of utmost importance, for beginning treatment (preferably with Vidarabine) before the development of extensive necrotic brain lesions.
Adult↗
[Evaluation of changes in the epidemiological situation of SSPE in Poland in the years 1977-1991 (in the course of 15 years after the introduction of mandatory antimeasles vaccination)].
The authors present the results of an epidemiological study of SSPE in Poland conducted since 15 years. Since the years covered by the study (1977-1991) included the first period after the introduction of obligatory vaccinations against measles in our country, the most important aim of this analysis was to find out the possible influence of the effect of these vaccinations on SSPE incidence. A comparison of the data obtained in successive stages of the study shows that a slow gradual decrease of the annual incidence of SSPE has been taking place in our country (from at least 1.6 per million before 1983 to 0.69 in 1991). The effect of the vaccinations is visible also in a significant increase of the mean age of SSPE onset which is a result of ever more widespread vaccination of the youngest children. These observations indicate unequivocally that the aetiological factor in SSPE is a wild strain of measles virus and not that used for vaccinations.
Adolescent↗